Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   N5094_RS06070 Genome accession   NZ_CP104755
Coordinates   1397348..1398460 (+) Length   370 a.a.
NCBI ID   WP_011788675.1    Uniprot ID   A0A6N3J369
Organism   Shewanella putrefaciens strain 4H     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1392348..1403460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5094_RS06035 (N5094_06035) rdgB 1392443..1393060 (-) 618 WP_011919679.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  N5094_RS06040 (N5094_06040) - 1393171..1393605 (-) 435 WP_011788669.1 DUF4426 domain-containing protein -
  N5094_RS06045 (N5094_06045) yggU 1393683..1393973 (-) 291 WP_261762640.1 DUF167 family protein YggU -
  N5094_RS06050 (N5094_06050) - 1393973..1394521 (-) 549 WP_011788671.1 YggT family protein -
  N5094_RS06055 (N5094_06055) proC 1394568..1395386 (-) 819 WP_261762641.1 pyrroline-5-carboxylate reductase -
  N5094_RS06060 (N5094_06060) - 1395567..1396265 (-) 699 WP_261762642.1 YggS family pyridoxal phosphate-dependent enzyme -
  N5094_RS06065 (N5094_06065) pilT 1396301..1397338 (+) 1038 WP_011788674.1 type IV pilus twitching motility protein PilT Machinery gene
  N5094_RS06070 (N5094_06070) pilU 1397348..1398460 (+) 1113 WP_011788675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N5094_RS06075 (N5094_06075) - 1398605..1399144 (+) 540 WP_261762643.1 glutathione peroxidase -
  N5094_RS06080 (N5094_06080) hemH 1399183..1400196 (+) 1014 WP_261762644.1 ferrochelatase -
  N5094_RS06085 (N5094_06085) ruvX 1400258..1400683 (-) 426 WP_011919672.1 Holliday junction resolvase RuvX -
  N5094_RS06090 (N5094_06090) - 1400715..1401278 (-) 564 WP_011788679.1 YqgE/AlgH family protein -
  N5094_RS06095 (N5094_06095) yciH 1401391..1401720 (-) 330 WP_115017693.1 stress response translation initiation inhibitor YciH -
  N5094_RS06100 (N5094_06100) - 1401886..1402200 (-) 315 WP_011788681.1 hypothetical protein -
  N5094_RS06105 (N5094_06105) - 1402379..1402939 (+) 561 WP_011788682.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41097.28 Da        Isoelectric Point: 6.3723

>NTDB_id=732028 N5094_RS06070 WP_011788675.1 1397348..1398460(+) (pilU) [Shewanella putrefaciens strain 4H]
MDVRPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKEPTGSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=732028 N5094_RS06070 WP_011788675.1 1397348..1398460(+) (pilU) [Shewanella putrefaciens strain 4H]
ATGGATGTACGTCCGTTTTTAAAAGTCATCGTTGAGCGTAAAGCCTCTGATTTGTTTATTACCGCGGGCTTTCCTCCCAG
TGCAAAAATTGATGGCGAATTACGTCCCCTCGCCGAGAGTGCATTTACGCCTGCACAGTCTTTAGATTTTGTTGAGTCAC
TCATGACTGAAGCGCAGAAACAGGAATTTCACTCAACCCGTGAATGTAACTTTGCTTTTGCGGCAAAGGATCTCGGTCGT
TTCCGAGTCAGCGCTTTTTGGCAAAGGGAATCACCAGGATGTGTTATGCGCCGGATTGAGACCAAAATTCCCGAAGTCGA
TGACTTAAAATTACCTCCTATTTTAAAAGATCTGGTGATGAGCAAGCGTGGGCTTATCATTATGGTCGGCGGCACAGGTA
CGGGTAAGTCAACCTCATTAGCAGCATTGGTTGGATACCGTAATGCCCATGCCCGTGGCCATATATTGACGATTGAAGAT
CCTGTTGAATTTGTTCACGATCATAGAAAAAGCATTATCACCCAACGTGAAGTAGGCATAGATACGGAGTCATTCGATGC
AGCATTAAAAAGCTCCCTTCGTCAGGCACCCGATGTGATCTTAATCGGTGAAATTCGTACCCAAGAGACGATGGAATTTG
CATTGTCGTTTGCAGAAACAGGTCATCTTTGTATGGCAACCCTACATGCCAATAATGCGAACCAAGCACTCGATCGGATT
ATGCACTTAGTGCCTGAGAGTAAACATCAGCAGTTACTATTTGATTTATCCTTAAATTTACGTGGTATTGTGGCGCAGCA
ATTGATCCCCAAAGTCGATGGCACGGGGCGCCGTGCCGCAATTGAAGTGCTTATTAATACACCTAGGGTTGCGAGTTTAA
TTGCAAAAAATGAACTGCATTTACTCAAGGAAACAATGGCAAAATCGAATGAACAAGGAATGCAGACATTCGATCAAGCT
CTGCTGAAATTATATGTTGATGGCGAAATTAGTTACGCCGATGCCTTACATCATGCTGACTCACCAAACGATTTACGCTT
GATGATTAAATTGCAAAACAAAGAGCCTACCGGTTCAAGCTTTATGGAAGGCGTGACCCTAGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N3J369

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.215

100

0.605
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.87

93.243

0.381
  pilT Vibrio cholerae strain A1552

42.727

89.189

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.727

89.189

0.381
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362