Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Vt282_RS01815 Genome accession   NZ_AP019649
Coordinates   355973..357004 (+) Length   343 a.a.
NCBI ID   WP_162047224.1    Uniprot ID   -
Organism   Vibrio taketomensis strain C4III282     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 350973..362004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Vt282_RS01780 hemW 351234..352409 (-) 1176 WP_162062391.1 radical SAM family heme chaperone HemW -
  Vt282_RS01785 - 352413..353009 (-) 597 WP_162062392.1 XTP/dITP diphosphatase -
  Vt282_RS01790 - 353022..353453 (-) 432 WP_162062393.1 DUF4426 domain-containing protein -
  Vt282_RS01795 yggU 353480..353770 (-) 291 WP_162047228.1 DUF167 family protein YggU -
  Vt282_RS01800 - 353770..354324 (-) 555 WP_162062394.1 YggT family protein -
  Vt282_RS01805 proC 354368..355186 (-) 819 WP_162062395.1 pyrroline-5-carboxylate reductase -
  Vt282_RS01810 - 355238..355948 (-) 711 WP_162062396.1 YggS family pyridoxal phosphate-dependent enzyme -
  Vt282_RS01815 pilT 355973..357004 (+) 1032 WP_162047224.1 type IV pilus twitching motility protein PilT Machinery gene
  Vt282_RS01820 pilU 357018..358121 (+) 1104 WP_408647130.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Vt282_RS01825 lysC 358182..359534 (-) 1353 WP_162062397.1 lysine-sensitive aspartokinase 3 -
  Vt282_RS01830 - 360155..361276 (+) 1122 WP_162062398.1 pyridoxal-phosphate-dependent aminotransferase family protein -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 38176.70 Da        Isoelectric Point: 6.0896

>NTDB_id=72999 Vt282_RS01815 WP_162047224.1 355973..357004(+) (pilT) [Vibrio taketomensis strain C4III282]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLSVPAFTHAEVHRLVFEIMNDAQRSEFEEHLEVDFSFELPEVGR
FRVNAFNQSRGCAAVFRTIPTSIPTLQDLDAPEIFEKIANFEKGLVLVTGPTGSGKSTTLAAMVDFINRNQNKHILTIED
PIEFVHQNQKCLINQREVHRDTHSFKKALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRIGGGRVACHEIMMATPAIRNLIREDKVAQMYSVIQTGAAQGMQTIEQNA
RQLIVRGLVEPEEVEQKIGSLTY

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=72999 Vt282_RS01815 WP_162047224.1 355973..357004(+) (pilT) [Vibrio taketomensis strain C4III282]
ATGGATATCGCTGAGTTACTGGACTTTAGTGTAAAACATAATGCGTCAGATCTACATCTTTCTGCAGGTGTTTCTCCAAT
GGTGCGCATTGACGGTGAAGTACGCAAGTTGAGTGTTCCTGCTTTTACTCATGCAGAAGTACATCGTTTAGTGTTTGAAA
TCATGAATGATGCGCAGCGCAGTGAGTTTGAAGAACATCTTGAAGTCGACTTTTCATTTGAATTACCTGAAGTTGGTCGC
TTCCGTGTTAACGCTTTCAACCAATCTCGAGGATGTGCGGCGGTATTCCGTACCATTCCAACCAGTATTCCGACGTTGCA
AGATCTCGATGCGCCGGAAATTTTTGAGAAAATCGCGAACTTTGAAAAAGGCTTAGTGCTGGTTACAGGGCCGACTGGCT
CAGGTAAATCGACGACGCTTGCCGCGATGGTTGATTTTATTAACCGTAATCAGAATAAGCATATTTTGACCATCGAAGAC
CCGATTGAATTTGTCCATCAGAACCAAAAATGTTTGATCAACCAACGTGAAGTGCATCGTGATACACATAGCTTTAAAAA
GGCATTGCGCAGCGCACTTCGTGAAGACCCTGATGTGATTTTGGTGGGGGAGTTGCGTGACCAAGAAACAATCAGTTTGG
CATTAACAGCGGCAGAAACGGGTCACTTGGTATTTGGTACTTTGCATACCAGTTCAGCCGCAAAAACGATTGACCGTATT
ATCGACGTATTTCCAGGTAGTGATAAAGATATGGTTCGCTCGATGCTTTCAGAATCCTTACGCGCGGTTATTGCGCAGAA
GCTACTGAAACGCATTGGTGGTGGACGTGTTGCATGTCATGAGATCATGATGGCAACGCCAGCTATTCGTAACTTGATAC
GTGAAGATAAAGTCGCGCAAATGTATTCAGTGATTCAAACGGGTGCAGCGCAAGGCATGCAAACCATTGAGCAAAATGCG
AGACAACTGATTGTTCGAGGCTTGGTTGAGCCGGAAGAAGTTGAACAAAAAATTGGCTCGCTCACTTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

88.462

98.542

0.872
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.462

98.542

0.872
  pilT Acinetobacter baumannii strain A118

71.084

96.793

0.688
  pilT Acinetobacter nosocomialis M2

71.084

96.793

0.688
  pilT Acinetobacter baumannii D1279779

71.084

96.793

0.688
  pilT Acinetobacter baylyi ADP1

69.909

95.918

0.671
  pilT Pseudomonas stutzeri DSM 10701

68.249

98.251

0.671
  pilT Pseudomonas aeruginosa PAK

67.359

98.251

0.662
  pilT Legionella pneumophila strain Lp02

68.085

95.918

0.653
  pilT Legionella pneumophila strain ERS1305867

68.085

95.918

0.653
  pilT Neisseria meningitidis 8013

65.465

97.085

0.636
  pilT Neisseria gonorrhoeae MS11

64.201

98.542

0.633
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.909

96.21

0.49
  pilU Pseudomonas stutzeri DSM 10701

41.617

97.376

0.405
  pilU Vibrio cholerae strain A1552

42.025

95.044

0.399
  pilU Acinetobacter baylyi ADP1

41.25

93.294

0.385

Multiple sequence alignment