Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N8I74_RS15490 Genome accession   NZ_CP106753
Coordinates   3365716..3366759 (-) Length   347 a.a.
NCBI ID   WP_263124011.1    Uniprot ID   -
Organism   Chitiniphilus purpureus strain CD1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3360716..3371759
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8I74_RS15475 (N8I74_15475) - 3360741..3362075 (-) 1335 WP_263124008.1 pre-peptidase C-terminal domain-containing protein -
  N8I74_RS15480 (N8I74_15480) - 3362209..3364443 (-) 2235 WP_263124009.1 FimV family protein -
  N8I74_RS15485 (N8I74_15485) pilU 3364490..3365626 (-) 1137 WP_263124010.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N8I74_RS15490 (N8I74_15490) pilT 3365716..3366759 (-) 1044 WP_263124011.1 type IV pilus twitching motility protein PilT Machinery gene
  N8I74_RS15495 (N8I74_15495) - 3366855..3367568 (+) 714 WP_263124012.1 YggS family pyridoxal phosphate-dependent enzyme -
  N8I74_RS15500 (N8I74_15500) proC 3367572..3368381 (+) 810 WP_263124013.1 pyrroline-5-carboxylate reductase -
  N8I74_RS15505 (N8I74_15505) - 3368551..3369138 (+) 588 WP_263124014.1 YggT family protein -
  N8I74_RS15510 (N8I74_15510) - 3369138..3369428 (+) 291 WP_263124015.1 DUF167 domain-containing protein -
  N8I74_RS15515 (N8I74_15515) - 3369418..3369822 (-) 405 WP_263124016.1 FxsA family protein -
  N8I74_RS15520 (N8I74_15520) cutA 3369821..3370159 (+) 339 WP_263124017.1 divalent-cation tolerance protein CutA -
  N8I74_RS15525 (N8I74_15525) dsbD 3370156..3371694 (+) 1539 WP_263124018.1 protein-disulfide reductase DsbD -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38255.96 Da        Isoelectric Point: 6.6672

>NTDB_id=734481 N8I74_RS15490 WP_263124011.1 3365716..3366759(-) (pilT) [Chitiniphilus purpureus strain CD1]
MEISELLAFAVKNKASDLHLSSGLPPMIRVHGDVRRINLPALEHKDVHDMVYDIMNDGQRKVYEDTLEADFSFEIPNLAR
FRVNAFVQNRGAGAVFRTIPSKVLTLEELNAPRVFQDIAENPRGLVLVTGPTGSGKSTTLAAMINYINENEYGHILTVED
PIEFVHQSKKCLINQREVGPHTLSFANALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRAMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGVPAIRNLIRENKIAQMYSAIQTGQQFGMNTLDQA
LLTLVQRNVVSLGEARAKAAIPDNFKG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=734481 N8I74_RS15490 WP_263124011.1 3365716..3366759(-) (pilT) [Chitiniphilus purpureus strain CD1]
ATGGAAATTTCCGAACTCTTGGCTTTTGCCGTCAAGAACAAGGCCTCCGATCTGCACCTGTCCTCCGGACTGCCGCCGAT
GATCCGCGTGCACGGCGATGTGCGGCGCATCAATCTGCCCGCGCTCGAGCACAAGGACGTGCACGACATGGTGTACGACA
TCATGAACGACGGGCAGCGCAAGGTTTATGAGGATACGCTGGAGGCGGACTTCTCGTTCGAGATCCCCAACCTTGCACGT
TTCCGCGTCAACGCCTTCGTGCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTACTGACGCTGGA
GGAGCTGAATGCGCCGCGGGTGTTCCAGGACATCGCCGAGAACCCGCGCGGGCTTGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCGGCCATGATCAATTACATCAACGAGAACGAGTACGGGCACATCCTGACGGTGGAGGAC
CCGATCGAATTCGTGCACCAGTCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGCCCGCATACGCTGAGCTTTGCCAA
TGCGCTGCGCTCCGCGCTGCGTGAGGACCCGGACGTGATCCTGGTCGGCGAAATGCGTGACCTGGAGACCATCCGGCTTG
CACTGACTGCCGCCGAGACCGGTCACCTGGTGTTCGGCACGCTGCATACCAGTTCGGCCGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCCGCGGCGGAAAAGGAGATGGTGCGCGCCATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGAC
GCTGCTCAAGACCAAGGACGGGCAGGGCCGCGTGGCGGCGCACGAGATCATGATCGGCGTGCCGGCCATCCGCAACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTATTCGGCGATCCAGACCGGGCAGCAGTTCGGCATGAACACGCTGGACCAGGCG
CTCTTGACGCTGGTGCAGCGCAACGTGGTGTCGCTTGGCGAGGCACGGGCCAAGGCCGCGATCCCGGACAATTTCAAGGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.13

99.424

0.787
  pilT Acinetobacter baumannii D1279779

79.13

99.424

0.787
  pilT Acinetobacter baumannii strain A118

79.13

99.424

0.787
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Neisseria meningitidis 8013

73.746

97.695

0.72
  pilT Neisseria gonorrhoeae MS11

73.451

97.695

0.718
  pilT Legionella pneumophila strain Lp02

73.414

95.389

0.7
  pilT Legionella pneumophila strain ERS1305867

73.414

95.389

0.7
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.751

97.406

0.66
  pilT Vibrio cholerae strain A1552

67.751

97.406

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

99.424

0.516
  pilU Vibrio cholerae strain A1552

43.284

96.542

0.418
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395
  pilU Acinetobacter baylyi ADP1

38.905

100

0.389