Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   N4T19_RS14425 Genome accession   NZ_CP104377
Coordinates   3117931..3119067 (+) Length   378 a.a.
NCBI ID   WP_116927663.1    Uniprot ID   A0A363RD62
Organism   Comamonas sp. PR12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3112931..3124067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4T19_RS14400 (N4T19_14400) - 3113542..3113958 (-) 417 WP_116927672.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  N4T19_RS14405 (N4T19_14405) - 3114041..3115177 (-) 1137 WP_116927661.1 pyridoxal-phosphate-dependent aminotransferase family protein -
  N4T19_RS14410 (N4T19_14410) - 3115283..3116011 (-) 729 WP_182343914.1 YggS family pyridoxal phosphate-dependent enzyme -
  N4T19_RS14415 (N4T19_14415) pilT 3116095..3117138 (+) 1044 WP_021027363.1 type IV pilus twitching motility protein PilT Machinery gene
  N4T19_RS14420 (N4T19_14420) - 3117249..3117857 (+) 609 WP_116927662.1 cyclic nucleotide-binding domain-containing protein -
  N4T19_RS14425 (N4T19_14425) pilU 3117931..3119067 (+) 1137 WP_116927663.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N4T19_RS14430 (N4T19_14430) - 3119144..3120070 (+) 927 WP_021027360.1 NAD(P)-dependent oxidoreductase -
  N4T19_RS14435 (N4T19_14435) - 3120277..3120936 (-) 660 Protein_2808 BON domain-containing protein -
  N4T19_RS14440 (N4T19_14440) - 3120936..3121535 (-) 600 WP_116927665.1 SIS domain-containing protein -
  N4T19_RS14445 (N4T19_14445) - 3121724..3122170 (-) 447 WP_116927666.1 YraN family protein -
  N4T19_RS14450 (N4T19_14450) rsmI 3122161..3123093 (+) 933 WP_260718378.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41985.36 Da        Isoelectric Point: 6.7686

>NTDB_id=729224 N4T19_RS14425 WP_116927663.1 3117931..3119067(+) (pilU) [Comamonas sp. PR12]
MERDQASKFINDLLKLMVQRNGSDLFITADFPPAIKIDGKVTKVSSQPLSPTHSLTLARSIMSDKQVAEFEKTKECNFAI
SPSGIGRFRVNAFMQQGHVGLVLRTIPLTLPTIDGLGVPQVLKDLAMTKRGLCIMVGATGSGKSTTLAAMVDWRNENSYG
HIITVEDPVEFVHPHKNCIVTQREVGLDTDNWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRGLVSQRLLSKQDSKGRVAAVEVMLNSPLISDMIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLYEANMITYEDALRNADSVNDLRLQIKLGSQRAKRSDLAEGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=729224 N4T19_RS14425 WP_116927663.1 3117931..3119067(+) (pilU) [Comamonas sp. PR12]
ATGGAACGGGATCAGGCCAGTAAGTTCATCAACGACCTGTTGAAGCTGATGGTGCAGCGCAATGGCAGCGATTTGTTCAT
CACCGCTGACTTTCCGCCGGCCATCAAGATCGATGGCAAGGTCACCAAGGTCTCCAGCCAGCCGCTGTCGCCCACCCACT
CGCTGACGCTGGCCCGCTCGATCATGAGCGACAAGCAGGTCGCCGAGTTCGAAAAGACCAAGGAATGCAATTTCGCGATC
TCGCCCTCGGGCATCGGGCGCTTCCGGGTCAATGCCTTCATGCAGCAAGGCCATGTGGGTCTGGTACTGCGGACCATTCC
ATTGACCCTGCCAACGATTGACGGCCTGGGCGTGCCCCAGGTGCTCAAGGACCTGGCGATGACCAAGCGCGGCCTGTGCA
TCATGGTGGGCGCCACCGGCTCGGGCAAGTCCACCACCTTGGCGGCGATGGTCGACTGGCGCAACGAAAACTCCTACGGC
CACATCATCACCGTCGAAGACCCGGTGGAATTTGTCCACCCGCACAAGAACTGCATCGTGACCCAGCGCGAAGTGGGCCT
GGACACCGACAACTGGGAAGCGGCGCTGAAAAACACCTTGCGCCAGGCGCCCGACGTCATCCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCACGCGGTGGCCTTTGCCGAAACCGGTCACCTGTGCCTGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCGCGCAGCTGCTGATGGACCTGTCGCTCAACCT
GCGCGGCCTGGTGTCGCAGCGCCTTTTGAGCAAGCAGGACAGCAAGGGCCGCGTCGCCGCCGTCGAGGTAATGCTCAACT
CGCCGCTGATCTCCGACATGATCTTCAAGGGCGAAGTGGGCGAGATCAAGGAGATCATGAAGAAAAGCCGCAACCTGGGC
ATGCAGACCTTTGACCAGGCGCTGTTTGACCTCTACGAGGCCAACATGATCACCTACGAAGACGCGCTGCGCAACGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTGGGCAGCCAGCGCGCCAAGCGCAGTGACCTGGCCGAGGGCACGGAGC
ACCTGACAATCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A363RD62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.568

96.825

0.606
  pilU Acinetobacter baylyi ADP1

57.382

94.974

0.545
  pilU Vibrio cholerae strain A1552

53.911

94.709

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

89.153

0.397
  pilT Pseudomonas aeruginosa PAK

43.027

89.153

0.384
  pilT Acinetobacter baumannii D1279779

43.243

88.095

0.381
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baumannii strain A118

43.243

88.095

0.381
  pilT Legionella pneumophila strain ERS1305867

42.943

88.095

0.378
  pilT Legionella pneumophila strain Lp02

42.943

88.095

0.378
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.543

89.153

0.37