Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MKFW12EY_RS21810 Genome accession   NZ_AP019777
Coordinates   4845753..4846886 (-) Length   377 a.a.
NCBI ID   WP_221053767.1    Uniprot ID   -
Organism   Methylomonas koyamae strain Fw12E-Y     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4840753..4851886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKFW12EY_RS21780 (MKFW12EY_43410) - 4840982..4841326 (-) 345 WP_221053765.1 hypothetical protein -
  MKFW12EY_RS21785 - 4841448..4841915 (+) 468 WP_172680229.1 hypothetical protein -
  MKFW12EY_RS21790 (MKFW12EY_43420) - 4842032..4842883 (-) 852 WP_054759727.1 hypothetical protein -
  MKFW12EY_RS21795 (MKFW12EY_43430) - 4842912..4843082 (-) 171 WP_157199226.1 hypothetical protein -
  MKFW12EY_RS21800 (MKFW12EY_43440) - 4843140..4843733 (-) 594 WP_221053766.1 recombinase family protein -
  MKFW12EY_RS21805 (MKFW12EY_43450) mltB 4844557..4845735 (-) 1179 WP_245006389.1 lytic murein transglycosylase B -
  MKFW12EY_RS21810 (MKFW12EY_43460) pilU 4845753..4846886 (-) 1134 WP_221053767.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MKFW12EY_RS21815 (MKFW12EY_43470) pilT 4846903..4847940 (-) 1038 WP_054759731.1 type IV pilus twitching motility protein PilT Machinery gene
  MKFW12EY_RS21820 (MKFW12EY_43490) - 4848113..4849135 (-) 1023 WP_054759733.1 SPFH domain-containing protein -
  MKFW12EY_RS21825 (MKFW12EY_43500) - 4849334..4849957 (+) 624 WP_221053768.1 hypothetical protein -
  MKFW12EY_RS21830 (MKFW12EY_43520) - 4849954..4851462 (+) 1509 WP_221053769.1 TIM44-like domain-containing protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42098.36 Da        Isoelectric Point: 6.7811

>NTDB_id=73958 MKFW12EY_RS21810 WP_221053767.1 4845753..4846886(-) (pilU) [Methylomonas koyamae strain Fw12E-Y]
MDFNALLALMVEKRASDLFITAGRPPSMKVNGKVVEVSKTPLTSDQTMKLVLGIMSQRQRDEFENTKECQFALSAHKLGR
FRVSAFTQRDSAGMVLRRIETTIPSAEELHLPPVLKELIMHKRGLVMFVGATGTGKSTSLAALIRHRNENSSGHIISIED
PIEFIHPHQGCIITQREVGLDTESFEVALKNTLRQAPDVILIGEVRTRETMQHAITFAETGHLCVCTLHANNANQALDRI
LHFFPEDMHSQLFMDLSLNLRGIVAQQLIQRADGKGRYPAIEILLNTPLVADLVRKGEVHKLKELMKNSREHGMQTFDQA
LYDLYTAGKISYEDALHAADSRNEVRLMIKLGAENVNFETAGMSLAENDEDGGSLYK

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=73958 MKFW12EY_RS21810 WP_221053767.1 4845753..4846886(-) (pilU) [Methylomonas koyamae strain Fw12E-Y]
ATGGACTTTAACGCGCTATTGGCATTGATGGTGGAAAAACGGGCTTCGGACTTGTTCATCACCGCCGGCCGGCCGCCGTC
GATGAAAGTCAACGGCAAAGTCGTCGAAGTCTCGAAGACGCCGCTGACCAGCGACCAAACCATGAAACTGGTACTCGGCA
TCATGTCGCAGCGCCAGCGCGACGAATTCGAAAACACCAAGGAATGCCAATTTGCGTTGAGCGCACATAAGCTCGGCCGC
TTCCGGGTCAGCGCCTTCACCCAGCGCGATTCCGCCGGCATGGTGTTGCGCCGGATCGAAACCACGATTCCAAGCGCGGA
AGAATTGCATCTGCCGCCGGTGTTGAAAGAATTGATCATGCACAAGCGCGGCCTGGTGATGTTCGTCGGCGCGACCGGTA
CCGGTAAATCGACCTCGTTGGCGGCCTTGATTCGCCACCGCAACGAAAACAGCAGCGGCCACATCATTTCCATCGAAGAT
CCGATCGAGTTCATCCATCCGCACCAGGGCTGCATCATTACCCAGCGCGAAGTGGGGCTGGATACCGAATCGTTCGAAGT
GGCTTTGAAAAACACCCTGCGCCAGGCCCCGGACGTGATTCTGATCGGCGAGGTTCGCACCCGCGAGACGATGCAACACG
CGATTACCTTCGCCGAGACCGGCCATCTCTGCGTCTGCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATT
TTACATTTCTTCCCCGAAGACATGCACAGCCAGTTGTTCATGGATTTGTCGCTGAATCTGCGCGGGATCGTCGCCCAACA
ATTGATCCAACGCGCCGACGGCAAGGGCCGCTATCCGGCCATCGAAATTCTGTTGAATACGCCGTTGGTCGCCGACCTGG
TGCGCAAAGGCGAAGTGCATAAATTGAAGGAGCTGATGAAAAACTCGCGCGAGCATGGCATGCAGACTTTCGACCAGGCG
CTTTACGATCTGTACACAGCCGGTAAAATTAGTTACGAAGATGCTCTACACGCTGCCGACTCCCGCAACGAAGTCCGCCT
GATGATCAAACTGGGGGCCGAGAACGTCAATTTCGAAACCGCCGGCATGTCGCTGGCCGAGAACGACGAAGACGGAGGCA
GCCTTTATAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.979

100

0.687
  pilU Acinetobacter baylyi ADP1

65.07

94.164

0.613
  pilU Vibrio cholerae strain A1552

57.143

92.838

0.531
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.678

92.308

0.403
  pilT Legionella pneumophila strain Lp02

42.183

89.92

0.379
  pilT Legionella pneumophila strain ERS1305867

42.183

89.92

0.379
  pilT Pseudomonas aeruginosa PAK

40.413

89.92

0.363

Multiple sequence alignment