Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N7L95_RS16245 Genome accession   NZ_CP106951
Coordinates   3669790..3670833 (+) Length   347 a.a.
NCBI ID   WP_301256301.1    Uniprot ID   -
Organism   Eleftheria terrae strain P9846-PB     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3664790..3675833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7L95_RS16230 (N7L95_16255) - 3665389..3668400 (-) 3012 WP_301256298.1 L-glutamate gamma-semialdehyde dehydrogenase -
  N7L95_RS16235 (N7L95_16260) - 3668498..3668980 (+) 483 WP_301256299.1 Lrp/AsnC ligand binding domain-containing protein -
  N7L95_RS16240 (N7L95_16265) - 3669035..3669736 (-) 702 WP_301256300.1 YggS family pyridoxal phosphate-dependent enzyme -
  N7L95_RS16245 (N7L95_16270) pilT 3669790..3670833 (+) 1044 WP_301256301.1 type IV pilus twitching motility protein PilT Machinery gene
  N7L95_RS16250 (N7L95_16275) - 3670864..3671511 (+) 648 WP_301256302.1 cyclic nucleotide-binding domain-containing protein -
  N7L95_RS16255 (N7L95_16280) pilU 3671585..3672721 (+) 1137 WP_301256303.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N7L95_RS16260 (N7L95_16285) - 3672799..3673692 (+) 894 WP_301256304.1 NAD(P)-dependent oxidoreductase -
  N7L95_RS16265 (N7L95_16290) - 3673796..3674464 (-) 669 WP_301256305.1 BON domain-containing protein -
  N7L95_RS16270 (N7L95_16295) - 3674461..3675057 (-) 597 WP_301260166.1 phosphoheptose isomerase -
  N7L95_RS16275 (N7L95_16300) - 3675123..3675494 (-) 372 WP_301256306.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38165.80 Da        Isoelectric Point: 7.0669

>NTDB_id=736798 N7L95_RS16245 WP_301256301.1 3669790..3670833(+) (pilT) [Eleftheria terrae strain P9846-PB]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLEHKQVHDMVYDIMNDSQRKAYEETLECDFSFEIQGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLNAPKIFAELALKPRGLVLVTGPTGSGKSTTLAAMVNHLNESEYGHVLTVED
PIEFVHESKKCLVNQREVGPHTLSFSNALRSALREDPDAILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKDMVRAMLSESVVAIISQTLCKLKDGSGRVAAHEVMLGTSAVRNLIRENKIAQMYSAIQTGNNMGMQTLDQN
LSDLVRRNIISPAEARAKAKFPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=736798 N7L95_RS16245 WP_301256301.1 3669790..3670833(+) (pilT) [Eleftheria terrae strain P9846-PB]
ATGGACATCACCCAGCTGCTGGCTTTTTCGGTCAAGAACAAAGCCTCCGACCTGCACTTGTCGGCCGGGCTGCCGCCGAT
GATCCGTGTCCACGGGGATGTGCGCCGCATCAATGTCGACCCGTTGGAGCACAAGCAAGTGCACGACATGGTGTACGACA
TCATGAACGACTCGCAGCGCAAGGCGTATGAAGAAACGCTGGAGTGCGACTTCTCGTTCGAGATCCAGGGCCTGGCGCGC
TTTCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCCTCGAAGATCCTGACGCTGGA
GCAGCTCAACGCGCCCAAGATCTTCGCCGAGCTGGCGCTCAAGCCGCGCGGCCTGGTGTTGGTGACCGGGCCCACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGGTGAACCACCTCAACGAAAGCGAATACGGCCACGTGCTGACGGTCGAAGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTCGGCCCCCACACGCTGAGCTTCAGCAA
CGCGCTGCGCTCGGCCCTGCGGGAAGATCCGGACGCCATCCTGGTCGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CGCTCACCGCCGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGAGCGCCGCCAAGACCATCGACCGGGTG
GTCGACGTCTTCCCGGCGGCCGAGAAGGACATGGTGCGCGCGATGTTGTCGGAATCGGTCGTCGCCATCATCTCGCAGAC
CCTGTGCAAGCTGAAGGACGGCTCCGGCCGCGTCGCGGCGCACGAGGTCATGCTCGGCACTTCCGCGGTGCGCAACCTGA
TCCGCGAGAACAAGATCGCCCAGATGTACTCGGCCATCCAGACCGGCAACAACATGGGCATGCAGACGCTGGACCAGAAC
CTGTCAGACCTGGTCCGGCGCAACATCATCTCCCCGGCCGAGGCCCGCGCCAAGGCGAAGTTCCCGGAGAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Acinetobacter baylyi ADP1

73.043

99.424

0.726
  pilT Legionella pneumophila strain Lp02

71.976

97.695

0.703
  pilT Legionella pneumophila strain ERS1305867

71.976

97.695

0.703
  pilT Neisseria meningitidis 8013

67.246

99.424

0.669
  pilT Neisseria gonorrhoeae MS11

66.957

99.424

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.976

97.406

0.643
  pilT Vibrio cholerae strain A1552

65.976

97.406

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

99.424

0.496
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398
  pilU Vibrio cholerae strain A1552

40.896

96.542

0.395