Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCT51_RS20325 Genome accession   NZ_CP107009
Coordinates   4369605..4370639 (-) Length   344 a.a.
NCBI ID   WP_263581599.1    Uniprot ID   -
Organism   Halomonas sp. LR3S48     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4364605..4375639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCT51_RS20310 (OCT51_20310) tyrS 4365945..4367144 (-) 1200 WP_263581596.1 tyrosine--tRNA ligase -
  OCT51_RS20315 (OCT51_20315) - 4367258..4368388 (+) 1131 WP_263581597.1 anhydro-N-acetylmuramic acid kinase -
  OCT51_RS20320 (OCT51_20320) pilU 4368431..4369573 (-) 1143 WP_263581598.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCT51_RS20325 (OCT51_20325) pilT 4369605..4370639 (-) 1035 WP_263581599.1 type IV pilus twitching motility protein PilT Machinery gene
  OCT51_RS20330 (OCT51_20330) - 4370718..4371428 (+) 711 WP_263581600.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCT51_RS20335 (OCT51_20335) proC 4371488..4372312 (+) 825 WP_263581601.1 pyrroline-5-carboxylate reductase -
  OCT51_RS20340 (OCT51_20340) - 4372368..4372958 (+) 591 WP_263581602.1 YggT family protein -
  OCT51_RS20345 (OCT51_20345) metX 4373008..4374165 (+) 1158 WP_263581603.1 homoserine O-succinyltransferase MetX -
  OCT51_RS20350 (OCT51_20350) metW 4374165..4374767 (+) 603 WP_263584027.1 methionine biosynthesis protein MetW -
  OCT51_RS20355 (OCT51_20355) - 4374805..4375266 (+) 462 WP_263581604.1 DUF4426 domain-containing protein -
  OCT51_RS20360 (OCT51_20360) - 4375270..4375599 (+) 330 WP_263581605.1 Rieske (2Fe-2S) protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38158.62 Da        Isoelectric Point: 6.4225

>NTDB_id=737347 OCT51_RS20325 WP_263581599.1 4369605..4370639(-) (pilT) [Halomonas sp. LR3S48]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMEDREVRKLIYDIMGDRQRRDYEEFFETDFSFEVPGVAR
FRVNAFNQARGAGAVFRTIPSEVLTMEDLELGDVFRRLALLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRYDHILTIED
PVEFVHRSKRCLINQREVHRDTHGFAQALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGEEKAMVRSMLSESLQAVISQTLLKRRSGGRVAAHEILVANAAVRNLIREDKVAQIYSAIQTGGNIGMQTLDAAL
AKLVAENVVNRDEAQARAKSALTS

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=737347 OCT51_RS20325 WP_263581599.1 4369605..4370639(-) (pilT) [Halomonas sp. LR3S48]
ATGGATATTACCGAACTGCTGGCGTTCTCGGCAAAGCAGAACGCCTCCGACCTGCATTTATCGGCCGGCTTGCCGCCGAT
GATTCGTGTCGATGGCGATATTCGCCGTCTCAATGTGCCGGCCATGGAGGACCGTGAAGTCCGCAAGCTGATCTACGACA
TCATGGGTGACCGCCAGCGCCGTGACTACGAGGAGTTCTTCGAGACCGACTTCTCCTTCGAAGTCCCCGGCGTTGCGCGT
TTCCGGGTCAACGCCTTCAACCAGGCCCGCGGGGCCGGCGCGGTGTTCCGTACCATCCCCAGCGAGGTGCTCACCATGGA
GGACCTGGAACTGGGCGATGTCTTCCGCCGCCTGGCACTGCTGCCGCGTGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGAGCACGACCCTGGCCGCCATGATCGACTATATCAACGATCATCGATACGACCACATCCTCACCATCGAGGAT
CCGGTGGAGTTCGTGCATCGCAGCAAGCGCTGCCTGATCAACCAGCGCGAGGTGCATCGTGACACCCACGGTTTCGCCCA
GGCGCTGCGCAGCGCCTTGCGCGAGGACCCGGATGTGATCCTGGTCGGCGAGATGCGCGACCTCGAGACCATCCGGCTGG
CGCTCACCGCCGCCGAGACTGGCCATCTGGTATTCGGCACCCTGCATACCACCTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCGAGGAGAAGGCCATGGTGCGCTCGATGCTGTCGGAGTCGCTGCAGGCGGTGATTTCCCAGAC
ACTGCTCAAGCGCCGCAGCGGCGGGCGAGTGGCGGCCCACGAGATCCTTGTCGCCAATGCGGCGGTGCGCAACCTCATCC
GTGAGGACAAGGTGGCGCAGATCTATTCGGCCATCCAGACCGGCGGCAATATCGGCATGCAGACCCTCGATGCCGCCCTG
GCCAAGCTGGTGGCCGAGAACGTGGTCAATCGCGACGAGGCCCAGGCCCGCGCCAAGAGTGCGCTGACCAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.056

98.547

0.779
  pilT Pseudomonas aeruginosa PAK

78.761

98.547

0.776
  pilT Acinetobacter baumannii D1279779

78.171

98.547

0.77
  pilT Acinetobacter baumannii strain A118

78.171

98.547

0.77
  pilT Acinetobacter nosocomialis M2

77.876

98.547

0.767
  pilT Acinetobacter baylyi ADP1

77.353

98.837

0.765
  pilT Legionella pneumophila strain Lp02

71.893

98.256

0.706
  pilT Legionella pneumophila strain ERS1305867

71.893

98.256

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

66.667

99.419

0.663
  pilT Neisseria gonorrhoeae MS11

66.374

99.419

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.572

98.547

0.538
  pilU Pseudomonas stutzeri DSM 10701

41.228

99.419

0.41
  pilU Vibrio cholerae strain A1552

40.936

99.419

0.407
  pilU Acinetobacter baylyi ADP1

39.474

99.419

0.392