Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N4J17_RS13085 Genome accession   NZ_CP104311
Coordinates   2671964..2672998 (-) Length   344 a.a.
NCBI ID   WP_198321626.1    Uniprot ID   -
Organism   Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2666964..2677998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4J17_RS13060 (N4J17_13075) - 2667541..2668323 (+) 783 WP_198321622.1 succinate dehydrogenase iron-sulfur subunit -
  N4J17_RS13065 (N4J17_13080) - 2668320..2668577 (+) 258 WP_198321623.1 succinate dehydrogenase assembly factor 2 -
  N4J17_RS13070 (N4J17_13085) - 2668571..2668993 (+) 423 WP_198321624.1 protein YgfX -
  N4J17_RS13075 (N4J17_13090) - 2668974..2670794 (+) 1821 WP_232470184.1 VWA domain-containing protein -
  N4J17_RS13080 (N4J17_13095) pilU 2670830..2671948 (-) 1119 WP_198321625.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N4J17_RS13085 (N4J17_13100) pilT 2671964..2672998 (-) 1035 WP_198321626.1 type IV pilus twitching motility protein PilT Machinery gene
  N4J17_RS13090 (N4J17_13105) - 2673053..2673748 (+) 696 WP_198321627.1 YggS family pyridoxal phosphate-dependent enzyme -
  N4J17_RS13095 (N4J17_13110) proC 2673813..2674637 (+) 825 WP_198321628.1 pyrroline-5-carboxylate reductase -
  N4J17_RS13100 (N4J17_13115) - 2674641..2675219 (+) 579 WP_198321629.1 YggT family protein -
  N4J17_RS13105 (N4J17_13120) - 2675655..2676599 (-) 945 WP_198324346.1 IS110 family transposase -
  N4J17_RS13110 (N4J17_13125) - 2677078..2677554 (+) 477 WP_232470250.1 PAS domain S-box protein -
  N4J17_RS13115 (N4J17_13130) - 2677637..2677786 (+) 150 WP_232470251.1 hypothetical protein -
  N4J17_RS13120 (N4J17_13135) - 2677823..2677882 (+) 60 Protein_2572 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38349.04 Da        Isoelectric Point: 6.7752

>NTDB_id=728578 N4J17_RS13085 WP_198321626.1 2671964..2672998(-) (pilT) [Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)]
MDIAELLAFSVKNKSSDLHLSSGLPPMIRVDGDIRRINVPPLEHKEVHALIYDIMNDKQRRDFEEFLETDFSFELPGVAR
FRVNAFNQDRGAAAVFRTIPSKVLTLEELGCPKFFQDVTRQPRGLIVVTGPTGSGKSTTLAAMIDYINSNDYSHILTIED
PIEFVHQSKKCLINQREVHRNTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMIRSMLSESLQAVISQALVKKVGGGRTAAWEIMVGTPAIRNLIREAKVAQMYSTIQTGRKDGMQTLDQHL
QELVERGIITRQVAREVAVNKAAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=728578 N4J17_RS13085 WP_198321626.1 2671964..2672998(-) (pilT) [Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)]
ATGGATATCGCCGAACTGCTCGCCTTCTCAGTCAAGAACAAATCTTCCGACCTGCACCTTTCTTCCGGGCTGCCGCCGAT
GATCCGCGTCGACGGCGACATCCGGCGCATCAACGTGCCGCCGCTGGAGCACAAGGAAGTACACGCGCTCATATATGACA
TCATGAACGACAAACAGCGGCGCGACTTCGAGGAGTTCCTGGAAACCGATTTCTCATTCGAACTGCCGGGTGTCGCCCGT
TTCCGGGTCAATGCCTTCAACCAGGACCGCGGCGCGGCGGCTGTGTTCCGGACCATCCCTTCCAAAGTCCTCACGCTGGA
GGAACTGGGCTGCCCGAAGTTCTTCCAGGACGTCACCCGCCAGCCACGCGGCCTGATCGTGGTCACCGGACCGACGGGTT
CGGGCAAGTCCACCACGCTGGCGGCCATGATCGACTACATCAACTCCAACGACTATTCCCACATTCTGACCATCGAAGAT
CCGATCGAGTTCGTCCACCAGAGCAAGAAATGCCTGATCAACCAACGCGAGGTGCACCGCAACACCTTGGGCTTCAACGA
AGCCCTGCGCTCGGCCCTGCGCGAAGACCCGGACGTGATCCTCGTGGGCGAAATGCGCGACCTGGAAACCATCCGCCTTG
CGCTGACCGCGGCCGAAACCGGACACCTGGTGTTCGGCACTTTGCATACCAGTTCCGCCGCCAAGACCATCGACCGCATC
GTCGACGTGTTCCCGGCCGCCGAGAAAGACATGATCCGCTCCATGCTGTCGGAATCGCTGCAGGCGGTCATTTCGCAGGC
CCTCGTCAAAAAAGTCGGCGGCGGCCGGACGGCGGCCTGGGAGATCATGGTCGGCACTCCGGCCATCCGCAACCTGATCC
GCGAGGCCAAGGTGGCCCAGATGTATTCGACGATCCAGACGGGCCGTAAGGACGGCATGCAGACCCTGGATCAGCACCTG
CAGGAACTGGTGGAGAGAGGGATCATCACCCGTCAGGTCGCCCGCGAAGTCGCCGTCAACAAAGCCGCGTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.065

98.256

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.07

100

0.791
  pilT Acinetobacter nosocomialis M2

78.488

100

0.785
  pilT Acinetobacter baumannii D1279779

78.488

100

0.785
  pilT Acinetobacter baumannii strain A118

78.488

100

0.785
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

74.709

100

0.747
  pilT Legionella pneumophila strain ERS1305867

74.709

100

0.747
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.246

100

0.674
  pilT Vibrio cholerae strain A1552

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

40

97.384

0.39
  pilU Acinetobacter baylyi ADP1

39.458

96.512

0.381