Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GZI12_RS12245 Genome accession   NZ_AP019651
Coordinates   2621425..2622456 (-) Length   343 a.a.
NCBI ID   WP_162047224.1    Uniprot ID   -
Organism   Vibrio taketomensis strain C4III291     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2616425..2627456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZI12_RS12230 - 2617153..2618274 (-) 1122 WP_162047222.1 alanine--glyoxylate aminotransferase family protein -
  GZI12_RS12235 lysC 2618895..2620247 (+) 1353 WP_162047223.1 lysine-sensitive aspartokinase 3 -
  GZI12_RS12240 pilU 2620308..2621411 (-) 1104 WP_408647130.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GZI12_RS12245 pilT 2621425..2622456 (-) 1032 WP_162047224.1 type IV pilus twitching motility protein PilT Machinery gene
  GZI12_RS12250 - 2622481..2623191 (+) 711 WP_162047225.1 YggS family pyridoxal phosphate-dependent enzyme -
  GZI12_RS12255 proC 2623243..2624061 (+) 819 WP_162047226.1 pyrroline-5-carboxylate reductase -
  GZI12_RS12260 - 2624105..2624659 (+) 555 WP_162047227.1 YggT family protein -
  GZI12_RS12265 yggU 2624659..2624949 (+) 291 WP_162047228.1 DUF167 family protein YggU -
  GZI12_RS12270 - 2624976..2625407 (+) 432 WP_162047229.1 DUF4426 domain-containing protein -
  GZI12_RS12275 - 2625420..2626016 (+) 597 WP_162047230.1 XTP/dITP diphosphatase -
  GZI12_RS12280 hemW 2626020..2627195 (+) 1176 WP_162047231.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 38176.70 Da        Isoelectric Point: 6.0896

>NTDB_id=73056 GZI12_RS12245 WP_162047224.1 2621425..2622456(-) (pilT) [Vibrio taketomensis strain C4III291]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLSVPAFTHAEVHRLVFEIMNDAQRSEFEEHLEVDFSFELPEVGR
FRVNAFNQSRGCAAVFRTIPTSIPTLQDLDAPEIFEKIANFEKGLVLVTGPTGSGKSTTLAAMVDFINRNQNKHILTIED
PIEFVHQNQKCLINQREVHRDTHSFKKALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRIGGGRVACHEIMMATPAIRNLIREDKVAQMYSVIQTGAAQGMQTIEQNA
RQLIVRGLVEPEEVEQKIGSLTY

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=73056 GZI12_RS12245 WP_162047224.1 2621425..2622456(-) (pilT) [Vibrio taketomensis strain C4III291]
ATGGATATCGCTGAGTTACTGGACTTTAGTGTAAAACATAATGCGTCAGATCTACATCTTTCTGCAGGTGTTTCTCCAAT
GGTGCGCATTGACGGTGAAGTACGCAAGTTGAGTGTTCCTGCTTTTACTCATGCAGAAGTACATCGTTTAGTGTTTGAAA
TCATGAATGATGCGCAGCGCAGTGAGTTTGAAGAACATCTTGAAGTCGACTTTTCATTTGAGTTACCTGAAGTTGGTCGC
TTCCGTGTTAACGCTTTCAACCAATCTCGAGGATGTGCGGCGGTATTCCGTACCATTCCAACCAGTATTCCGACGTTGCA
AGATCTCGATGCACCGGAAATTTTTGAGAAAATCGCGAACTTTGAAAAAGGCTTAGTGCTGGTTACAGGGCCGACTGGTT
CAGGTAAATCGACGACGCTTGCCGCGATGGTTGATTTTATTAACCGTAATCAGAATAAGCACATTTTGACCATCGAAGAC
CCGATTGAATTTGTCCATCAGAACCAAAAATGTTTGATCAACCAACGTGAAGTGCATCGTGATACACATAGCTTTAAAAA
GGCATTGCGCAGCGCACTTCGTGAAGACCCTGATGTGATTTTGGTGGGGGAGTTGCGTGACCAAGAAACGATCAGTTTGG
CATTAACAGCGGCAGAAACGGGTCACTTGGTATTTGGTACTTTGCATACCAGTTCAGCCGCAAAAACGATTGACCGTATT
ATCGACGTATTTCCTGGTAGTGATAAAGATATGGTTCGCTCGATGCTTTCAGAATCCTTACGTGCGGTTATTGCGCAGAA
GCTACTGAAACGCATTGGTGGTGGACGTGTTGCGTGTCATGAGATCATGATGGCAACGCCAGCTATTCGTAACTTGATAC
GTGAGGATAAAGTCGCGCAAATGTATTCAGTGATTCAAACGGGTGCAGCGCAAGGTATGCAAACTATTGAGCAAAATGCG
AGACAACTGATTGTTCGAGGCTTGGTTGAACCGGAAGAAGTTGAACAAAAAATTGGCTCGCTCACTTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

88.462

98.542

0.872
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.462

98.542

0.872
  pilT Acinetobacter baumannii strain A118

71.084

96.793

0.688
  pilT Acinetobacter nosocomialis M2

71.084

96.793

0.688
  pilT Acinetobacter baumannii D1279779

71.084

96.793

0.688
  pilT Acinetobacter baylyi ADP1

69.909

95.918

0.671
  pilT Pseudomonas stutzeri DSM 10701

68.249

98.251

0.671
  pilT Pseudomonas aeruginosa PAK

67.359

98.251

0.662
  pilT Legionella pneumophila strain Lp02

68.085

95.918

0.653
  pilT Legionella pneumophila strain ERS1305867

68.085

95.918

0.653
  pilT Neisseria meningitidis 8013

65.465

97.085

0.636
  pilT Neisseria gonorrhoeae MS11

64.201

98.542

0.633
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.909

96.21

0.49
  pilU Pseudomonas stutzeri DSM 10701

41.617

97.376

0.405
  pilU Vibrio cholerae strain A1552

42.025

95.044

0.399
  pilU Acinetobacter baylyi ADP1

41.25

93.294

0.385

Multiple sequence alignment