Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCT48_RS01055 Genome accession   NZ_CP107008
Coordinates   221477..222511 (+) Length   344 a.a.
NCBI ID   WP_263590957.1    Uniprot ID   -
Organism   Halomonas sp. M4R1S46     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 216477..227511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCT48_RS01025 (OCT48_01025) - 216898..217347 (-) 450 WP_263590952.1 DUF4426 domain-containing protein -
  OCT48_RS01030 (OCT48_01030) metW 217386..217997 (-) 612 WP_263592552.1 methionine biosynthesis protein MetW -
  OCT48_RS01035 (OCT48_01035) metX 217997..219154 (-) 1158 WP_263590953.1 homoserine O-succinyltransferase MetX -
  OCT48_RS01040 (OCT48_01040) - 219192..219785 (-) 594 WP_263590954.1 YggT family protein -
  OCT48_RS01045 (OCT48_01045) proC 219821..220645 (-) 825 WP_263590955.1 pyrroline-5-carboxylate reductase -
  OCT48_RS01050 (OCT48_01050) - 220704..221423 (-) 720 WP_263590956.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCT48_RS01055 (OCT48_01055) pilT 221477..222511 (+) 1035 WP_263590957.1 type IV pilus twitching motility protein PilT Machinery gene
  OCT48_RS01060 (OCT48_01060) pilU 222538..223680 (+) 1143 WP_263590958.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCT48_RS01065 (OCT48_01065) - 223968..225098 (-) 1131 WP_263590959.1 anhydro-N-acetylmuramic acid kinase -
  OCT48_RS01070 (OCT48_01070) tyrS 225212..226411 (+) 1200 WP_263590960.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38058.61 Da        Isoelectric Point: 6.3162

>NTDB_id=737312 OCT48_RS01055 WP_263590957.1 221477..222511(+) (pilT) [Halomonas sp. M4R1S46]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMEDREVRKLIYDILADRQRRDFEEFWETDFSFEVPGVSR
FRVNVFNQARGAGAVFRTIPSEVWTMEDLGMGDVFRRLSMLPRGLVLVTGPTGSGKSTTLAAMIDYVNENRFEHILTIED
PVEFVHRSKRCLVNQREVHRDTHGFAPALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGDEKAMVRSMLSESLQAVISQALLKRQGGGRVAAHEILIATAAVRNLIREDKVAQIYSAIQTGGNLGMQTLDASL
ARLVADNVVAREEAQAKAKAVLAS

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=737312 OCT48_RS01055 WP_263590957.1 221477..222511(+) (pilT) [Halomonas sp. M4R1S46]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAGCAGAACGCCTCGGACCTGCATCTGTCGGCGGGTCTGCCGCCGAT
GATTCGTGTCGATGGCGATATCCGCCGGCTCAACGTGCCGGCCATGGAGGACCGGGAGGTCCGCAAACTGATCTACGACA
TCCTGGCCGATCGCCAGCGCCGGGACTTCGAGGAGTTCTGGGAGACCGACTTCTCCTTCGAGGTGCCGGGCGTCTCGCGC
TTTCGTGTCAACGTCTTCAACCAGGCCCGCGGCGCCGGGGCGGTGTTCCGTACCATTCCCTCCGAGGTGTGGACCATGGA
GGACCTGGGCATGGGCGATGTCTTCCGCCGCCTGTCCATGCTGCCCCGGGGCCTGGTGCTGGTGACGGGTCCCACCGGCT
CGGGCAAGAGCACCACGCTGGCGGCGATGATCGACTACGTCAACGAGAACCGCTTCGAGCACATCCTCACCATCGAGGAC
CCGGTGGAATTCGTCCATCGCAGCAAGCGCTGCCTGGTCAACCAGCGCGAGGTGCATCGCGACACCCACGGCTTCGCCCC
CGCCCTGCGCAGCGCCCTGCGCGAGGACCCCGACGTCATCCTGGTCGGCGAGATGCGCGACCTCGAGACCATCCGGCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGATC
ATCGATGTCTTCCCCGGCGACGAGAAGGCCATGGTGCGTTCGATGCTCTCCGAGTCGCTGCAGGCGGTGATTTCCCAGGC
GCTGCTCAAGCGCCAGGGCGGCGGCCGGGTCGCGGCCCACGAGATCCTCATTGCCACCGCGGCGGTGCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATCTACTCGGCGATCCAGACCGGCGGCAACCTGGGCATGCAGACCCTCGACGCCTCCCTG
GCGCGTCTGGTGGCCGACAACGTGGTGGCCCGGGAAGAGGCCCAGGCCAAGGCCAAGGCCGTCTTGGCGAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

79.941

98.547

0.788
  pilT Pseudomonas stutzeri DSM 10701

79.351

98.547

0.782
  pilT Acinetobacter baumannii D1279779

76.401

98.547

0.753
  pilT Acinetobacter baumannii strain A118

76.401

98.547

0.753
  pilT Acinetobacter nosocomialis M2

76.106

98.547

0.75
  pilT Acinetobacter baylyi ADP1

74.706

98.837

0.738
  pilT Legionella pneumophila strain Lp02

69.822

98.256

0.686
  pilT Legionella pneumophila strain ERS1305867

69.822

98.256

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Neisseria meningitidis 8013

65.588

98.837

0.648
  pilT Neisseria gonorrhoeae MS11

65.294

98.837

0.645
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.917

98.547

0.512
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.384

0.41
  pilU Acinetobacter baylyi ADP1

39.181

99.419

0.39
  pilU Vibrio cholerae strain A1552

39.701

97.384

0.387