Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Vt282_RS01820 Genome accession   NZ_AP019649
Coordinates   357018..358121 (+) Length   367 a.a.
NCBI ID   WP_408647130.1    Uniprot ID   -
Organism   Vibrio taketomensis strain C4III282     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 352018..363121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Vt282_RS01785 - 352413..353009 (-) 597 WP_162062392.1 XTP/dITP diphosphatase -
  Vt282_RS01790 - 353022..353453 (-) 432 WP_162062393.1 DUF4426 domain-containing protein -
  Vt282_RS01795 yggU 353480..353770 (-) 291 WP_162047228.1 DUF167 family protein YggU -
  Vt282_RS01800 - 353770..354324 (-) 555 WP_162062394.1 YggT family protein -
  Vt282_RS01805 proC 354368..355186 (-) 819 WP_162062395.1 pyrroline-5-carboxylate reductase -
  Vt282_RS01810 - 355238..355948 (-) 711 WP_162062396.1 YggS family pyridoxal phosphate-dependent enzyme -
  Vt282_RS01815 pilT 355973..357004 (+) 1032 WP_162047224.1 type IV pilus twitching motility protein PilT Machinery gene
  Vt282_RS01820 pilU 357018..358121 (+) 1104 WP_408647130.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Vt282_RS01825 lysC 358182..359534 (-) 1353 WP_162062397.1 lysine-sensitive aspartokinase 3 -
  Vt282_RS01830 - 360155..361276 (+) 1122 WP_162062398.1 pyridoxal-phosphate-dependent aminotransferase family protein -
  Vt282_RS01835 - 361308..363076 (-) 1769 Protein_353 PglL family O-oligosaccharyltransferase -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 41309.20 Da        Isoelectric Point: 6.6922

>NTDB_id=73000 Vt282_RS01820 WP_408647130.1 357018..358121(+) (pilU) [Vibrio taketomensis strain C4III282]
MIDAFLQEMNAQKASDLYITVGAPVLYRVDGELRAHGEVLSEQNVMDLLNAIMDEPRRADYNATREANFAVVRNFGRFRV
SAFFQRELPGAVIRRIETQIPTFESLRLPDVLQNLCIAKRGLVLVVGATGSGKSTTMAAMTGYRNRHRAGHIITVEDPIE
FVHEHHKCIVTQREVGLDTESYDVALKNSLRQAPDMILIGEIRTRETMEFAMAFAETGHLCMATLHANNANQALERILHL
VPKEKKEQFLFDLSNNLRGVIGQQLLRDKYGSGRHGVFEILLNSPRVSDLIRRGELHELKSTMAKSQQIGMQTFDQALYQ
LIVDDKISVEDAMHSADSANDLRIMLKTQHGDSFNTKTQSSISIDMN

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=73000 Vt282_RS01820 WP_408647130.1 357018..358121(+) (pilU) [Vibrio taketomensis strain C4III282]
ATGATTGATGCATTTCTGCAGGAGATGAATGCCCAAAAAGCGTCCGATTTGTATATCACCGTGGGTGCACCAGTGTTGTA
TCGCGTTGATGGTGAATTGCGTGCGCACGGCGAGGTCTTAAGCGAACAGAATGTTATGGATTTACTCAATGCCATCATGG
ACGAACCACGCCGAGCAGATTATAACGCCACGCGTGAAGCCAATTTTGCGGTAGTGAGGAATTTTGGACGTTTTCGTGTC
TCAGCATTTTTTCAACGCGAGCTTCCCGGTGCAGTCATCCGGCGTATCGAAACACAAATTCCAACGTTTGAATCTTTGCG
TTTACCGGATGTATTGCAAAATTTATGTATTGCTAAGCGCGGCCTAGTTTTGGTGGTTGGAGCGACAGGCTCTGGTAAAT
CGACCACAATGGCTGCGATGACGGGGTATCGAAATCGCCACCGAGCGGGACATATTATAACGGTTGAAGATCCGATCGAA
TTTGTTCATGAGCACCACAAGTGTATCGTGACTCAACGTGAAGTCGGATTGGACACGGAAAGCTACGATGTCGCGCTCAA
AAACTCATTGCGCCAAGCGCCAGATATGATTTTGATTGGTGAGATACGGACGCGTGAAACCATGGAGTTTGCGATGGCGT
TTGCTGAAACCGGTCATTTATGTATGGCAACCCTGCACGCAAACAATGCTAACCAAGCTCTAGAGCGGATATTGCATTTG
GTGCCGAAAGAGAAGAAAGAGCAGTTTTTGTTTGATCTCTCAAATAACCTACGGGGAGTTATCGGTCAGCAATTGCTTAG
GGATAAATATGGTAGTGGTCGACATGGTGTGTTTGAGATCTTGCTGAATAGCCCAAGGGTTTCGGACTTGATTCGTCGTG
GAGAGTTACACGAATTGAAATCGACCATGGCTAAATCACAGCAGATTGGTATGCAAACCTTTGACCAAGCGCTGTACCAG
CTTATTGTTGACGATAAAATCAGTGTCGAAGATGCGATGCACAGTGCGGACTCCGCTAACGATTTACGCATTATGCTTAA
AACTCAACATGGCGATAGCTTTAATACTAAAACACAATCTTCTATTAGCATTGATATGAATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

79.452

99.455

0.79
  pilU Pseudomonas stutzeri DSM 10701

56.772

94.55

0.537
  pilU Acinetobacter baylyi ADP1

51.734

94.278

0.488
  pilT Acinetobacter baylyi ADP1

43.902

89.373

0.392
  pilT Legionella pneumophila strain ERS1305867

42.945

88.828

0.381
  pilT Legionella pneumophila strain Lp02

42.945

88.828

0.381
  pilT Acinetobacter baumannii strain A118

42.769

88.556

0.379
  pilT Acinetobacter nosocomialis M2

42.769

88.556

0.379
  pilT Acinetobacter baumannii D1279779

42.769

88.556

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.202

89.101

0.376
  pilT Pseudomonas aeruginosa PAK

41.896

89.101

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.73

84.741

0.371
  pilT Vibrio cholerae strain A1552

43.73

84.741

0.371
  pilT Pseudomonas stutzeri DSM 10701

41.59

89.101

0.371

Multiple sequence alignment