Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N5094_RS06065 Genome accession   NZ_CP104755
Coordinates   1396301..1397338 (+) Length   345 a.a.
NCBI ID   WP_011788674.1    Uniprot ID   A0A6N3J320
Organism   Shewanella putrefaciens strain 4H     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1391301..1402338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5094_RS06035 (N5094_06035) rdgB 1392443..1393060 (-) 618 WP_011919679.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  N5094_RS06040 (N5094_06040) - 1393171..1393605 (-) 435 WP_011788669.1 DUF4426 domain-containing protein -
  N5094_RS06045 (N5094_06045) yggU 1393683..1393973 (-) 291 WP_261762640.1 DUF167 family protein YggU -
  N5094_RS06050 (N5094_06050) - 1393973..1394521 (-) 549 WP_011788671.1 YggT family protein -
  N5094_RS06055 (N5094_06055) proC 1394568..1395386 (-) 819 WP_261762641.1 pyrroline-5-carboxylate reductase -
  N5094_RS06060 (N5094_06060) - 1395567..1396265 (-) 699 WP_261762642.1 YggS family pyridoxal phosphate-dependent enzyme -
  N5094_RS06065 (N5094_06065) pilT 1396301..1397338 (+) 1038 WP_011788674.1 type IV pilus twitching motility protein PilT Machinery gene
  N5094_RS06070 (N5094_06070) pilU 1397348..1398460 (+) 1113 WP_011788675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N5094_RS06075 (N5094_06075) - 1398605..1399144 (+) 540 WP_261762643.1 glutathione peroxidase -
  N5094_RS06080 (N5094_06080) hemH 1399183..1400196 (+) 1014 WP_261762644.1 ferrochelatase -
  N5094_RS06085 (N5094_06085) ruvX 1400258..1400683 (-) 426 WP_011919672.1 Holliday junction resolvase RuvX -
  N5094_RS06090 (N5094_06090) - 1400715..1401278 (-) 564 WP_011788679.1 YqgE/AlgH family protein -
  N5094_RS06095 (N5094_06095) yciH 1401391..1401720 (-) 330 WP_115017693.1 stress response translation initiation inhibitor YciH -
  N5094_RS06100 (N5094_06100) - 1401886..1402200 (-) 315 WP_011788681.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38339.04 Da        Isoelectric Point: 7.0500

>NTDB_id=732027 N5094_RS06065 WP_011788674.1 1396301..1397338(+) (pilT) [Shewanella putrefaciens strain 4H]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHRGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASYPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHPNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMSKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=732027 N5094_RS06065 WP_011788674.1 1396301..1397338(+) (pilT) [Shewanella putrefaciens strain 4H]
ATGGAAATCACTGAGTTATTAGCTTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCCAT
GATCCGTGTTGACGGTGAAGTGAGAAAAATTAACCTACCAGCGCTCGATCATCGGGGCGTACACAGCTTAGTGTACGATA
TTATGAATGATAAACAGCGCAAGGACTTTGAAGAGCATTTAGAAATTGATTTTTCGTTTGAAGTGCCCAACTTAGCCCGT
TTTCGTGTAAACGCATTTAACCAATCCCGTGGTGCCGCCGCAGTATTTCGTACCATTCCCAGTGATATATTGTCACTTGA
ACAACTTGGCGCACCGGAGATATTCAAAAAAATTGCCAGTTATCCGCGTGGTTTAGTGTTAGTCACTGGACCGACTGGCT
CGGGTAAGAGTACCACACTTGCAGCAATGGTGGATTACATCAATGAAAACCGCCATGATCATATCTTAACCATTGAAGAC
CCAATTGAATTTGTCCATCCTAATAAACAATGTTTGATTAACCAGAGGGAAGTTCATCGGCATACACACAGTTTTAACGC
TGCCCTCAGGAGTGCTTTGCGTGAGGACCCTGACGTAATTTTAGTGGGGGAGATGCGAGATCTTGAAACCATACGTCTGG
CGATGACCGCTGCAGAAACTGGCCACTTAGTCTTCGGTACTTTACACACAACTTCAGCCGCTAAAACGATTGACCGTGTG
GTGGATGTATTTCCAGCAGGGGAGAAAGACATGGTACGCACTATGTTATCTGAATCATTACAAGCGGTTATTTCACAGAC
GCTTATCAAAAAAATTGGCGGTGGCCGTGTCGCTGCACACGAAATTATGATGGGAACACCTGCAATTCGTAACTTGATCC
GTGAGGACAAAGTCGCACAAATGTACTCTGCCATTCAAACTGGCATGGCTCATGGAATGCAGACTCTGGAGCAATGCCTG
CAAAATTTAGTTAATCGTGGCCTGATCACCCGCGAAGATGCCATGTCGAAAAGCTCAAATAAACAAGCCACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N3J320

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.592

99.42

0.791
  pilT Acinetobacter baumannii strain A118

79.592

99.42

0.791
  pilT Acinetobacter nosocomialis M2

79.592

99.42

0.791
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Acinetobacter baylyi ADP1

80.851

95.362

0.771
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

74.487

98.841

0.736
  pilT Legionella pneumophila strain ERS1305867

74.487

98.841

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.997

97.681

0.713
  pilT Vibrio cholerae strain A1552

72.997

97.681

0.713
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

41.691

99.42

0.414
  pilU Vibrio cholerae strain A1552

41.667

97.391

0.406
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391