Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N0O74_RS04790 Genome accession   NZ_CP104863
Coordinates   1084201..1085934 (+) Length   577 a.a.
NCBI ID   WP_076739178.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain CW002SM     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1079201..1090934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N0O74_RS04760 (N0O74_04760) - 1079483..1079869 (+) 387 WP_076739183.1 hypothetical protein -
  N0O74_RS04765 (N0O74_04765) coaE 1080062..1080673 (-) 612 WP_171969077.1 dephospho-CoA kinase -
  N0O74_RS04770 (N0O74_04770) - 1080685..1081548 (-) 864 WP_005410800.1 A24 family peptidase -
  N0O74_RS04775 (N0O74_04775) pilC 1081556..1082815 (-) 1260 WP_076739181.1 type II secretion system F family protein Machinery gene
  N0O74_RS04780 (N0O74_04780) pilA 1083173..1083604 (+) 432 WP_076739180.1 pilin Machinery gene
  N0O74_RS04785 (N0O74_04785) - 1083713..1084135 (+) 423 WP_076739179.1 pilin -
  N0O74_RS04790 (N0O74_04790) pilB 1084201..1085934 (+) 1734 WP_076739178.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N0O74_RS04795 (N0O74_04795) pilR 1086059..1087432 (-) 1374 WP_005410795.1 sigma-54 dependent transcriptional regulator Regulator
  N0O74_RS04800 (N0O74_04800) - 1087493..1089106 (-) 1614 WP_049403027.1 ATP-binding protein -
  N0O74_RS04805 (N0O74_04805) sucC 1089464..1090633 (+) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62408.41 Da        Isoelectric Point: 5.8722

>NTDB_id=732833 N0O74_RS04790 WP_076739178.1 1084201..1085934(+) (pilB) [Stenotrophomonas maltophilia strain CW002SM]
MNTVVTANLVGITGLARRLVQDGFLDEATARDAMAKAAAARQPLPTWFAQNKLVTASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGMGNLDVAAGDEDLGATGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPSSLPE
HALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGVYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKK
AAAGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=732833 N0O74_RS04790 WP_076739178.1 1084201..1085934(+) (pilB) [Stenotrophomonas maltophilia strain CW002SM]
ATGAACACAGTTGTCACCGCCAATCTTGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTGCAGGACGGGTTCCTGGACGA
GGCCACCGCCCGCGACGCCATGGCCAAGGCCGCCGCCGCGCGCCAGCCGCTGCCGACGTGGTTCGCGCAGAACAAGCTGG
TCACTGCCTCGCAGCTGGCCGCCGCCAATGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACCTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGTTGTTCAAGCGCGGTGGCAAGCT
GTTCGTCGGCACCAGCAACCCGACCCACTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTCGAGCCGATCCTGG
TCGACGAGGACCAGATCCGGCGCACGCTGGAACAGTGGCACGCCAGCCATGACACCCTCGGCGACGCCCTGGGTGGCGAT
GACGATGGCATGGGCAACCTGGACGTGGCGGCCGGCGACGAGGATCTCGGCGCCACCGGCGGCGATTCCGGCATCGACGC
CAAGGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGCGCCTCGGACATCC
ACTTCGAGCCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTGGCGCGCGCGCCGGTG
AAGCTGAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCACAGCTGGACATCGCCGAGAAGCGCGTCCCGCAGGACGG
CCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGG
TGGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTG
TTCCTGGATGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACTGGCCCGACCGGATCGGGCAAGACCGTGTCGCTGTA
TACCGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCG
TCAACCAGGTACAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGACAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCGGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCGCCCTACAACATCACCA
GCTCGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGCCTGTGCGGCAACTGCAAGCGTCCGTCCAGCCTGCCCGAA
CATGCGCTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCGAGCTGTACGAGGCCGTGGGCTGCGACGA
ATGCACCGAGGGCTACAAGGGCCGGACCGGCGTCTACCAGGTCATGCCGATGACCGATGAGATCGCCACCATCGTGCTGG
CCGGTGGCAACGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGCCAGTCGGCATTGAAGAAG
GCGGCGGCGGGCATCACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.226

99.48

0.549
  pilB Acinetobacter baumannii D1279779

55.536

98.614

0.548
  pilB Legionella pneumophila strain ERS1305867

51.93

98.787

0.513
  pilF Neisseria gonorrhoeae MS11

48.858

98.614

0.482
  pilB Vibrio cholerae strain A1552

48.168

99.307

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

45.804

99.133

0.454
  pilB Vibrio campbellii strain DS40M4

45.343

98.614

0.447
  pilB/pilB1 Synechocystis sp. PCC 6803

35.935

100

0.383
  pilF Thermus thermophilus HB27

36.945

97.574

0.36