Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GZK95_RS01850 Genome accession   NZ_AP019654
Coordinates   373530..374561 (+) Length   343 a.a.
NCBI ID   WP_075709760.1    Uniprot ID   A0A1Q9HC46
Organism   Vibrio panuliri strain JCM 19500     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 368530..379561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZK95_RS01815 hemW 368809..369984 (-) 1176 WP_075716404.1 radical SAM family heme chaperone HemW -
  GZK95_RS01820 - 369988..370584 (-) 597 WP_075716403.1 XTP/dITP diphosphatase -
  GZK95_RS01825 - 370597..371028 (-) 432 WP_075709755.1 DUF4426 domain-containing protein -
  GZK95_RS01830 yggU 371062..371352 (-) 291 WP_075709756.1 DUF167 family protein YggU -
  GZK95_RS01835 - 371352..371909 (-) 558 WP_075709757.1 YggT family protein -
  GZK95_RS01840 proC 371951..372769 (-) 819 WP_075716402.1 pyrroline-5-carboxylate reductase -
  GZK95_RS01845 - 372792..373505 (-) 714 WP_075716401.1 YggS family pyridoxal phosphate-dependent enzyme -
  GZK95_RS01850 pilT 373530..374561 (+) 1032 WP_075709760.1 type IV pilus twitching motility protein PilT Machinery gene
  GZK95_RS01855 pilU 374571..375677 (+) 1107 WP_075716400.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GZK95_RS01860 lysC 375765..377117 (-) 1353 WP_075716399.1 lysine-sensitive aspartokinase 3 -
  GZK95_RS01865 - 377619..378740 (+) 1122 WP_075716398.1 alanine--glyoxylate aminotransferase family protein -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 38101.59 Da        Isoelectric Point: 6.0891

>NTDB_id=73076 GZK95_RS01850 WP_075709760.1 373530..374561(+) (pilT) [Vibrio panuliri strain JCM 19500]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLSVPEFSHADVHRLVFEIMNDAQRSEFEERLEVDFSFELPDVGR
FRVNAFNQARGCAAVFRTIPTTIPTLEQLEAPQIFEKIANIEKGLVLVTGPTGSGKSTTLAAMVDYINRNHNKHILTIED
PIEFVHQNQKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRIGGGRVACHEIMMATPAIRNLIREDKVAQMYSVIQTGASQGMQTIEQNA
KQLIAKGLVEPQEVEQKIGSLDY

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=73076 GZK95_RS01850 WP_075709760.1 373530..374561(+) (pilT) [Vibrio panuliri strain JCM 19500]
ATGGATATCGCTGAGTTACTGGACTTTAGTGTAAAACATAATGCGTCAGATCTACATCTTTCTGCAGGTGTTTCTCCAAT
GGTGCGTATTGACGGTGAGGTCAGAAAGCTGAGCGTACCTGAGTTTAGCCATGCTGACGTCCACCGTTTGGTGTTTGAGA
TTATGAACGATGCGCAGCGCAGTGAATTTGAGGAGCGTCTCGAAGTCGACTTTTCATTTGAATTACCTGATGTCGGTCGT
TTTCGCGTCAATGCGTTTAATCAGGCGCGGGGGTGTGCAGCGGTATTTCGTACCATTCCTACGACGATTCCTACCTTAGA
GCAACTTGAAGCACCGCAGATATTTGAAAAAATCGCCAATATTGAAAAAGGTTTAGTACTGGTGACGGGACCGACAGGTT
CCGGTAAGTCAACCACACTTGCGGCGATGGTGGATTACATCAATCGTAATCACAACAAACACATTTTAACTATTGAAGAC
CCGATTGAGTTTGTGCACCAAAATCAGAAGTGTCTCGTCAACCAGCGAGAAGTACACCGTGATACGCACAGTTTCAAAGC
GGCGTTACGCAGTGCACTACGTGAAGACCCTGATGTCATTTTGGTGGGTGAGTTACGTGACCAAGAGACGATTAGTTTGG
CGTTGACGGCGGCAGAGACCGGTCATTTAGTGTTTGGGACACTGCATACCAGTTCAGCGGCAAAAACGATTGACCGTATT
ATTGATGTATTTCCAGGAAGTGATAAAGACATGGTGCGCTCAATGTTGTCTGAATCGCTGCGTGCGGTTATTGCACAAAA
ACTTCTCAAGCGCATCGGTGGTGGGCGTGTCGCATGCCATGAAATCATGATGGCGACCCCAGCGATTCGTAACCTGATCC
GTGAAGATAAAGTGGCTCAAATGTACTCAGTTATTCAGACGGGCGCTTCGCAGGGGATGCAAACTATCGAGCAAAATGCT
AAGCAATTGATCGCAAAAGGATTGGTAGAACCACAAGAGGTTGAGCAAAAAATCGGATCGTTGGATTACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q9HC46

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

89.053

98.542

0.878
  pilT Vibrio cholerae strain A1552

89.053

98.542

0.878
  pilT Acinetobacter baumannii D1279779

71.988

96.793

0.697
  pilT Acinetobacter baumannii strain A118

71.988

96.793

0.697
  pilT Acinetobacter nosocomialis M2

71.687

96.793

0.694
  pilT Pseudomonas stutzeri DSM 10701

69.436

98.251

0.682
  pilT Acinetobacter baylyi ADP1

70.517

95.918

0.676
  pilT Pseudomonas aeruginosa PAK

68.843

98.251

0.676
  pilT Legionella pneumophila strain Lp02

68.693

95.918

0.659
  pilT Legionella pneumophila strain ERS1305867

68.693

95.918

0.659
  pilT Neisseria meningitidis 8013

65.465

97.085

0.636
  pilT Neisseria gonorrhoeae MS11

64.201

98.542

0.633
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.818

96.21

0.499
  pilU Vibrio cholerae strain A1552

42.216

97.376

0.411
  pilU Pseudomonas stutzeri DSM 10701

42.683

95.627

0.408
  pilU Acinetobacter baylyi ADP1

40.312

93.294

0.376

Multiple sequence alignment