Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   N7220_RS13940 Genome accession   NZ_CP106736
Coordinates   3042243..3043379 (+) Length   378 a.a.
NCBI ID   WP_283148134.1    Uniprot ID   -
Organism   Silvimonas soli strain 17-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3037243..3048379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7220_RS13905 - 3037362..3037775 (+) 414 WP_283148127.1 FxsA family protein -
  N7220_RS13910 - 3037790..3038077 (-) 288 WP_283148128.1 DUF167 family protein -
  N7220_RS13915 - 3038074..3038661 (-) 588 WP_283148129.1 YggT family protein -
  N7220_RS13920 proC 3038676..3039494 (-) 819 WP_283148130.1 pyrroline-5-carboxylate reductase -
  N7220_RS13925 - 3039579..3040283 (-) 705 WP_283148131.1 YggS family pyridoxal phosphate-dependent enzyme -
  N7220_RS13930 - 3040418..3040618 (-) 201 WP_283148132.1 hypothetical protein -
  N7220_RS13935 pilT 3040989..3042032 (+) 1044 WP_283148133.1 type IV pilus twitching motility protein PilT Machinery gene
  N7220_RS13940 pilU 3042243..3043379 (+) 1137 WP_283148134.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N7220_RS13945 - 3043421..3045556 (+) 2136 WP_283148135.1 FimV family protein -
  N7220_RS13950 - 3045596..3046522 (-) 927 WP_283148136.1 LysR substrate-binding domain-containing protein -
  N7220_RS13955 - 3046672..3047283 (+) 612 WP_283148137.1 FMN-dependent NADH-azoreductase -
  N7220_RS13960 - 3047354..3047953 (+) 600 WP_283148138.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42249.44 Da        Isoelectric Point: 6.2734

>NTDB_id=734213 N7220_RS13940 WP_283148134.1 3042243..3043379(+) (pilU) [Silvimonas soli strain 17-6]
MEKEQAAKFMQDLLRHMRAKGASDLFITVDFPPAMKVDGRVTPVSNQVLSSQHTKELARAIMNDRQAEEFEATHECNFAI
SPGTLGRYRVNAFMQQGRVGMVLRTINSHIPKLDELGLPMVLHDIAMAKRGLVIFVGGTGSGKSTSLAAMIGERNEKAYD
HIITIEDPIEYVHEHKNCIVTQREVGVDTDSWGSALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHANSS
NQALDRIINFFPEERRSQLLMDLSLNLKAFVSQRLVPHRSGQGRVAAVEVMLNSPLISELIFKGEVHEIKEIMKKSRELG
MQTFDQSLFDLYEAGLITYEDALRNADSVNDLRLSIKLQGAEAKHKDVLSGIDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=734213 N7220_RS13940 WP_283148134.1 3042243..3043379(+) (pilU) [Silvimonas soli strain 17-6]
ATGGAAAAAGAACAAGCAGCCAAGTTCATGCAGGATCTCTTGCGCCATATGCGGGCCAAGGGTGCATCGGACTTGTTTAT
TACGGTTGATTTTCCACCGGCAATGAAGGTGGATGGCCGGGTTACGCCGGTTTCCAATCAGGTTCTTTCTTCGCAGCACA
CCAAAGAACTGGCGCGCGCGATCATGAATGACCGGCAGGCAGAAGAATTCGAAGCCACGCATGAATGTAATTTTGCGATC
AGCCCGGGCACGCTCGGGCGTTATCGGGTTAATGCGTTCATGCAGCAAGGTCGCGTGGGCATGGTCTTGCGGACCATCAA
TTCGCATATCCCCAAGCTGGATGAACTCGGCCTGCCCATGGTCTTGCACGATATCGCCATGGCCAAGCGCGGTCTGGTGA
TCTTTGTTGGCGGTACCGGTTCAGGTAAATCGACCTCGCTGGCCGCGATGATTGGTGAGCGTAACGAGAAAGCCTACGAT
CACATCATTACCATCGAAGACCCGATTGAATACGTGCACGAGCATAAAAATTGTATCGTCACGCAACGCGAAGTAGGGGT
TGATACAGACTCTTGGGGCTCGGCGCTGAAGAATACTTTGCGTCAGGCGCCTGACGTGATTCTGATCGGGGAAATCCGTG
ATCGTGAAACCATGGATTACGCCATTGCTTTTGCCGAAACCGGTCACTTGTGTATGGCTACTTTGCATGCCAACTCCTCC
AATCAGGCATTGGACCGGATTATCAACTTCTTCCCGGAAGAACGCCGTTCGCAATTGCTGATGGATTTGTCGTTAAATCT
GAAGGCATTTGTATCGCAACGTCTTGTTCCGCACCGCTCCGGCCAGGGACGCGTAGCTGCAGTTGAGGTTATGCTTAATT
CACCGCTGATTTCTGAACTGATCTTCAAGGGCGAAGTGCACGAGATCAAGGAAATCATGAAGAAGTCGCGCGAATTGGGG
ATGCAGACCTTTGACCAGTCTTTGTTTGATCTTTATGAAGCGGGCCTGATTACCTACGAAGATGCATTACGTAATGCCGA
TTCGGTCAACGATTTGCGACTTAGCATCAAGTTGCAAGGTGCAGAAGCCAAACATAAAGATGTGCTTTCCGGAATCGACC
ATCTGGATATCGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.559

93.651

0.595
  pilU Acinetobacter baylyi ADP1

59.483

92.063

0.548
  pilU Vibrio cholerae strain A1552

54.857

92.593

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas stutzeri DSM 10701

42.433

89.153

0.378
  pilT Pseudomonas aeruginosa PAK

42.433

89.153

0.378
  pilT Acinetobacter baylyi ADP1

43.925

84.921

0.373
  pilT Acinetobacter baumannii strain A118

42.042

88.095

0.37
  pilT Acinetobacter baumannii D1279779

42.042

88.095

0.37
  pilT Legionella pneumophila strain Lp02

42.042

88.095

0.37
  pilT Legionella pneumophila strain ERS1305867

42.042

88.095

0.37
  pilT Acinetobacter nosocomialis M2

42.042

88.095

0.37