Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N4T19_RS14415 Genome accession   NZ_CP104377
Coordinates   3116095..3117138 (+) Length   347 a.a.
NCBI ID   WP_021027363.1    Uniprot ID   A0AAW4Y2E3
Organism   Comamonas sp. PR12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3111095..3122138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4T19_RS14390 (N4T19_14390) norR 3111234..3112853 (+) 1620 WP_260718370.1 nitric oxide reductase transcriptional regulator NorR -
  N4T19_RS14395 (N4T19_14395) - 3112881..3113483 (+) 603 WP_165841247.1 zinc-ribbon domain containing protein -
  N4T19_RS14400 (N4T19_14400) - 3113542..3113958 (-) 417 WP_116927672.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  N4T19_RS14405 (N4T19_14405) - 3114041..3115177 (-) 1137 WP_116927661.1 pyridoxal-phosphate-dependent aminotransferase family protein -
  N4T19_RS14410 (N4T19_14410) - 3115283..3116011 (-) 729 WP_182343914.1 YggS family pyridoxal phosphate-dependent enzyme -
  N4T19_RS14415 (N4T19_14415) pilT 3116095..3117138 (+) 1044 WP_021027363.1 type IV pilus twitching motility protein PilT Machinery gene
  N4T19_RS14420 (N4T19_14420) - 3117249..3117857 (+) 609 WP_116927662.1 cyclic nucleotide-binding domain-containing protein -
  N4T19_RS14425 (N4T19_14425) pilU 3117931..3119067 (+) 1137 WP_116927663.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N4T19_RS14430 (N4T19_14430) - 3119144..3120070 (+) 927 WP_021027360.1 NAD(P)-dependent oxidoreductase -
  N4T19_RS14435 (N4T19_14435) - 3120277..3120936 (-) 660 Protein_2808 BON domain-containing protein -
  N4T19_RS14440 (N4T19_14440) - 3120936..3121535 (-) 600 WP_116927665.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38356.03 Da        Isoelectric Point: 6.7744

>NTDB_id=729223 N4T19_RS14415 WP_021027363.1 3116095..3117138(+) (pilT) [Comamonas sp. PR12]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLDHKQVHAMVYDIMSDAQRKQYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGSGAVFRTIPSKILTLEQLNAPKIFADLSLKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPQTLSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTSNSMGMQTLDQN
LTDLVRRNVISPAEARAKAKTPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=729223 N4T19_RS14415 WP_021027363.1 3116095..3117138(+) (pilT) [Comamonas sp. PR12]
GTGGATATCACCCAATTGCTGGCATTCAGTGTCAAAAATAAAGCGTCGGACTTGCACCTGTCTGCAGGCCTGCCGCCGAT
GATCCGGGTCCATGGCGATGTGCGCCGCATCAATGTCGATCCGCTGGACCACAAACAGGTCCATGCGATGGTCTACGACA
TCATGAGCGATGCCCAGCGCAAGCAGTATGAAGAGTTTCTCGAGGTCGACTTCTCGTTCGAGATCGAAGGCCTGGCCCGT
TTCCGTGTCAATGCCTTCAACCAGAACCGTGGCTCGGGCGCCGTGTTCCGGACCATTCCGAGCAAGATCCTGACCCTCGA
GCAGCTCAACGCCCCCAAGATCTTTGCCGACCTGTCGCTCAAGCCGCGCGGCCTGGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCCACCACCTTGGCGGCCATGGTCAACTACCTCAACGAAAACGAATACGGCCACATCCTGACGGTGGAAGAC
CCGATCGAATTCGTGCACGAATCCAAGAAATGCCTGATCAACCAGCGCGAAGTGGGCCCGCAGACGCTGTCGTTCAGCGC
AGCGCTGCGCTCGGCGCTGCGTGAAGACCCGGACGCGATCCTGGTGGGCGAAATGCGTGACCTCGAAACCATCCGCCTGG
CGATGACGGCCGCAGAAACCGGCCACTTGGTGTTCGGCACCCTGCACACCTCCAGCGCCGCCAAGACCATTGACCGGATC
ATCGACGTCTTCCCCGCCGAAGAAAAGGAAATGGTCCGCGCGATGCTGTCCGAATCGCTGCAGGCTGTGATCTCCCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCCGCCCACGAGATCATGCTGGGCACGCCCGCCATCCGCAACCTGA
TCCGTGAGGCCAAGGTCGCGCAGATGTACTCCACCATCCAGACAAGCAACAGCATGGGCATGCAGACGCTGGACCAGAAC
CTGACCGACCTGGTGCGCCGCAATGTGATCAGCCCGGCCGAGGCCCGCGCCAAGGCGAAGACGCCAGAAAACTTCCCAGG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.971

99.424

0.775
  pilT Acinetobacter nosocomialis M2

77.391

99.424

0.769
  pilT Acinetobacter baumannii D1279779

77.391

99.424

0.769
  pilT Acinetobacter baumannii strain A118

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

76.812

99.424

0.764
  pilT Acinetobacter baylyi ADP1

75.652

99.424

0.752
  pilT Legionella pneumophila strain Lp02

73.156

97.695

0.715
  pilT Legionella pneumophila strain ERS1305867

73.156

97.695

0.715
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.343

97.406

0.666
  pilT Vibrio cholerae strain A1552

68.343

97.406

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

42.899

99.424

0.427
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Pseudomonas stutzeri DSM 10701

41.159

99.424

0.409