Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GZN30_RS00260 Genome accession   NZ_AP019657
Coordinates   38266..39297 (+) Length   343 a.a.
NCBI ID   WP_075650881.1    Uniprot ID   -
Organism   Vibrio ponticus strain DSM 16217     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 33266..44297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZN30_RS00225 hemW 33551..34726 (-) 1176 WP_075650895.1 radical SAM family heme chaperone HemW -
  GZN30_RS00230 - 34730..35326 (-) 597 WP_075650893.1 XTP/dITP diphosphatase -
  GZN30_RS00235 - 35339..35770 (-) 432 WP_075650891.1 DUF4426 domain-containing protein -
  GZN30_RS00240 yggU 35804..36094 (-) 291 WP_075650889.1 DUF167 family protein YggU -
  GZN30_RS00245 - 36094..36651 (-) 558 WP_075650887.1 YggT family protein -
  GZN30_RS00250 proC 36687..37505 (-) 819 WP_075650885.1 pyrroline-5-carboxylate reductase -
  GZN30_RS00255 - 37531..38241 (-) 711 WP_075650883.1 YggS family pyridoxal phosphate-dependent enzyme -
  GZN30_RS00260 pilT 38266..39297 (+) 1032 WP_075650881.1 type IV pilus twitching motility protein PilT Machinery gene
  GZN30_RS00265 pilU 39313..40413 (+) 1101 WP_408646756.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GZN30_RS00270 lysC 40468..41820 (-) 1353 WP_075650877.1 lysine-sensitive aspartokinase 3 -
  GZN30_RS00275 - 42273..43394 (+) 1122 WP_075650875.1 alanine--glyoxylate aminotransferase family protein -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 37982.41 Da        Isoelectric Point: 6.0891

>NTDB_id=73104 GZN30_RS00260 WP_075650881.1 38266..39297(+) (pilT) [Vibrio ponticus strain DSM 16217]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLSVPAFSHADVHRLVFEIMNDAQRSEFEERLEVDFSFELPDVGR
FRVNAFNQARGCAAVFRTIPTTIPTLEELDAPENFEKIANLEKGLVLVTGPTGSGKSTTLAAMVDYINRNHNKHILTIED
PIEFVHQNQKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKGMVRSMLSESLRAVIAQKLLKRIGGGRVACHEILMATPAIRNLIREDKVAQMYSVIQTGAAQGMQTIEQNA
KQLIARGLVEPQEVEQKIGSLDY

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=73104 GZN30_RS00260 WP_075650881.1 38266..39297(+) (pilT) [Vibrio ponticus strain DSM 16217]
ATGGATATCGCTGAGTTACTGGACTTTAGTGTAAAACATAATGCGTCAGATCTACATCTTTCCGCAGGTGTTTCTCCAAT
GGTGCGCATTGACGGTGAGGTCAGAAAGCTGAGCGTACCTGCTTTTAGCCATGCCGATGTGCACCGTTTAGTCTTTGAGA
TTATGAACGATGCTCAGCGTAGTGAATTTGAGGAGCGTCTCGAAGTCGACTTTTCATTTGAATTACCGGATGTCGGTCGT
TTCCGTGTTAACGCATTTAACCAAGCGCGTGGTTGCGCGGCGGTATTCCGTACGATTCCAACGACCATTCCAACTTTAGA
AGAGTTGGATGCGCCGGAGAACTTTGAAAAAATTGCCAATTTGGAAAAAGGTTTAGTGTTGGTGACCGGTCCGACTGGTT
CGGGTAAATCGACTACATTGGCGGCGATGGTGGATTACATTAATCGCAATCACAATAAGCATATTTTAACGATTGAAGAC
CCAATTGAGTTTGTTCATCAAAATCAAAAATGTCTTATTAACCAACGTGAAGTACATCGTGATACTCACAGCTTTAAAGC
CGCGCTGCGCAGTGCTCTGCGTGAAGACCCAGATGTGATTTTGGTGGGTGAGTTGCGCGATCAAGAGACGATTAGCTTGG
CACTGACTGCGGCTGAAACGGGGCACTTGGTGTTTGGTACGCTGCATACCAGCTCTGCGGCAAAGACTATCGACCGTATT
ATCGATGTGTTCCCTGGCAGCGATAAAGGTATGGTGCGCTCAATGCTTTCAGAATCATTGCGTGCGGTAATCGCCCAGAA
GCTGCTCAAGCGCATTGGTGGTGGTCGTGTTGCTTGCCATGAAATCTTAATGGCGACACCAGCGATTCGTAACTTGATCC
GTGAAGATAAAGTGGCACAGATGTACTCAGTGATCCAAACTGGTGCCGCGCAAGGCATGCAGACGATTGAGCAAAATGCC
AAACAGTTGATTGCTCGCGGCTTGGTTGAACCGCAAGAAGTGGAGCAGAAGATTGGTTCACTTGATTACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

89.645

98.542

0.883
  pilT Vibrio cholerae strain A1552

89.645

98.542

0.883
  pilT Acinetobacter baumannii D1279779

71.687

96.793

0.694
  pilT Acinetobacter baumannii strain A118

71.687

96.793

0.694
  pilT Acinetobacter nosocomialis M2

71.386

96.793

0.691
  pilT Pseudomonas stutzeri DSM 10701

68.843

98.251

0.676
  pilT Acinetobacter baylyi ADP1

70.213

95.918

0.673
  pilT Pseudomonas aeruginosa PAK

68.249

98.251

0.671
  pilT Legionella pneumophila strain Lp02

67.781

95.918

0.65
  pilT Legionella pneumophila strain ERS1305867

67.781

95.918

0.65
  pilT Neisseria meningitidis 8013

66.066

97.085

0.641
  pilT Neisseria gonorrhoeae MS11

64.793

98.542

0.638
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.727

96.21

0.507
  pilU Vibrio cholerae strain A1552

43.114

97.376

0.42
  pilU Pseudomonas stutzeri DSM 10701

42.683

95.627

0.408
  pilU Acinetobacter baylyi ADP1

40.625

93.294

0.379

Multiple sequence alignment