Detailed information NTG0737346

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OCT51_RS20320
• Genome accession: NZ_CP107009
• Coordinates: 4368431..4369573 (-)
• Length: 380 a.a.
• NCBI ID: WP_263581598.1
• Uniprot ID: -
• Organism: Halomonas sp. LR3S48
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4363431..4374573

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OCT51_RS20310 (OCT51_20310)	tyrS	4365945..4367144 (-)	1200	WP_263581596.1	tyrosine--tRNA ligase	-
OCT51_RS20315 (OCT51_20315)	-	4367258..4368388 (+)	1131	WP_263581597.1	anhydro-N-acetylmuramic acid kinase	-
OCT51_RS20320 (OCT51_20320)	pilU	4368431..4369573 (-)	1143	WP_263581598.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OCT51_RS20325 (OCT51_20325)	pilT	4369605..4370639 (-)	1035	WP_263581599.1	type IV pilus twitching motility protein PilT	Machinery gene
OCT51_RS20330 (OCT51_20330)	-	4370718..4371428 (+)	711	WP_263581600.1	YggS family pyridoxal phosphate-dependent enzyme	-
OCT51_RS20335 (OCT51_20335)	proC	4371488..4372312 (+)	825	WP_263581601.1	pyrroline-5-carboxylate reductase	-
OCT51_RS20340 (OCT51_20340)	-	4372368..4372958 (+)	591	WP_263581602.1	YggT family protein	-
OCT51_RS20345 (OCT51_20345)	metX	4373008..4374165 (+)	1158	WP_263581603.1	homoserine O-succinyltransferase MetX	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42718.87 Da
• Isoelectric Point: 6.0988

>NTDB_id=737346 OCT51_RS20320 WP_263581598.1 4368431..4369573(-) (pilU) [Halomonas sp. LR3S48]
MKAQEWLEQLLDIMARQEASDLLISVQAPPTIKTAGKLTALGDQRLSVEQVRELVNTAVPEGLKERFLQEREANFALSLS
GRGRFRVSAFQQRNQWAMVIRRIAYEIPHLEELSLPPQLGELANMKRGLVFVVGGTGTGKSTTLASMIQQRNETVGGHII
SVEDPIEYVHPHKKAIINQREVGIDTESFEVALKNTLRQAPDVVLIGEVRTRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPHERHEQIWLDLSLNLHAIVAQQLLPTRDGGRTPVIEILLRTPLIADLIRKGDVGEIKNVMARSRDQGMQTF
DQALFDLYKAGKISEEVAMVHADSANELRMMIKYGDEKSDVLTKAQEGVQRFSLRDDDDF

Nucleotide

• Length: 1143 bp

>NTDB_id=737346 OCT51_RS20320 WP_263581598.1 4368431..4369573(-) (pilU) [Halomonas sp. LR3S48]
ATGAAAGCACAAGAATGGCTGGAGCAATTGCTTGACATCATGGCCCGCCAGGAGGCCTCGGACCTGCTGATCTCGGTGCA
GGCGCCACCCACGATCAAGACTGCCGGCAAGCTCACGGCGCTCGGCGATCAGCGGCTCTCGGTGGAGCAGGTGCGTGAGC
TGGTCAATACCGCCGTACCGGAGGGGCTCAAGGAACGCTTCTTGCAGGAGCGCGAGGCCAATTTCGCGCTGAGCCTTTCC
GGTCGTGGGCGTTTCCGGGTCAGCGCCTTCCAGCAGCGCAACCAGTGGGCCATGGTCATCCGTCGCATTGCCTATGAGAT
ACCTCACCTCGAGGAGCTCTCCCTGCCGCCGCAACTGGGCGAGCTGGCCAACATGAAACGCGGGTTGGTGTTCGTAGTGG
GCGGTACCGGCACCGGCAAGTCGACTACCCTGGCGTCGATGATCCAGCAGCGCAACGAGACCGTGGGCGGGCATATCATC
AGCGTTGAAGATCCCATCGAATACGTGCATCCGCACAAGAAGGCGATCATCAATCAGCGCGAGGTGGGCATCGATACCGA
GTCGTTCGAGGTGGCGTTGAAGAATACCCTGCGCCAGGCGCCGGATGTGGTCCTGATCGGCGAGGTGCGTACCCGCGAGA
CCATGGAGCATGCGCTGACCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACCCTTCACGCCAACAACGCCAACCAGGCG
CTGGACCGCATCATTCACTTCTTCCCCCACGAGCGACATGAACAGATCTGGCTCGACCTGTCGCTCAACCTGCATGCCAT
CGTTGCCCAGCAACTGCTGCCGACCCGCGACGGCGGGCGCACCCCGGTCATCGAGATCCTCTTGCGCACACCTCTGATCG
CCGACTTGATCCGCAAGGGTGACGTAGGCGAGATCAAGAACGTCATGGCCCGCTCGCGCGACCAGGGCATGCAGACCTTC
GATCAGGCGCTGTTCGATCTCTACAAGGCCGGCAAGATCAGCGAGGAGGTAGCCATGGTGCACGCCGACTCGGCCAACGA
GCTGCGCATGATGATCAAGTACGGCGACGAGAAGAGCGACGTGCTGACGAAGGCGCAGGAGGGCGTGCAGCGGTTCTCGC
TACGCGACGACGACGACTTCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (16-274)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.16	98.421	0.592
pilU	Vibrio cholerae strain A1552	58.908	91.579	0.539
pilU	Acinetobacter baylyi ADP1	55.493	93.421	0.518
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	45.37	85.263	0.387
pilT	Neisseria meningitidis 8013	42.121	86.842	0.366
pilT	Neisseria gonorrhoeae MS11	41.818	86.842	0.363