Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACRAD_RS11040 Genome accession   NZ_AP019740
Coordinates   2357675..2358793 (+) Length   372 a.a.
NCBI ID   WP_005018931.1    Uniprot ID   A0A062BU50
Organism   Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2352675..2363793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACRAD_RS11030 (ACRAD_21650) - 2355790..2356485 (-) 696 WP_005027477.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACRAD_RS11035 (ACRAD_21660) pilT 2356609..2357646 (+) 1038 WP_005018932.1 type IV pilus twitching motility protein PilT Machinery gene
  ACRAD_RS11040 (ACRAD_21670) pilU 2357675..2358793 (+) 1119 WP_005018931.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACRAD_RS11045 (ACRAD_21680) fur 2358858..2359295 (-) 438 WP_005018930.1 ferric iron uptake transcriptional regulator -
  ACRAD_RS11050 (ACRAD_21690) - 2359407..2359805 (+) 399 WP_005027479.1 outer membrane protein assembly factor BamE -
  ACRAD_RS11055 (ACRAD_21700) - 2359851..2360174 (-) 324 WP_005027482.1 RnfH family protein -
  ACRAD_RS11060 (ACRAD_21710) - 2360174..2361256 (-) 1083 WP_005027483.1 membrane protein -
  ACRAD_RS11065 (ACRAD_21720) - 2361407..2361853 (-) 447 WP_005404364.1 bacteriohemerythrin -
  ACRAD_RS11070 (ACRAD_21730) - 2362138..2362986 (-) 849 WP_005027485.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41698.94 Da        Isoelectric Point: 6.5784

>NTDB_id=73753 ACRAD_RS11040 WP_005018931.1 2357675..2358793(+) (pilU) [Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413]
MDFNDLLNLMMEQKASDLFITADVEPSMKINGQIVPVSKNKLTGEIVGQLIHAIMTDKQRQEFADTRECNFAITNREKTA
RFRVSAFQQRDLPGMILRRIETQIPTMDDLKLPQVLKELAMTKRGIIIFVGATGTGKSTSLASMIGYRNHNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTASFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IVHFFEADRHSQLYMDLSLNLKAVIAQQLIPTLDGNGRRAAIEVLINTPLLADYIRKGEVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAGQISYKDALKHADAPNDLRLQIKLSEEGASQLMAAGRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=73753 ACRAD_RS11040 WP_005018931.1 2357675..2358793(+) (pilU) [Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413]
ATGGACTTTAATGACTTGCTTAACCTGATGATGGAACAAAAAGCATCGGATCTGTTTATTACTGCCGATGTTGAACCGTC
AATGAAGATTAATGGCCAGATTGTTCCGGTTTCAAAAAACAAACTGACTGGTGAGATTGTCGGACAGCTAATTCATGCAA
TTATGACCGATAAACAGCGGCAGGAATTTGCAGATACACGTGAATGCAACTTTGCTATCACTAACCGGGAAAAAACAGCC
CGTTTTCGGGTCAGCGCCTTTCAGCAGCGTGATTTACCCGGCATGATTTTACGCCGGATTGAAACACAGATTCCAACAAT
GGATGACCTGAAACTGCCACAGGTGTTAAAAGAACTGGCTATGACCAAGCGCGGCATTATTATTTTCGTGGGTGCCACCG
GTACAGGTAAGTCAACCTCGCTTGCTTCAATGATTGGTTATCGGAACCATAATTCCAAAGGTCACATTATTACCATTGAA
GATCCGATTGAATTTGTTCATGAACATGCCGGCTGTATTATTACTCAGCGTGAAGTAGGTATTGATACAGCATCCTTTGA
AATTGCGCTAAAAAATACGCTGCGACAAGCACCTGATGTTATTTTAATCGGGGAAATTCGTTCACGGGAAACAATGGATT
ATGCGATTGCATTTGCAGAGACTGGTCACCTGGTCCTGGCAACTTTACATGCCAATAATGCCAATCAGGCACTAGATCGT
ATTGTCCATTTCTTTGAGGCAGACCGGCATAGCCAGCTGTATATGGATTTATCACTGAACTTAAAAGCGGTAATTGCCCA
GCAGTTGATTCCTACTCTAGATGGTAATGGCCGGCGTGCTGCAATTGAAGTCCTGATCAATACCCCTCTGCTTGCAGATT
ACATCCGTAAAGGCGAAGTACATGAAATCAAGGACCTGATGAAGCGTTCACGTGAATTGGGTATGCAGACGTTTGACCAG
GCACTATATGATTTGTATAAGGCCGGTCAGATTAGTTACAAAGATGCCCTTAAACATGCCGATGCTCCAAATGATTTACG
TCTGCAAATCAAACTGTCCGAAGAAGGGGCCAGTCAGTTAATGGCTGCAGGACGTAATATTACTTTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A062BU50

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.984

100

0.782
  pilU Pseudomonas stutzeri DSM 10701

69.272

99.731

0.691
  pilU Vibrio cholerae strain A1552

58.12

94.355

0.548
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.618

93.011

0.387
  pilT Pseudomonas aeruginosa PAK

40.462

93.011

0.376
  pilT Pseudomonas stutzeri DSM 10701

40.173

93.011

0.374
  pilT Legionella pneumophila strain ERS1305867

40.533

90.86

0.368
  pilT Legionella pneumophila strain Lp02

40.533

90.86

0.368
  pilT Acinetobacter nosocomialis M2

39.942

92.204

0.368
  pilT Acinetobacter baumannii D1279779

39.942

92.204

0.368
  pilT Acinetobacter baumannii strain A118

39.942

92.204

0.368
  pilT Neisseria gonorrhoeae MS11

39.412

91.398

0.36
  pilT Neisseria meningitidis 8013

39.412

91.398

0.36

Multiple sequence alignment