Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M9799_RS09710 Genome accession   NZ_CP106881
Coordinates   2155938..2156981 (-) Length   347 a.a.
NCBI ID   WP_231041477.1    Uniprot ID   -
Organism   Comamonas endophytica strain 5MLIR     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2150938..2161981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9799_RS09680 (M9799_09680) - 2151314..2151724 (+) 411 WP_231041472.1 YraN family protein -
  M9799_RS09685 (M9799_09685) - 2151774..2152382 (+) 609 WP_231041473.1 SIS domain-containing protein -
  M9799_RS09690 (M9799_09690) - 2152379..2153047 (+) 669 WP_231041474.1 BON domain-containing protein -
  M9799_RS09695 (M9799_09695) - 2153093..2153989 (-) 897 WP_231041475.1 NAD(P)-dependent oxidoreductase -
  M9799_RS09700 (M9799_09700) pilU 2154052..2155188 (-) 1137 WP_231041476.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M9799_RS09705 (M9799_09705) - 2155240..2155863 (-) 624 WP_231042150.1 cyclic nucleotide-binding domain-containing protein -
  M9799_RS09710 (M9799_09710) pilT 2155938..2156981 (-) 1044 WP_231041477.1 type IV pilus twitching motility protein PilT Machinery gene
  M9799_RS09715 (M9799_09715) - 2157020..2157739 (+) 720 WP_231041478.1 YggS family pyridoxal phosphate-dependent enzyme -
  M9799_RS09720 (M9799_09720) - 2157924..2159012 (-) 1089 WP_231041479.1 glycosyl hydrolase family 79 C-terminal domain-containing protein -
  M9799_RS09725 (M9799_09725) - 2159280..2160425 (+) 1146 WP_231041480.1 aminotransferase class V-fold PLP-dependent enzyme -
  M9799_RS09730 (M9799_09730) - 2160449..2160859 (+) 411 WP_231041481.1 FKBP-type peptidyl-prolyl cis-trans isomerase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38332.14 Da        Isoelectric Point: 6.8351

>NTDB_id=736411 M9799_RS09710 WP_231041477.1 2155938..2156981(-) (pilT) [Comamonas endophytica strain 5MLIR]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMIYDIMNDAQRKVYDEFLEIDFSFEIEGLAR
FRVNAFNQYRGAAAVFRTIPSKILTLEQLNAPKIFAELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMTHSFAAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGTGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSQNVGMQTLDQN
LTDLVRRNLISAAEARTKAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=736411 M9799_RS09710 WP_231041477.1 2155938..2156981(-) (pilT) [Comamonas endophytica strain 5MLIR]
GTGGACATCACCCAACTGCTGGCGTTCAGCGTCAAGAACAAGGCCTCCGACCTGCATTTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTGCATGGCGACGTGCGCCGCATCAACGTCGACGCGCTGGACCACAAGACCGTCCACGCCATGATCTACGACA
TCATGAACGACGCCCAGCGCAAGGTCTACGACGAATTCCTCGAGATCGACTTCTCCTTCGAGATCGAAGGCCTGGCGCGC
TTTCGCGTCAACGCCTTCAACCAGTACCGCGGCGCGGCGGCGGTGTTCCGCACCATTCCCAGCAAGATCCTGACGCTCGA
GCAGCTCAACGCACCGAAGATCTTCGCCGAGCTCGCGCTCAAGCCGCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCCATGGTCAACTACCTCAATGAGACCGAGTACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAGTTCGTCCACGACTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGCCCGATGACGCATTCTTTCGCGGC
CGCACTGCGCTCGGCGCTGCGCGAAGACCCCGATGCCATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CGATGACCGCCGCCGAAACCGGCCATCTGGTGTTCGGCACGCTGCACACCTCGAGCGCCGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCGGAGTCGCTGCAGGCGGTCATCTCGCAGAC
GCTTTGCAAGACCAAGGACGGCACGGGCCGCGTGGCGGCGCACGAGATCATGCTCGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTATTCGACCATCCAGACCAGCCAGAACGTCGGCATGCAGACGCTGGATCAGAAC
CTCACCGACCTGGTGCGGCGCAACCTCATCAGCGCCGCCGAAGCCCGCACCAAGGCCAAGATCCCGGACAACTTCCCCGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain ERS1305867

74.336

97.695

0.726
  pilT Legionella pneumophila strain Lp02

74.336

97.695

0.726
  pilT Neisseria meningitidis 8013

68.824

97.983

0.674
  pilT Neisseria gonorrhoeae MS11

68.529

97.983

0.671
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.328

98.271

0.671
  pilT Vibrio cholerae strain A1552

68.328

98.271

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401