Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GZK95_RS01855 Genome accession   NZ_AP019654
Coordinates   374571..375677 (+) Length   368 a.a.
NCBI ID   WP_075716400.1    Uniprot ID   -
Organism   Vibrio panuliri strain JCM 19500     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 369571..380677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZK95_RS01820 - 369988..370584 (-) 597 WP_075716403.1 XTP/dITP diphosphatase -
  GZK95_RS01825 - 370597..371028 (-) 432 WP_075709755.1 DUF4426 domain-containing protein -
  GZK95_RS01830 yggU 371062..371352 (-) 291 WP_075709756.1 DUF167 family protein YggU -
  GZK95_RS01835 - 371352..371909 (-) 558 WP_075709757.1 YggT family protein -
  GZK95_RS01840 proC 371951..372769 (-) 819 WP_075716402.1 pyrroline-5-carboxylate reductase -
  GZK95_RS01845 - 372792..373505 (-) 714 WP_075716401.1 YggS family pyridoxal phosphate-dependent enzyme -
  GZK95_RS01850 pilT 373530..374561 (+) 1032 WP_075709760.1 type IV pilus twitching motility protein PilT Machinery gene
  GZK95_RS01855 pilU 374571..375677 (+) 1107 WP_075716400.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GZK95_RS01860 lysC 375765..377117 (-) 1353 WP_075716399.1 lysine-sensitive aspartokinase 3 -
  GZK95_RS01865 - 377619..378740 (+) 1122 WP_075716398.1 alanine--glyoxylate aminotransferase family protein -
  GZK95_RS01870 - 378797..380593 (-) 1797 WP_075713987.1 PglL family O-oligosaccharyltransferase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41231.09 Da        Isoelectric Point: 5.7971

>NTDB_id=73077 GZK95_RS01855 WP_075716400.1 374571..375677(+) (pilU) [Vibrio panuliri strain JCM 19500]
MAMDTFLQGMKQQKASDLYITVGAPILYRIDGDLQPVGEPLTEQQVFALLDSVMEQVRQDEFRASREANFAVVREFGRFR
VSAFFQRELPGAVIRRIETEIPTFESLHLPEVLKDLATAKRGLVLVVGATGSGKSTTMAAMTGYRNSHRSGHILTVEDPI
EFVHQHQKCIVTQREVGLDTESYEIALKNSLRQAPDMILIGEIRSRETMEYAMAFAETGHLCMATLHANNANQALERILH
LVPKEKKEQFLFDLSMNLKGVVGQQLLRDKDGQGRHGAFEILLNTPRIADLIRRGDLHELKSTMAKSQQTGMQTFDQALY
QLVIDDKVGEDDALHSADSANDLRIMLKTQRGDGFSSGLLNDVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=73077 GZK95_RS01855 WP_075716400.1 374571..375677(+) (pilU) [Vibrio panuliri strain JCM 19500]
ATGGCAATGGATACCTTTCTTCAAGGAATGAAACAGCAAAAGGCCTCGGATCTATACATTACCGTGGGGGCTCCGATTCT
GTACCGTATTGATGGTGACTTGCAGCCCGTGGGAGAGCCCCTGACTGAGCAGCAAGTTTTTGCTTTGTTGGATTCGGTGA
TGGAGCAAGTGCGTCAAGATGAATTTCGTGCGTCTCGTGAAGCGAATTTTGCTGTTGTGCGTGAGTTTGGCCGTTTTCGT
GTATCGGCTTTTTTCCAACGCGAACTACCAGGTGCGGTGATTAGACGTATTGAAACGGAAATTCCTACCTTTGAGTCTTT
GCATTTACCGGAGGTTTTAAAAGATCTCGCCACGGCAAAACGAGGGTTAGTGCTAGTGGTAGGTGCGACGGGCTCGGGAA
AATCAACCACGATGGCGGCAATGACGGGGTATCGGAATTCGCATCGTTCTGGGCATATTTTGACGGTGGAAGATCCCATC
GAGTTTGTTCATCAACATCAAAAGTGCATTGTGACTCAGCGTGAAGTAGGGTTAGACACAGAAAGTTATGAGATAGCGCT
TAAGAATTCATTACGGCAAGCGCCTGATATGATTCTGATTGGTGAGATTCGTAGCCGTGAAACGATGGAATATGCGATGG
CGTTTGCGGAGACCGGGCACTTATGTATGGCAACCCTGCATGCCAATAATGCCAACCAAGCTCTAGAGCGGATTCTTCAT
TTGGTTCCGAAGGAAAAGAAAGAGCAATTTTTATTCGATCTCTCGATGAACTTAAAAGGGGTTGTTGGTCAGCAGTTGCT
GCGCGATAAAGATGGTCAAGGTCGCCATGGTGCATTTGAGATATTACTCAATACTCCTAGAATCGCTGATCTGATCCGTC
GTGGAGATTTGCATGAACTTAAGTCTACGATGGCGAAGTCGCAGCAAACGGGTATGCAGACATTTGATCAAGCGCTGTAT
CAGTTGGTTATTGATGACAAGGTGGGCGAAGATGATGCTTTGCATAGCGCTGATTCCGCCAATGATCTGCGAATTATGCT
TAAAACGCAACGTGGGGATGGTTTCTCTTCGGGGCTATTAAATGATGTAAAGATCGATATGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

79.891

100

0.799
  pilU Pseudomonas stutzeri DSM 10701

57.714

95.109

0.549
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain Lp02

44.512

89.13

0.397
  pilT Legionella pneumophila strain ERS1305867

44.512

89.13

0.397
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.037

88.859

0.391
  pilT Pseudomonas aeruginosa PAK

42.378

89.13

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.462

88.315

0.375
  pilT Vibrio cholerae strain A1552

42.462

88.315

0.375
  pilT Pseudomonas stutzeri DSM 10701

42.073

89.13

0.375
  pilT Acinetobacter baylyi ADP1

42.675

85.326

0.364
  pilT Acinetobacter nosocomialis M2

42.357

85.326

0.361
  pilT Acinetobacter baumannii D1279779

42.357

85.326

0.361
  pilT Acinetobacter baumannii strain A118

42.357

85.326

0.361

Multiple sequence alignment