Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKFW12EY_RS21815 Genome accession   NZ_AP019777
Coordinates   4846903..4847940 (-) Length   345 a.a.
NCBI ID   WP_054759731.1    Uniprot ID   -
Organism   Methylomonas koyamae strain Fw12E-Y     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4841903..4852940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKFW12EY_RS21790 (MKFW12EY_43420) - 4842032..4842883 (-) 852 WP_054759727.1 hypothetical protein -
  MKFW12EY_RS21795 (MKFW12EY_43430) - 4842912..4843082 (-) 171 WP_157199226.1 hypothetical protein -
  MKFW12EY_RS21800 (MKFW12EY_43440) - 4843140..4843733 (-) 594 WP_221053766.1 recombinase family protein -
  MKFW12EY_RS21805 (MKFW12EY_43450) mltB 4844557..4845735 (-) 1179 WP_245006389.1 lytic murein transglycosylase B -
  MKFW12EY_RS21810 (MKFW12EY_43460) pilU 4845753..4846886 (-) 1134 WP_221053767.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MKFW12EY_RS21815 (MKFW12EY_43470) pilT 4846903..4847940 (-) 1038 WP_054759731.1 type IV pilus twitching motility protein PilT Machinery gene
  MKFW12EY_RS21820 (MKFW12EY_43490) - 4848113..4849135 (-) 1023 WP_054759733.1 SPFH domain-containing protein -
  MKFW12EY_RS21825 (MKFW12EY_43500) - 4849334..4849957 (+) 624 WP_221053768.1 hypothetical protein -
  MKFW12EY_RS21830 (MKFW12EY_43520) - 4849954..4851462 (+) 1509 WP_221053769.1 TIM44-like domain-containing protein -
  MKFW12EY_RS21835 (MKFW12EY_43530) - 4851612..4851965 (-) 354 WP_221053770.1 YciI family protein -
  MKFW12EY_RS21840 (MKFW12EY_43540) - 4851962..4852471 (-) 510 WP_054763274.1 SRPBCC family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38324.18 Da        Isoelectric Point: 7.0362

>NTDB_id=73959 MKFW12EY_RS21815 WP_054759731.1 4846903..4847940(-) (pilT) [Methylomonas koyamae strain Fw12E-Y]
MDIAELLTFSVKNKASDLHLSAGLPPMIRVDGDIRRINIPALNHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQDRGAGAVFRTIPSKVLTLEELDAPKFFQELCTKPRGLILVTGPTGSGKSTTLAAMINHINNNDYAHILTVED
PIEFVHESQKSLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRAMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQNL
KELVDKGLITAKAAMTRAVNKDMFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=73959 MKFW12EY_RS21815 WP_054759731.1 4846903..4847940(-) (pilT) [Methylomonas koyamae strain Fw12E-Y]
ATGGATATCGCCGAACTGCTGACGTTTTCCGTCAAGAATAAAGCCTCCGACTTGCACCTTTCCGCCGGTTTGCCGCCGAT
GATACGGGTGGACGGCGATATTCGCCGGATCAACATCCCGGCGCTGAACCATAAGGAAGTGCATGCGCTGATTTACGACA
TCATGAACGACAAACAGCGCCGCGATTACGAGGAGTTTCTGGAAACCGACTTTTCCTTCGCCTTGCCCGGCGTGGCCCGG
TTCCGGGTCAACGCCTTCAACCAGGACCGCGGCGCCGGCGCGGTATTCCGCACCATTCCGTCGAAAGTCCTGACCTTGGA
AGAGCTGGATGCACCCAAGTTTTTCCAGGAATTGTGTACCAAACCGCGCGGCTTGATCCTGGTGACCGGACCGACCGGTT
CCGGTAAATCGACCACGTTGGCGGCGATGATCAACCATATCAACAATAACGATTATGCCCACATCCTGACCGTCGAAGAC
CCGATCGAGTTCGTCCACGAAAGCCAGAAGTCGCTAATCAACCAGCGCGAGGTGCACCGCGATACCTTGGGTTTCAACGA
AGCGTTGCGCTCCGCGTTGCGGGAAGACCCCGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATTCGTTTGG
CGCTGACCGCGGCCGAAACCGGCCACTTGGTGTTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATAGACCGGATT
ATCGACGTGTTCCCGGCGGCCGAGAAAGACATGATCCGCGCCATGCTATCGGAATCGTTGCAAGCGGTTATATCGCAAAC
GCTGTTGAAGAAAGTGGGCGGCGGCCGGATCGCTGCGCACGAGATCATGGTCGGTACCCCGGCGATCCGCAACCTGATCC
GCGAAGCTAAGGTGGCGCAGATGTATTCGGCGATTCAAACCGGGCGCAAGGACGGCATGCAGACGCTGGACCAAAATTTA
AAAGAGTTGGTCGACAAGGGCCTGATTACCGCCAAAGCGGCGATGACCCGAGCCGTCAATAAAGACATGTTCCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

81.232

98.841

0.803
  pilT Acinetobacter baumannii D1279779

80.233

99.71

0.8
  pilT Acinetobacter baumannii strain A118

80.233

99.71

0.8
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Pseudomonas aeruginosa PAK

80.473

97.971

0.788
  pilT Legionella pneumophila strain Lp02

72.674

99.71

0.725
  pilT Legionella pneumophila strain ERS1305867

72.674

99.71

0.725
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.681

0.678
  pilT Vibrio cholerae strain A1552

69.436

97.681

0.678
  pilT Neisseria gonorrhoeae MS11

67.638

99.42

0.672
  pilT Neisseria meningitidis 8013

67.638

99.42

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.516
  pilU Acinetobacter baylyi ADP1

40.346

100

0.406
  pilU Vibrio cholerae strain A1552

40.708

98.261

0.4
  pilU Pseudomonas stutzeri DSM 10701

39.884

100

0.4

Multiple sequence alignment