Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GZI12_RS12240 Genome accession   NZ_AP019651
Coordinates   2620308..2621411 (-) Length   367 a.a.
NCBI ID   WP_408647130.1    Uniprot ID   -
Organism   Vibrio taketomensis strain C4III291     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2615308..2626411
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZI12_RS12225 - 2615352..2617121 (+) 1770 WP_162047221.1 PglL family O-oligosaccharyltransferase -
  GZI12_RS12230 - 2617153..2618274 (-) 1122 WP_162047222.1 alanine--glyoxylate aminotransferase family protein -
  GZI12_RS12235 lysC 2618895..2620247 (+) 1353 WP_162047223.1 lysine-sensitive aspartokinase 3 -
  GZI12_RS12240 pilU 2620308..2621411 (-) 1104 WP_408647130.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GZI12_RS12245 pilT 2621425..2622456 (-) 1032 WP_162047224.1 type IV pilus twitching motility protein PilT Machinery gene
  GZI12_RS12250 - 2622481..2623191 (+) 711 WP_162047225.1 YggS family pyridoxal phosphate-dependent enzyme -
  GZI12_RS12255 proC 2623243..2624061 (+) 819 WP_162047226.1 pyrroline-5-carboxylate reductase -
  GZI12_RS12260 - 2624105..2624659 (+) 555 WP_162047227.1 YggT family protein -
  GZI12_RS12265 yggU 2624659..2624949 (+) 291 WP_162047228.1 DUF167 family protein YggU -
  GZI12_RS12270 - 2624976..2625407 (+) 432 WP_162047229.1 DUF4426 domain-containing protein -
  GZI12_RS12275 - 2625420..2626016 (+) 597 WP_162047230.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 41309.20 Da        Isoelectric Point: 6.6922

>NTDB_id=73055 GZI12_RS12240 WP_408647130.1 2620308..2621411(-) (pilU) [Vibrio taketomensis strain C4III291]
MIDAFLQEMNAQKASDLYITVGAPVLYRVDGELRAHGEVLSEQNVMDLLNAIMDEPRRADYNATREANFAVVRNFGRFRV
SAFFQRELPGAVIRRIETQIPTFESLRLPDVLQNLCIAKRGLVLVVGATGSGKSTTMAAMTGYRNRHRAGHIITVEDPIE
FVHEHHKCIVTQREVGLDTESYDVALKNSLRQAPDMILIGEIRTRETMEFAMAFAETGHLCMATLHANNANQALERILHL
VPKEKKEQFLFDLSNNLRGVIGQQLLRDKYGSGRHGVFEILLNSPRVSDLIRRGELHELKSTMAKSQQIGMQTFDQALYQ
LIVDDKISVEDAMHSADSANDLRIMLKTQHGDSFNTKTQSSISIDMN

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=73055 GZI12_RS12240 WP_408647130.1 2620308..2621411(-) (pilU) [Vibrio taketomensis strain C4III291]
ATGATTGATGCATTTCTGCAAGAGATGAACGCCCAAAAAGCGTCCGATTTATATATCACAGTGGGCGCACCAGTGCTGTA
TCGCGTTGATGGTGAATTGCGTGCGCACGGCGAGGTCTTAAGCGAACAGAATGTTATGGATTTGCTCAATGCCATCATGG
ACGAACCACGCCGAGCGGATTATAACGCCACGCGTGAAGCCAATTTTGCGGTAGTGAGAAATTTTGGACGTTTTCGTGTT
TCAGCATTTTTTCAACGCGAACTTCCCGGTGCGGTCATTCGTCGTATCGAAACGCAAATTCCAACGTTTGAATCTTTGCG
TTTACCGGATGTATTACAAAATTTATGCATTGCTAAGCGCGGCCTAGTTTTGGTGGTAGGGGCGACAGGCTCTGGTAAAT
CGACCACAATGGCTGCGATGACAGGGTATCGAAATCGCCACCGAGCAGGACATATTATAACGGTTGAAGATCCGATCGAA
TTTGTTCATGAGCACCACAAGTGTATCGTGACTCAACGTGAAGTCGGATTGGACACGGAAAGTTATGATGTCGCGCTCAA
GAACTCATTGCGCCAAGCGCCAGATATGATTTTGATTGGTGAGATACGGACGCGTGAAACCATGGAGTTTGCGATGGCGT
TTGCTGAAACCGGTCATTTATGTATGGCAACCCTGCACGCAAACAATGCTAACCAAGCTCTAGAGCGGATATTGCATTTG
GTGCCGAAAGAGAAGAAAGAGCAGTTTTTGTTTGATCTCTCAAATAACCTACGGGGAGTTATCGGTCAGCAATTGCTTAG
GGATAAATATGGTAGTGGTCGTCATGGGGTGTTTGAGATCTTGCTGAATAGCCCAAGGGTTTCGGACTTGATTCGTCGTG
GAGAGTTACACGAATTGAAATCGACTATGGCTAAATCGCAGCAGATTGGTATGCAAACCTTTGACCAAGCGCTATACCAG
CTTATTGTTGACGATAAAATCAGTGTCGAAGATGCGATGCACAGTGCGGACTCCGCTAACGATTTACGCATTATGCTTAA
AACTCAACATGGTGATAGCTTTAATACTAAAACACAATCTTCTATTAGCATTGATATGAATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

79.452

99.455

0.79
  pilU Pseudomonas stutzeri DSM 10701

56.772

94.55

0.537
  pilU Acinetobacter baylyi ADP1

51.734

94.278

0.488
  pilT Acinetobacter baylyi ADP1

43.902

89.373

0.392
  pilT Legionella pneumophila strain ERS1305867

42.945

88.828

0.381
  pilT Legionella pneumophila strain Lp02

42.945

88.828

0.381
  pilT Acinetobacter baumannii strain A118

42.769

88.556

0.379
  pilT Acinetobacter nosocomialis M2

42.769

88.556

0.379
  pilT Acinetobacter baumannii D1279779

42.769

88.556

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.202

89.101

0.376
  pilT Pseudomonas aeruginosa PAK

41.896

89.101

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.73

84.741

0.371
  pilT Vibrio cholerae strain A1552

43.73

84.741

0.371
  pilT Pseudomonas stutzeri DSM 10701

41.59

89.101

0.371

Multiple sequence alignment