Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACRAD_RS11035 Genome accession   NZ_AP019740
Coordinates   2356609..2357646 (+) Length   345 a.a.
NCBI ID   WP_005018932.1    Uniprot ID   -
Organism   Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2351609..2362646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACRAD_RS11025 (ACRAD_21640) - 2352128..2355730 (+) 3603 WP_005027475.1 AAA family ATPase -
  ACRAD_RS11030 (ACRAD_21650) - 2355790..2356485 (-) 696 WP_005027477.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACRAD_RS11035 (ACRAD_21660) pilT 2356609..2357646 (+) 1038 WP_005018932.1 type IV pilus twitching motility protein PilT Machinery gene
  ACRAD_RS11040 (ACRAD_21670) pilU 2357675..2358793 (+) 1119 WP_005018931.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACRAD_RS11045 (ACRAD_21680) fur 2358858..2359295 (-) 438 WP_005018930.1 ferric iron uptake transcriptional regulator -
  ACRAD_RS11050 (ACRAD_21690) - 2359407..2359805 (+) 399 WP_005027479.1 outer membrane protein assembly factor BamE -
  ACRAD_RS11055 (ACRAD_21700) - 2359851..2360174 (-) 324 WP_005027482.1 RnfH family protein -
  ACRAD_RS11060 (ACRAD_21710) - 2360174..2361256 (-) 1083 WP_005027483.1 membrane protein -
  ACRAD_RS11065 (ACRAD_21720) - 2361407..2361853 (-) 447 WP_005404364.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38367.06 Da        Isoelectric Point: 6.5776

>NTDB_id=73752 ACRAD_RS11035 WP_005018932.1 2356609..2357646(+) (pilT) [Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEELLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGQIFRDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPSEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KTLVSKGVISPQTARMAAKQPEAFM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=73752 ACRAD_RS11035 WP_005018932.1 2356609..2357646(+) (pilT) [Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413]
ATGGATATTACAGAACTTTTGGCATTCTCGGTTAAAAATGGTGCCTCAGACTTACATCTTTCAGCAGGAATGCCGCCGAT
GATCCGGGTCGATGGAGAAGTCCGCCGTATAAATCTGCCGGCACTTGAGCATAAGGATGTACACCGTCTGGTCTATGACA
TTATGAATGACAAGCAGCGCCGTGACTATGAAGAACTGCTGGAAACCGATTTTTCTTTTGAAGTTCCCGGTGTCGCCCGT
TTCCGTGTGAATGCATTTAATCAGAACCGTGGAGCTGGTGCAGTCTTCCGGACCATTCCATCGAAAGTACTGACTATGGA
AGATCTAGGCATGGGCCAAATCTTCCGTGATATCTGTGACTACCCACGTGGTATTGTTCTGGTTACTGGCCCGACAGGTT
CGGGTAAGTCAACCACGCTGGCAGCAATGCTCGATTATATTAATGAAAACCGTTATGACCATATTTTAACAGTAGAAGAT
CCGATTGAATTCGTACATCAGTCTAAAAAATGCCTGATTAACCAGCGTGAAGTCCACCGTGACACACATGGCTTTAATGA
AGCCCTGCGCTCAGCCTTACGTGAAGATCCGGATATTATCCTGGTAGGTGAGATGCGTGATCTTGAAACTATCCGTCTGG
CACTGACTGCGGCAGAAACTGGTCACCTGGTATTTGGTACCCTGCATACCACATCAGCTGCAAAAACAATTGACCGTGTA
ATTGACGTATTTCCTTCTGAAGAAAAAGATATGGTTCGTGCCATGCTATCTGAGTCACTTCAGGCTGTGATTTCACAAAC
ACTCCTCAAGAAAAATGGTGGTGGTCGTGTAGCCGCCCATGAAATCATGATCGGAATACCGGCCATCCGTAACCTGATAC
GTGAGAATAAAGTTGCTCAAATGTATTCTGCTATTCAGACCGGCGCCAACCATGGTATGACTACCCTTGACCAAAGCCTG
AAAACTTTAGTCTCTAAAGGCGTCATCAGCCCGCAAACTGCCCGTATGGCAGCAAAACAGCCCGAAGCTTTTATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

97.101

100

0.971
  pilT Acinetobacter baumannii D1279779

96.812

100

0.968
  pilT Acinetobacter baumannii strain A118

96.812

100

0.968
  pilT Acinetobacter baylyi ADP1

87.791

99.71

0.875
  pilT Pseudomonas stutzeri DSM 10701

84.302

99.71

0.841
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.386

96.232

0.687
  pilT Vibrio cholerae strain A1552

71.386

96.232

0.687
  pilT Neisseria meningitidis 8013

67.246

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.67
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Pseudomonas stutzeri DSM 10701

39.823

98.261

0.391
  pilU Acinetobacter baylyi ADP1

38.192

99.42

0.38

Multiple sequence alignment