Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N0O74_RS04780 Genome accession   NZ_CP104863
Coordinates   1083173..1083604 (+) Length   143 a.a.
NCBI ID   WP_076739180.1    Uniprot ID   A0A854F7L4
Organism   Stenotrophomonas maltophilia strain CW002SM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1078173..1088604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N0O74_RS04760 (N0O74_04760) - 1079483..1079869 (+) 387 WP_076739183.1 hypothetical protein -
  N0O74_RS04765 (N0O74_04765) coaE 1080062..1080673 (-) 612 WP_171969077.1 dephospho-CoA kinase -
  N0O74_RS04770 (N0O74_04770) - 1080685..1081548 (-) 864 WP_005410800.1 A24 family peptidase -
  N0O74_RS04775 (N0O74_04775) pilC 1081556..1082815 (-) 1260 WP_076739181.1 type II secretion system F family protein Machinery gene
  N0O74_RS04780 (N0O74_04780) pilA 1083173..1083604 (+) 432 WP_076739180.1 pilin Machinery gene
  N0O74_RS04785 (N0O74_04785) - 1083713..1084135 (+) 423 WP_076739179.1 pilin -
  N0O74_RS04790 (N0O74_04790) pilB 1084201..1085934 (+) 1734 WP_076739178.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N0O74_RS04795 (N0O74_04795) pilR 1086059..1087432 (-) 1374 WP_005410795.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14708.90 Da        Isoelectric Point: 8.0996

>NTDB_id=732832 N0O74_RS04780 WP_076739180.1 1083173..1083604(+) (pilA) [Stenotrophomonas maltophilia strain CW002SM]
MNNQKGFTLIELMIVVAIIAILAAIAIPQYNDYTARAQMTEAYTLAAGLKTPIAEAFAQDNGSGSCAKPANAVDKGKYVA
SVTATGDATKCTITATMAGTGVNDKVKSKKVELAFASGTGAWTCTTDAPSEVQPKACTTALKP

Nucleotide


Download         Length: 432 bp        

>NTDB_id=732832 N0O74_RS04780 WP_076739180.1 1083173..1083604(+) (pilA) [Stenotrophomonas maltophilia strain CW002SM]
ATGAATAACCAGAAGGGCTTCACCCTGATCGAACTGATGATCGTCGTTGCGATCATCGCGATTCTGGCCGCCATTGCCAT
CCCGCAGTACAACGACTATACCGCTCGCGCCCAGATGACCGAAGCCTACACCCTGGCTGCCGGCCTGAAGACCCCGATCG
CGGAAGCGTTCGCCCAGGACAATGGCAGCGGCTCATGTGCTAAGCCGGCTAATGCCGTTGACAAGGGTAAGTACGTTGCT
AGTGTTACTGCCACCGGTGATGCTACCAAGTGCACCATCACTGCGACGATGGCGGGAACTGGCGTTAACGACAAGGTCAA
GAGCAAGAAGGTTGAGTTGGCCTTTGCTTCTGGCACTGGCGCTTGGACCTGCACCACCGATGCCCCGTCGGAAGTGCAGC
CGAAGGCGTGCACCACGGCTTTGAAGCCGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A854F7L4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter nosocomialis M2

48.611

100

0.49
  pilA Acinetobacter baumannii strain A118

45.578

100

0.469
  pilA Pseudomonas aeruginosa PAK

37.725

100

0.441
  pilA/pilA1 Eikenella corrodens VA1

41.611

100

0.434
  pilE Neisseria gonorrhoeae strain FA1090

46.512

90.21

0.42
  pilA2 Legionella pneumophila str. Paris

46.154

90.909

0.42
  pilA Vibrio cholerae C6706

40.816

100

0.42
  pilA Vibrio cholerae strain A1552

40.816

100

0.42
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.816

100

0.42
  comP Acinetobacter baylyi ADP1

40.136

100

0.413
  pilE Neisseria gonorrhoeae MS11

45.736

90.21

0.413
  pilA2 Legionella pneumophila strain ERS1305867

44.186

90.21

0.399
  pilA Vibrio campbellii strain DS40M4

37.241

100

0.378
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.143

97.902

0.364