Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GZN30_RS00265 Genome accession   NZ_AP019657
Coordinates   39313..40413 (+) Length   366 a.a.
NCBI ID   WP_408646756.1    Uniprot ID   -
Organism   Vibrio ponticus strain DSM 16217     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 34313..45413
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZN30_RS00230 - 34730..35326 (-) 597 WP_075650893.1 XTP/dITP diphosphatase -
  GZN30_RS00235 - 35339..35770 (-) 432 WP_075650891.1 DUF4426 domain-containing protein -
  GZN30_RS00240 yggU 35804..36094 (-) 291 WP_075650889.1 DUF167 family protein YggU -
  GZN30_RS00245 - 36094..36651 (-) 558 WP_075650887.1 YggT family protein -
  GZN30_RS00250 proC 36687..37505 (-) 819 WP_075650885.1 pyrroline-5-carboxylate reductase -
  GZN30_RS00255 - 37531..38241 (-) 711 WP_075650883.1 YggS family pyridoxal phosphate-dependent enzyme -
  GZN30_RS00260 pilT 38266..39297 (+) 1032 WP_075650881.1 type IV pilus twitching motility protein PilT Machinery gene
  GZN30_RS00265 pilU 39313..40413 (+) 1101 WP_408646756.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GZN30_RS00270 lysC 40468..41820 (-) 1353 WP_075650877.1 lysine-sensitive aspartokinase 3 -
  GZN30_RS00275 - 42273..43394 (+) 1122 WP_075650875.1 alanine--glyoxylate aminotransferase family protein -
  GZN30_RS00280 - 43478..45241 (-) 1764 WP_075650873.1 PglL family O-oligosaccharyltransferase -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40848.57 Da        Isoelectric Point: 5.9410

>NTDB_id=73105 GZN30_RS00265 WP_408646756.1 39313..40413(+) (pilU) [Vibrio ponticus strain DSM 16217]
MDSFLQGMKQHKASDLYITVDAPVLYRIDGELQPSGEKLSAEQVFELLDSIMDESRQDEFRASREANFAVVREYGRFRVS
AFFQRELPGAVIRRIETEIPTFAELHLPEVLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNSHRSGHILTVEDPIEF
VHQHQKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMAFAETGHLCMATLHANNANQALERILHLV
PKEKKEQFLFDLSMNLKGVVGQQLLRDKSGQGRHGAFEILLNTPRVADLIRRGELHELKATMAKSQQSGMQTFDQALYQL
VVDGKISEEDALHSADSANDLRIMLKTLSGDGFASGSLADVKIDMN

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=73105 GZN30_RS00265 WP_408646756.1 39313..40413(+) (pilU) [Vibrio ponticus strain DSM 16217]
ATGGATAGTTTTCTTCAAGGAATGAAGCAGCATAAAGCCTCGGATCTTTATATTACAGTTGATGCGCCGGTACTCTATCG
CATTGATGGTGAGTTGCAGCCAAGTGGTGAGAAGTTATCTGCCGAGCAAGTGTTTGAACTGCTTGATTCCATCATGGATG
AATCACGTCAAGACGAGTTTCGTGCCAGCCGAGAAGCGAATTTCGCTGTCGTACGCGAGTATGGACGCTTTCGTGTATCC
GCTTTTTTTCAACGCGAGTTACCCGGCGCAGTGATAAGACGGATTGAAACGGAGATCCCGACTTTTGCCGAGTTACACTT
GCCGGAAGTACTTAAAGATTTATCCATTGCTAAGCGCGGTTTAGTGTTGGTTGTTGGAGCGACAGGTTCAGGTAAATCGA
CCACGATGGCGGCGATGACAGGCTATCGCAATAGCCATCGCTCTGGGCATATCCTCACCGTAGAAGACCCGATTGAATTT
GTTCACCAACATCAAAAATGTATCGTCACTCAGCGTGAAGTAGGCTTGGATACCGAGAGTTATGAAGTAGCGCTGAAAAA
CTCGCTGCGCCAAGCGCCGGATATGATCTTGATTGGTGAGATCCGCAGTCGTGAAACGATGGAATATGCGATGGCGTTTG
CGGAGACAGGGCATCTTTGTATGGCGACGTTGCATGCCAATAATGCTAACCAAGCGCTTGAACGAATTTTGCATTTGGTG
CCGAAAGAGAAGAAAGAGCAGTTTTTGTTTGATCTATCAATGAACCTAAAAGGTGTCGTAGGGCAACAGTTGCTGCGTGA
TAAGAGTGGACAAGGTCGCCATGGCGCATTTGAAATATTACTCAATACCCCGCGAGTGGCTGATTTAATTCGCCGCGGCG
AACTGCATGAGTTAAAAGCGACCATGGCAAAATCTCAGCAATCCGGCATGCAAACTTTTGATCAAGCGCTGTATCAGCTG
GTGGTTGATGGCAAAATCAGTGAAGAAGATGCGCTACATAGTGCAGACTCTGCCAATGACTTACGCATTATGCTTAAGAC
TTTGAGTGGGGACGGTTTTGCTTCTGGCTCTTTAGCGGATGTCAAAATTGATATGAATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.644

99.727

0.814
  pilU Pseudomonas stutzeri DSM 10701

58.79

94.809

0.557
  pilU Acinetobacter baylyi ADP1

55.301

95.355

0.527
  pilT Legionella pneumophila strain Lp02

43.293

89.617

0.388
  pilT Legionella pneumophila strain ERS1305867

43.293

89.617

0.388
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.484

84.699

0.385
  pilT Pseudomonas aeruginosa PAK

42.462

88.798

0.377
  pilT Acinetobacter baylyi ADP1

41.515

90.164

0.374
  pilT Acinetobacter baumannii strain A118

41.159

89.617

0.369
  pilT Acinetobacter baumannii D1279779

41.159

89.617

0.369
  pilT Acinetobacter nosocomialis M2

41.159

89.617

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.923

88.798

0.363
  pilT Vibrio cholerae strain A1552

41.176

88.251

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.176

88.251

0.363

Multiple sequence alignment