Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   N8I74_RS15485 Genome accession   NZ_CP106753
Coordinates   3364490..3365626 (-) Length   378 a.a.
NCBI ID   WP_263124010.1    Uniprot ID   -
Organism   Chitiniphilus purpureus strain CD1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3359490..3370626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8I74_RS15475 (N8I74_15475) - 3360741..3362075 (-) 1335 WP_263124008.1 pre-peptidase C-terminal domain-containing protein -
  N8I74_RS15480 (N8I74_15480) - 3362209..3364443 (-) 2235 WP_263124009.1 FimV family protein -
  N8I74_RS15485 (N8I74_15485) pilU 3364490..3365626 (-) 1137 WP_263124010.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N8I74_RS15490 (N8I74_15490) pilT 3365716..3366759 (-) 1044 WP_263124011.1 type IV pilus twitching motility protein PilT Machinery gene
  N8I74_RS15495 (N8I74_15495) - 3366855..3367568 (+) 714 WP_263124012.1 YggS family pyridoxal phosphate-dependent enzyme -
  N8I74_RS15500 (N8I74_15500) proC 3367572..3368381 (+) 810 WP_263124013.1 pyrroline-5-carboxylate reductase -
  N8I74_RS15505 (N8I74_15505) - 3368551..3369138 (+) 588 WP_263124014.1 YggT family protein -
  N8I74_RS15510 (N8I74_15510) - 3369138..3369428 (+) 291 WP_263124015.1 DUF167 domain-containing protein -
  N8I74_RS15515 (N8I74_15515) - 3369418..3369822 (-) 405 WP_263124016.1 FxsA family protein -
  N8I74_RS15520 (N8I74_15520) cutA 3369821..3370159 (+) 339 WP_263124017.1 divalent-cation tolerance protein CutA -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42288.42 Da        Isoelectric Point: 6.4347

>NTDB_id=734480 N8I74_RS15485 WP_263124010.1 3364490..3365626(-) (pilU) [Chitiniphilus purpureus strain CD1]
MEKEQAAKFMQDLLRHMRSKSASDLFITADFPPAMKIDGRVTPVSAQTLTAQHTKELARSIMNDRQAEEFEATKECNFAI
NPGSLGRFRVNTFMQQGRVGMVLRTINSEIPKLAELGLPPVLQDIAMTKRGLVIFVGGTGSGKSTSLAAMIGHRNEASYD
HIITIEDPIEYVHEHRNCIVTQREIGVDTDSWGAALRNTLRQAPDVILIGEIRDRDTMDYAIAFAETGHLCMATLHANSS
NQALDRIINFFPEERRQQLLMDLSLNLKAFVSQRLVPHRSGRGRVAAVEVMLNSPLISDLVFKGEVHEIKEIMKKSRELG
MQTFDQSLFDLYEAGKISYEDALRNADSVNDLRLQIKLQGAESKQKDVLAGIDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=734480 N8I74_RS15485 WP_263124010.1 3364490..3365626(-) (pilU) [Chitiniphilus purpureus strain CD1]
ATGGAAAAAGAGCAAGCAGCCAAGTTCATGCAGGATCTCCTGCGTCACATGCGTTCCAAGAGCGCCTCCGACCTTTTCAT
CACCGCCGACTTCCCGCCGGCGATGAAGATCGACGGCAGGGTGACCCCGGTATCGGCCCAGACCTTGACGGCGCAGCACA
CCAAGGAGCTTGCACGTTCGATCATGAACGACCGCCAGGCCGAGGAGTTCGAGGCGACCAAGGAGTGCAACTTCGCCATC
AACCCCGGCTCGCTGGGGCGCTTTCGCGTCAATACCTTCATGCAGCAAGGCCGGGTCGGCATGGTGCTGCGGACGATCAA
TTCGGAGATCCCCAAGCTTGCCGAGCTGGGCCTGCCGCCGGTGCTGCAGGATATCGCGATGACCAAGCGCGGGCTGGTGA
TCTTCGTCGGTGGCACTGGCAGCGGCAAGTCCACCTCGCTTGCCGCCATGATCGGACACCGCAACGAAGCGTCGTATGAC
CACATCATCACCATCGAGGACCCGATCGAGTACGTGCACGAGCACAGGAACTGCATCGTCACGCAGCGCGAGATCGGCGT
GGACACCGATTCGTGGGGTGCTGCACTGCGCAATACGCTGCGTCAGGCGCCGGATGTCATCCTGATCGGTGAGATCCGCG
ACCGCGACACGATGGACTATGCGATCGCGTTCGCCGAGACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACTCGTCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAGGAGCGCCGTCAGCAGCTGCTGATGGATCTGTCGCTCAACCT
CAAGGCATTCGTTTCGCAGCGGCTGGTGCCGCATCGCTCCGGCCGCGGCCGGGTGGCCGCGGTCGAGGTCATGCTCAACT
CGCCGCTGATTTCCGATCTGGTCTTCAAGGGCGAGGTGCACGAGATCAAGGAGATCATGAAGAAATCCCGAGAGCTTGGC
ATGCAGACCTTCGATCAGAGCCTGTTCGACCTCTATGAGGCGGGCAAGATCAGCTACGAGGACGCGTTGCGCAACGCCGA
CTCGGTGAACGACCTGCGTCTGCAGATCAAGCTGCAAGGCGCGGAATCGAAACAGAAGGATGTGCTTGCGGGCATCGATC
ATCTGGATATCGTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(19-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.864

93.651

0.579
  pilU Acinetobacter baylyi ADP1

59.664

94.444

0.563
  pilU Vibrio cholerae strain A1552

54.545

90.212

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

89.153

0.399
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373
  pilT Legionella pneumophila strain Lp02

42.042

88.095

0.37
  pilT Legionella pneumophila strain ERS1305867

42.042

88.095

0.37
  pilT Pseudomonas aeruginosa PAK

41.246

89.153

0.368
  pilT Pseudomonas stutzeri DSM 10701

40.653

89.153

0.362
  pilT Acinetobacter nosocomialis M2

41.141

88.095

0.362
  pilT Acinetobacter baumannii D1279779

41.141

88.095

0.362
  pilT Acinetobacter baumannii strain A118

41.141

88.095

0.362