Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M9799_RS09700 Genome accession   NZ_CP106881
Coordinates   2154052..2155188 (-) Length   378 a.a.
NCBI ID   WP_231041476.1    Uniprot ID   -
Organism   Comamonas endophytica strain 5MLIR     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2149052..2160188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9799_RS09675 (M9799_09675) rsmI 2150388..2151314 (-) 927 WP_231041471.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  M9799_RS09680 (M9799_09680) - 2151314..2151724 (+) 411 WP_231041472.1 YraN family protein -
  M9799_RS09685 (M9799_09685) - 2151774..2152382 (+) 609 WP_231041473.1 SIS domain-containing protein -
  M9799_RS09690 (M9799_09690) - 2152379..2153047 (+) 669 WP_231041474.1 BON domain-containing protein -
  M9799_RS09695 (M9799_09695) - 2153093..2153989 (-) 897 WP_231041475.1 NAD(P)-dependent oxidoreductase -
  M9799_RS09700 (M9799_09700) pilU 2154052..2155188 (-) 1137 WP_231041476.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M9799_RS09705 (M9799_09705) - 2155240..2155863 (-) 624 WP_231042150.1 cyclic nucleotide-binding domain-containing protein -
  M9799_RS09710 (M9799_09710) pilT 2155938..2156981 (-) 1044 WP_231041477.1 type IV pilus twitching motility protein PilT Machinery gene
  M9799_RS09715 (M9799_09715) - 2157020..2157739 (+) 720 WP_231041478.1 YggS family pyridoxal phosphate-dependent enzyme -
  M9799_RS09720 (M9799_09720) - 2157924..2159012 (-) 1089 WP_231041479.1 glycosyl hydrolase family 79 C-terminal domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42182.61 Da        Isoelectric Point: 6.7722

>NTDB_id=736410 M9799_RS09700 WP_231041476.1 2154052..2155188(-) (pilU) [Comamonas endophytica strain 5MLIR]
MERDQASKFINDLLQLMVSRNGSDLFITADFPPAIKVDGKVTKVSPQPLTPLHTLTLARSIMSDKQIADFERTKESNFAI
SPAGIGRFRVNTFMQQGRIGMVMRTIPMNVPTIDKLGVPQVLKEISLAKRGLCILVGATGSGKSTTLAAMVDWRNENSYG
HIITVEDPIEFVHPHKNCVVTQREVGLDTESWDAALKNTLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRVVNFFPEERRPQLLMDLSLNLRALVSQRLIPKQDGKGRIAAIEVMLNTPLISDLIFKGEVAEIKEVMKKGRELG
MQTFDQALFDLFEAQHITYEDALRNADSVNDLRLQIKLNSQRARNLDLSSGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=736410 M9799_RS09700 WP_231041476.1 2154052..2155188(-) (pilU) [Comamonas endophytica strain 5MLIR]
ATGGAACGAGACCAGGCCAGTAAATTCATCAACGATCTGCTCCAGCTGATGGTCAGCCGCAATGGCAGCGACCTCTTCAT
CACCGCCGACTTCCCGCCGGCCATCAAGGTCGACGGCAAGGTCACCAAGGTTTCGCCCCAGCCGCTGACGCCGCTGCACA
CGCTGACGCTGGCGCGCTCGATCATGAGCGACAAGCAGATCGCCGACTTCGAGCGCACCAAGGAAAGCAACTTCGCGATC
TCGCCGGCGGGCATCGGGCGCTTCCGCGTCAACACCTTCATGCAGCAGGGGCGCATCGGCATGGTCATGCGCACCATCCC
CATGAACGTGCCGACCATCGACAAGCTCGGCGTGCCGCAGGTGCTCAAGGAAATCTCGCTGGCCAAGCGCGGCCTGTGCA
TCCTGGTCGGCGCCACAGGCTCGGGCAAGTCGACCACGCTCGCGGCCATGGTCGACTGGCGCAACGAGAACTCCTACGGC
CACATCATCACCGTCGAGGACCCTATCGAATTCGTGCACCCGCACAAGAACTGCGTGGTCACCCAGCGCGAGGTCGGCCT
CGACACCGAAAGCTGGGACGCGGCGCTAAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAGATACGCG
ACCGCGAGACCATGGAGCACGCGATCGCGTTCTCCGAGACCGGCCACCTGTGCCTGGCGACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGGTGGTCAACTTCTTCCCCGAGGAGCGCCGCCCGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCGCACTGGTGTCGCAGCGCCTGATCCCGAAGCAGGACGGCAAGGGCCGGATCGCGGCCATCGAGGTGATGCTCAACA
CCCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTCGCCGAGATCAAGGAAGTCATGAAAAAGGGCCGCGAGCTGGGC
ATGCAGACCTTCGACCAGGCGTTGTTCGATCTGTTCGAGGCCCAGCACATCACCTACGAGGATGCGTTGCGCAATGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAATCTAGACCTGTCCTCCGGCACAGAGC
ATTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.464

92.328

0.577
  pilU Acinetobacter baylyi ADP1

56.267

94.974

0.534
  pilU Vibrio cholerae strain A1552

52.066

96.032

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

45.697

89.153

0.407
  pilT Pseudomonas stutzeri DSM 10701

44.214

89.153

0.394
  pilT Legionella pneumophila strain ERS1305867

43.109

90.212

0.389
  pilT Legionella pneumophila strain Lp02

43.109

90.212

0.389
  pilT Acinetobacter baumannii strain A118

45.455

84.392

0.384
  pilT Acinetobacter nosocomialis M2

45.455

84.392

0.384
  pilT Acinetobacter baumannii D1279779

45.455

84.392

0.384
  pilT Acinetobacter baylyi ADP1

43.963

85.45

0.376