Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N7220_RS13935 Genome accession   NZ_CP106736
Coordinates   3040989..3042032 (+) Length   347 a.a.
NCBI ID   WP_283148133.1    Uniprot ID   -
Organism   Silvimonas soli strain 17-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3035989..3047032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7220_RS13900 cutA 3037012..3037335 (-) 324 WP_283148126.1 divalent-cation tolerance protein CutA -
  N7220_RS13905 - 3037362..3037775 (+) 414 WP_283148127.1 FxsA family protein -
  N7220_RS13910 - 3037790..3038077 (-) 288 WP_283148128.1 DUF167 family protein -
  N7220_RS13915 - 3038074..3038661 (-) 588 WP_283148129.1 YggT family protein -
  N7220_RS13920 proC 3038676..3039494 (-) 819 WP_283148130.1 pyrroline-5-carboxylate reductase -
  N7220_RS13925 - 3039579..3040283 (-) 705 WP_283148131.1 YggS family pyridoxal phosphate-dependent enzyme -
  N7220_RS13930 - 3040418..3040618 (-) 201 WP_283148132.1 hypothetical protein -
  N7220_RS13935 pilT 3040989..3042032 (+) 1044 WP_283148133.1 type IV pilus twitching motility protein PilT Machinery gene
  N7220_RS13940 pilU 3042243..3043379 (+) 1137 WP_283148134.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N7220_RS13945 - 3043421..3045556 (+) 2136 WP_283148135.1 FimV family protein -
  N7220_RS13950 - 3045596..3046522 (-) 927 WP_283148136.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38556.26 Da        Isoelectric Point: 6.8317

>NTDB_id=734212 N7220_RS13935 WP_283148133.1 3040989..3042032(+) (pilT) [Silvimonas soli strain 17-6]
MEISELLAFAVKNKASDLHLSSGLPPMIRVHGDVRRINLPPLEHKDVHDMVYDIMNDGQRKIYEDTLECDFSFEIPNLAR
FRVNAFVQNRGAGAVFRTIPSKVLTLEELNTPKIFRDISENPRGLVLVTGPTGSGKSTTLAAMVNYINENEYGHILTIED
PIEFVHQSKKCLINQREVGPHTLSFSNALRSALREDPDVVLVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRAMLSESLRAVISQTLLKTKDGQSRVAAHEIMIGIPAIRNLIRENKIAQMYSSIQTGQQFGMQTLDQS
LQNLVQRNICSVAEARTKAAIPDNFKG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=734212 N7220_RS13935 WP_283148133.1 3040989..3042032(+) (pilT) [Silvimonas soli strain 17-6]
ATGGAAATATCAGAACTGCTCGCGTTCGCCGTTAAAAACAAAGCGTCGGACTTGCACCTCTCCAGTGGCTTGCCTCCCAT
GATCCGCGTCCACGGTGACGTGCGCCGCATCAACTTGCCGCCGCTGGAACACAAAGACGTGCATGACATGGTGTACGACA
TCATGAACGACGGGCAGCGCAAGATTTACGAAGACACACTGGAATGTGACTTCTCGTTCGAGATTCCCAATCTGGCGCGT
TTCCGGGTCAATGCTTTTGTGCAAAACCGCGGTGCGGGTGCGGTGTTCCGGACCATTCCGTCCAAAGTGCTGACGCTGGA
AGAACTGAACACCCCCAAGATTTTCCGCGATATTTCCGAAAACCCGCGTGGCCTGGTGTTGGTAACCGGCCCAACCGGCT
CGGGTAAGTCCACCACGCTGGCGGCGATGGTGAACTACATCAACGAGAACGAATATGGTCACATCCTGACCATTGAAGAT
CCGATTGAATTTGTGCACCAGTCCAAGAAATGCCTGATCAACCAGCGTGAAGTCGGCCCGCATACGCTGAGCTTCTCCAA
CGCGCTGCGTTCAGCGCTGCGGGAAGATCCGGACGTGGTGCTGGTGGGCGAAATGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGTCACCTGGTGTTTGGCACATTGCACACCAGTTCTGCAGCCAAAACGATTGACCGGGTG
GTCGATGTTTTCCCGGCAGCAGAAAAAGAAATGGTGCGGGCGATGTTGTCTGAATCGCTGCGCGCGGTGATCTCGCAAAC
ACTGCTGAAAACCAAAGACGGCCAGTCCCGGGTGGCTGCGCACGAAATCATGATCGGTATTCCGGCGATTCGTAACTTGA
TCCGTGAAAACAAGATTGCGCAGATGTATTCGTCGATTCAGACCGGTCAGCAATTTGGTATGCAAACGCTGGATCAATCG
CTGCAGAACCTGGTTCAGCGCAATATCTGTTCGGTGGCAGAGGCGCGCACCAAGGCGGCTATTCCGGATAACTTCAAGGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.551

99.424

0.781
  pilT Acinetobacter baumannii D1279779

78.551

99.424

0.781
  pilT Acinetobacter baumannii strain A118

78.551

99.424

0.781
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Neisseria meningitidis 8013

73.451

97.695

0.718
  pilT Neisseria gonorrhoeae MS11

73.156

97.695

0.715
  pilT Legionella pneumophila strain Lp02

73.414

95.389

0.7
  pilT Legionella pneumophila strain ERS1305867

73.414

95.389

0.7
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.864

97.406

0.651
  pilT Vibrio cholerae strain A1552

66.864

97.406

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Acinetobacter baylyi ADP1

39.481

100

0.395
  pilU Pseudomonas stutzeri DSM 10701

41.667

93.372

0.389
  pilU Vibrio cholerae strain A1552

41.538

93.66

0.389