Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   N7L95_RS16255 Genome accession   NZ_CP106951
Coordinates   3671585..3672721 (+) Length   378 a.a.
NCBI ID   WP_301256303.1    Uniprot ID   -
Organism   Eleftheria terrae strain P9846-PB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3666585..3677721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7L95_RS16235 (N7L95_16260) - 3668498..3668980 (+) 483 WP_301256299.1 Lrp/AsnC ligand binding domain-containing protein -
  N7L95_RS16240 (N7L95_16265) - 3669035..3669736 (-) 702 WP_301256300.1 YggS family pyridoxal phosphate-dependent enzyme -
  N7L95_RS16245 (N7L95_16270) pilT 3669790..3670833 (+) 1044 WP_301256301.1 type IV pilus twitching motility protein PilT Machinery gene
  N7L95_RS16250 (N7L95_16275) - 3670864..3671511 (+) 648 WP_301256302.1 cyclic nucleotide-binding domain-containing protein -
  N7L95_RS16255 (N7L95_16280) pilU 3671585..3672721 (+) 1137 WP_301256303.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N7L95_RS16260 (N7L95_16285) - 3672799..3673692 (+) 894 WP_301256304.1 NAD(P)-dependent oxidoreductase -
  N7L95_RS16265 (N7L95_16290) - 3673796..3674464 (-) 669 WP_301256305.1 BON domain-containing protein -
  N7L95_RS16270 (N7L95_16295) - 3674461..3675057 (-) 597 WP_301260166.1 phosphoheptose isomerase -
  N7L95_RS16275 (N7L95_16300) - 3675123..3675494 (-) 372 WP_301256306.1 YraN family protein -
  N7L95_RS16280 (N7L95_16305) rsmI 3675493..3676401 (+) 909 WP_301260167.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  N7L95_RS16285 (N7L95_16310) - 3676420..3677469 (-) 1050 WP_301256307.1 septal ring lytic transglycosylase RlpA family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42062.35 Da        Isoelectric Point: 6.5925

>NTDB_id=736799 N7L95_RS16255 WP_301256303.1 3671585..3672721(+) (pilU) [Eleftheria terrae strain P9846-PB]
MERDQASKFINDLLRLMVSRNGSDLFLTAEFPPAIKVDGRVTKVSPQPLTGQHTLALARSIMNDKQAAEFERTKECNFAV
APQGIGRFRVNAFVQQGHVGLVMRVIPQTLPDIDTMGLPQVLKEITMTKRGLVILVGATGSGKSTTLAAMVDYRNENSYG
HIITIEDPVEFVHPHKNCIVTQREVGLDTDGWGQALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKSMISQRLLPRQEGKGRVAAVEIMLNTPLISELIFKGEVAEIKEIMKKSRELG
MQTFDQSLFDLYEGNAVTYEDALRNADSVNDLRLQIKLNSNRARSSDLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=736799 N7L95_RS16255 WP_301256303.1 3671585..3672721(+) (pilU) [Eleftheria terrae strain P9846-PB]
ATGGAAAGAGACCAGGCTTCCAAATTCATCAACGACCTGCTGCGACTGATGGTCAGCCGCAACGGGTCCGACCTCTTCCT
GACCGCCGAATTTCCGCCGGCCATCAAGGTGGACGGGCGCGTGACCAAGGTGTCGCCGCAGCCGCTGACCGGGCAGCACA
CGCTGGCCCTGGCGCGTTCCATCATGAACGACAAGCAGGCGGCCGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGGTG
GCGCCGCAAGGCATCGGGCGCTTCCGCGTCAACGCCTTCGTGCAGCAGGGCCATGTGGGCCTGGTGATGCGGGTGATCCC
GCAGACGCTGCCCGACATCGACACCATGGGCCTGCCCCAGGTGCTCAAGGAAATCACGATGACCAAGCGCGGCCTGGTCA
TCCTGGTGGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGGTGGACTACCGCAACGAGAACTCCTACGGC
CACATCATCACCATCGAGGACCCGGTGGAGTTCGTGCATCCGCACAAGAACTGCATCGTCACCCAGCGCGAGGTGGGCCT
GGACACCGACGGCTGGGGCCAGGCCCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACGATGGAGCACGCGGTGGCCTTCGCCGAGACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCGGAGGAGCGGCGCGCGCAGCTGCTGATGGACTTGTCGCTCAACCT
CAAGTCCATGATCTCGCAGCGGCTGCTGCCGCGCCAGGAGGGCAAGGGCCGGGTGGCCGCGGTCGAGATCATGCTCAACA
CGCCGCTGATCTCCGAGCTGATCTTCAAGGGCGAGGTCGCCGAGATCAAGGAGATCATGAAGAAGAGCCGCGAGCTGGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTCTACGAAGGCAATGCCGTCACCTACGAGGACGCGCTGCGCAATGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCAACCGCGCCCGCAGCTCCGACCTGGCGGCCGGCACCGAGC
ACCTGACCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(99-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.383

96.825

0.585
  pilU Acinetobacter baylyi ADP1

56.131

97.09

0.545
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.478

89.153

0.423
  pilT Pseudomonas aeruginosa PAK

45.994

89.153

0.41
  pilT Pseudomonas stutzeri DSM 10701

44.807

89.153

0.399
  pilT Acinetobacter nosocomialis M2

45.045

88.095

0.397
  pilT Acinetobacter baumannii D1279779

46.395

84.392

0.392
  pilT Acinetobacter baumannii strain A118

46.395

84.392

0.392
  pilT Acinetobacter baylyi ADP1

45.652

85.185

0.389
  pilT Legionella pneumophila strain Lp02

43.544

88.095

0.384
  pilT Legionella pneumophila strain ERS1305867

43.544

88.095

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.969

84.127

0.378
  pilT Vibrio cholerae strain A1552

44.969

84.127

0.378