Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N7V09_RS17215 Genome accession   NZ_CP104900
Coordinates   3839840..3840877 (-) Length   345 a.a.
NCBI ID   WP_011716282.1    Uniprot ID   A0A2M7HST3
Organism   Shewanella seohaensis strain KCTC 23556     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3834840..3845877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7V09_RS17180 (N7V09_17180) - 3835013..3835327 (+) 315 WP_011716287.1 hypothetical protein -
  N7V09_RS17185 (N7V09_17185) yciH 3835476..3835805 (+) 330 WP_011625632.1 stress response translation initiation inhibitor YciH -
  N7V09_RS17190 (N7V09_17190) - 3835874..3836437 (+) 564 WP_011621987.1 YqgE/AlgH family protein -
  N7V09_RS17195 (N7V09_17195) ruvX 3836468..3836893 (+) 426 WP_011621986.1 Holliday junction resolvase RuvX -
  N7V09_RS17200 (N7V09_17200) hemH 3836955..3837938 (-) 984 WP_248967315.1 ferrochelatase -
  N7V09_RS17205 (N7V09_17205) - 3838008..3838541 (-) 534 WP_248967410.1 glutathione peroxidase -
  N7V09_RS17210 (N7V09_17210) pilU 3838718..3839830 (-) 1113 WP_011621983.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N7V09_RS17215 (N7V09_17215) pilT 3839840..3840877 (-) 1038 WP_011716282.1 type IV pilus twitching motility protein PilT Machinery gene
  N7V09_RS17220 (N7V09_17220) - 3840913..3841629 (+) 717 WP_248967316.1 YggS family pyridoxal phosphate-dependent enzyme -
  N7V09_RS17225 (N7V09_17225) proC 3841854..3842672 (+) 819 WP_086904529.1 pyrroline-5-carboxylate reductase -
  N7V09_RS17230 (N7V09_17230) - 3842748..3843296 (+) 549 WP_248967317.1 YggT family protein -
  N7V09_RS17235 (N7V09_17235) yggU 3843296..3843586 (+) 291 WP_011621978.1 DUF167 family protein YggU -
  N7V09_RS17240 (N7V09_17240) - 3843655..3844089 (+) 435 WP_011621977.1 DUF4426 domain-containing protein -
  N7V09_RS17245 (N7V09_17245) rdgB 3844250..3844866 (+) 617 Protein_3312 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38356.02 Da        Isoelectric Point: 6.9097

>NTDB_id=733245 N7V09_RS17215 WP_011716282.1 3839840..3840877(-) (pilT) [Shewanella seohaensis strain KCTC 23556]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMSKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=733245 N7V09_RS17215 WP_011716282.1 3839840..3840877(-) (pilT) [Shewanella seohaensis strain KCTC 23556]
ATGGAAATCACTGAGTTATTAGCCTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCTAT
GATCCGTGTCGACGGTGAAGTGAGAAAAATTAACCTGCCTGCGCTCGATCATCAGGGTGTACACAGCCTTGTGTACGACA
TTATGAATGATAAACAGCGTAAGGACTTTGAAGAGCATTTAGAAATCGACTTTTCGTTCGAAGTCCCTAATTTAGCGCGT
TTCCGTGTGAACGCTTTTAACCAATCCCGCGGCGCGGCGGCGGTGTTTCGTACCATTCCTAGCGAAATTTTGTCGCTTGA
GCAGTTAGGGGCGCCAGAGATTTTTAAGAAAATTTCCAGCTTTCCCCGCGGCTTAGTGCTTGTTACTGGGCCTACCGGTT
CGGGTAAGAGTACCACACTTGCGGCCATGGTGGATTACATCAATGAGAACCGCCACGACCATATCTTAACCATTGAAGAT
CCTATCGAGTTCGTTCACCAGAATAAGCAATGTTTGATTAACCAACGGGAAGTGCATCGTCATACCCACAGCTTTAACGC
GGCGCTGCGTAGCGCACTGCGTGAAGACCCTGACGTTATCCTCGTCGGTGAGATGCGCGACCTTGAAACCATTCGTCTGG
CGATGACTGCGGCCGAAACGGGTCACTTAGTCTTTGGTACCTTGCACACCACCTCGGCGGCTAAGACCATCGACCGTGTG
GTTGACGTTTTCCCTGCTGGTGAAAAGGACATGGTGCGTACCATGTTGTCTGAATCATTACAGGCGGTTATTTCGCAAAC
CCTGATTAAGAAAATTGGTGGTGGCCGTGTGGCTGCCCACGAAATCATGATGGGTACGCCCGCTATTCGTAACCTTATCC
GTGAAGATAAAGTGGCGCAGATGTACTCAGCCATTCAAACGGGAATGGCCCATGGCATGCAAACGCTCGAACAGTGTCTG
CAAAACTTAGTGAACCGTGGCCTCATCACCCGTGAGGATGCCATGTCGAAGAGTTCAAACAAACAAGCGACGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2M7HST3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.592

99.42

0.791
  pilT Acinetobacter baumannii strain A118

79.592

99.42

0.791
  pilT Acinetobacter nosocomialis M2

79.592

99.42

0.791
  pilT Pseudomonas aeruginosa PAK

79.586

97.971

0.78
  pilT Acinetobacter baylyi ADP1

80.547

95.362

0.768
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.853

98.261

0.49
  pilU Pseudomonas stutzeri DSM 10701

41.691

99.42

0.414
  pilU Vibrio cholerae strain A1552

41.369

97.391

0.403
  pilU Acinetobacter baylyi ADP1

39.763

97.681

0.388