Detailed information NTG0737313

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OCT48_RS01060
• Genome accession: NZ_CP107008
• Coordinates: 222538..223680 (+)
• Length: 380 a.a.
• NCBI ID: WP_263590958.1
• Uniprot ID: -
• Organism: Halomonas sp. M4R1S46
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 217538..228680

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OCT48_RS01035 (OCT48_01035)	metX	217997..219154 (-)	1158	WP_263590953.1	homoserine O-succinyltransferase MetX	-
OCT48_RS01040 (OCT48_01040)	-	219192..219785 (-)	594	WP_263590954.1	YggT family protein	-
OCT48_RS01045 (OCT48_01045)	proC	219821..220645 (-)	825	WP_263590955.1	pyrroline-5-carboxylate reductase	-
OCT48_RS01050 (OCT48_01050)	-	220704..221423 (-)	720	WP_263590956.1	YggS family pyridoxal phosphate-dependent enzyme	-
OCT48_RS01055 (OCT48_01055)	pilT	221477..222511 (+)	1035	WP_263590957.1	type IV pilus twitching motility protein PilT	Machinery gene
OCT48_RS01060 (OCT48_01060)	pilU	222538..223680 (+)	1143	WP_263590958.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OCT48_RS01065 (OCT48_01065)	-	223968..225098 (-)	1131	WP_263590959.1	anhydro-N-acetylmuramic acid kinase	-
OCT48_RS01070 (OCT48_01070)	tyrS	225212..226411 (+)	1200	WP_263590960.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42299.37 Da
• Isoelectric Point: 5.9869

>NTDB_id=737313 OCT48_RS01060 WP_263590958.1 222538..223680(+) (pilU) [Halomonas sp. M4R1S46]
MTAKEWLYQLLDIMIEKDASDLLISVGAPPTLKMAGKLTPMGNQGLSNDQVHELVAASMPEAPLQRFIDEHEANFALSLE
GRGRFRVSAFQQRNQPAMVIRRIAFEIPHLEELSLPTVLGELANHKRGLVFVVGGTGTGKSTTLASMIQQRNETIGGHII
SVEDPIEYVHPHRRAIINQREVGIDTDSYEVALKNTLRQAPDVILIGEIRTRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPHERHEQVWLDLSLNLRAIVAQQLLPTMDGGRCPAIEVMLKSPLIGDLIRKAEVSEVKNIMARSRDQGMQTF
DQALYDLFKQGRISEEVALVHADSANDLRMMIKYGDGRGEGLAEAKQAASHLSLRGEDDY

Nucleotide

• Length: 1143 bp

>NTDB_id=737313 OCT48_RS01060 WP_263590958.1 222538..223680(+) (pilU) [Halomonas sp. M4R1S46]
ATGACCGCAAAAGAATGGCTGTACCAGCTGCTGGATATCATGATCGAGAAGGATGCCTCGGACCTGCTGATCTCCGTGGG
CGCACCGCCGACGCTCAAGATGGCCGGCAAGCTGACGCCCATGGGCAATCAGGGGCTGAGCAACGACCAGGTCCACGAAC
TGGTGGCGGCCTCGATGCCGGAAGCGCCTCTCCAGCGCTTCATCGACGAGCACGAGGCCAACTTCGCACTCAGCCTGGAG
GGCCGGGGTCGCTTCCGGGTCAGCGCCTTCCAGCAGCGCAACCAGCCGGCCATGGTGATCCGCCGCATCGCCTTCGAGAT
CCCGCACCTGGAGGAGTTGTCGCTGCCCACGGTGCTCGGTGAGCTGGCCAACCACAAGCGCGGCCTGGTGTTCGTGGTCG
GAGGCACCGGCACCGGCAAGTCGACCACCCTGGCCTCGATGATCCAGCAGCGCAACGAGACCATCGGTGGCCACATCATC
AGCGTCGAGGATCCCATCGAGTACGTGCATCCGCATCGCCGCGCCATCATCAACCAGCGCGAGGTGGGCATCGACACCGA
CTCCTACGAGGTGGCACTGAAGAACACCCTGCGCCAGGCCCCGGACGTCATCCTGATCGGCGAGATCCGCACCCGGGAGA
CCATGGAGCATGCCCTGACCTTCGCCGAGACCGGGCACCTCTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCG
CTCGACCGGATCATCCACTTCTTCCCCCACGAACGGCACGAGCAGGTCTGGCTCGACCTGTCACTGAACCTGCGCGCCAT
CGTCGCCCAGCAGCTGCTGCCCACGATGGATGGCGGGCGCTGCCCGGCCATCGAGGTCATGCTCAAGTCGCCGCTGATCG
GTGACCTGATCCGCAAGGCCGAAGTGTCCGAGGTCAAGAACATCATGGCTCGCTCCCGGGACCAGGGCATGCAGACCTTC
GACCAGGCGCTCTATGACCTCTTCAAGCAGGGGCGCATCTCCGAGGAGGTGGCGCTGGTCCACGCCGATTCGGCCAACGA
CCTGCGCATGATGATCAAGTACGGCGACGGGCGGGGCGAGGGCCTGGCCGAAGCCAAGCAGGCGGCCAGCCACCTCTCGC
TGCGCGGCGAGGACGACTACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (84-274)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.93	98.158	0.608
pilU	Vibrio cholerae strain A1552	59.777	94.211	0.563
pilU	Acinetobacter baylyi ADP1	57.593	91.842	0.529
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.566	90.263	0.384