Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   N4J17_RS13080 Genome accession   NZ_CP104311
Coordinates   2670830..2671948 (-) Length   372 a.a.
NCBI ID   WP_198321625.1    Uniprot ID   -
Organism   Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2665830..2676948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4J17_RS13055 (N4J17_13070) - 2666100..2667509 (+) 1410 WP_198321621.1 class II fumarate hydratase -
  N4J17_RS13060 (N4J17_13075) - 2667541..2668323 (+) 783 WP_198321622.1 succinate dehydrogenase iron-sulfur subunit -
  N4J17_RS13065 (N4J17_13080) - 2668320..2668577 (+) 258 WP_198321623.1 succinate dehydrogenase assembly factor 2 -
  N4J17_RS13070 (N4J17_13085) - 2668571..2668993 (+) 423 WP_198321624.1 protein YgfX -
  N4J17_RS13075 (N4J17_13090) - 2668974..2670794 (+) 1821 WP_232470184.1 VWA domain-containing protein -
  N4J17_RS13080 (N4J17_13095) pilU 2670830..2671948 (-) 1119 WP_198321625.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N4J17_RS13085 (N4J17_13100) pilT 2671964..2672998 (-) 1035 WP_198321626.1 type IV pilus twitching motility protein PilT Machinery gene
  N4J17_RS13090 (N4J17_13105) - 2673053..2673748 (+) 696 WP_198321627.1 YggS family pyridoxal phosphate-dependent enzyme -
  N4J17_RS13095 (N4J17_13110) proC 2673813..2674637 (+) 825 WP_198321628.1 pyrroline-5-carboxylate reductase -
  N4J17_RS13100 (N4J17_13115) - 2674641..2675219 (+) 579 WP_198321629.1 YggT family protein -
  N4J17_RS13105 (N4J17_13120) - 2675655..2676599 (-) 945 WP_198324346.1 IS110 family transposase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41540.92 Da        Isoelectric Point: 6.4183

>NTDB_id=728577 N4J17_RS13080 WP_198321625.1 2670830..2671948(-) (pilU) [Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)]
MEFESLLKLMVLKKASDLFITAAKEPCMKLNGNLVPLASTKLTSEQARQLVLGIMNQRQRDEFENTNECNFALSAAGLGR
FRVSAFVQRNSPGMVLRRIETEIPTVEQLNLPPILNDLVMAKRGLILFVGATGTGKSTSLAAMLKYRNEHSSGHIITIED
PLEFVHPHAGCIVTQREVGIDTESYEVALKNTLRQAPDVILIGEIRTRDTMQQAITFAETGHLCLSTLHANNANQALDRI
LHFFPEDMHPQLLMDLSLNLRAIIAQQLVKRADGKGRYPAVEILINTPLVSDLIRQGEIHKLKEVMKQSREQGMQTFDQA
LFELFKAGRIGYEDALYSADSKNEVRLMIKLSEEGSIDKYAPTDASIQIVDN

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=728577 N4J17_RS13080 WP_198321625.1 2670830..2671948(-) (pilU) [Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)]
ATGGAATTCGAGTCACTGCTCAAGCTGATGGTGCTGAAAAAAGCATCCGATCTCTTCATCACTGCGGCCAAGGAACCCTG
CATGAAGCTCAACGGCAACCTCGTACCGCTGGCCAGCACCAAACTCACCAGTGAACAGGCGCGCCAGCTCGTCCTCGGCA
TCATGAACCAGCGCCAGCGCGACGAATTCGAGAACACCAACGAGTGCAATTTCGCCCTCTCAGCGGCCGGACTGGGCCGG
TTCCGGGTCAGCGCCTTCGTCCAGCGGAACAGCCCCGGCATGGTGCTACGCCGGATCGAGACCGAAATTCCCACAGTGGA
GCAGCTCAACCTGCCGCCCATCCTCAACGATCTGGTGATGGCCAAGCGCGGTCTGATCCTGTTCGTGGGCGCGACCGGCA
CCGGCAAGTCCACTTCGCTGGCGGCGATGCTCAAGTACCGCAACGAGCATTCCAGCGGCCACATCATCACCATCGAGGAT
CCCCTGGAGTTCGTCCACCCGCACGCGGGCTGCATCGTCACCCAGCGCGAGGTCGGCATCGACACCGAATCCTACGAAGT
CGCACTGAAGAACACCCTGCGGCAGGCGCCAGACGTGATCCTGATCGGCGAGATCCGCACCCGTGACACCATGCAGCAGG
CCATCACCTTCGCCGAGACCGGTCATCTCTGTCTGAGTACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGCATC
CTCCATTTCTTCCCGGAAGACATGCATCCGCAGCTGCTCATGGATCTGTCGCTGAACCTGCGCGCGATCATCGCCCAGCA
GCTCGTGAAACGCGCCGACGGCAAGGGGCGCTATCCGGCGGTGGAAATCCTGATCAACACGCCCCTGGTCTCCGACCTGA
TCCGCCAGGGAGAGATCCACAAGCTCAAGGAGGTCATGAAGCAATCGCGCGAACAGGGCATGCAGACCTTTGATCAGGCG
CTGTTCGAACTGTTCAAGGCGGGCCGGATCGGCTACGAAGATGCCCTCTACTCGGCCGATTCCAAGAACGAAGTCCGCTT
GATGATCAAGCTCAGCGAAGAAGGCAGCATCGACAAATACGCGCCCACGGACGCATCGATCCAGATCGTCGACAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.079

95.161

0.648
  pilU Acinetobacter baylyi ADP1

64.426

95.968

0.618
  pilU Vibrio cholerae strain A1552

57.714

94.086

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.103

93.548

0.403
  pilT Legionella pneumophila strain Lp02

40.816

92.204

0.376
  pilT Legionella pneumophila strain ERS1305867

40.816

92.204

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

89.785

0.368
  pilT Vibrio cholerae strain A1552

41.018

89.785

0.368
  pilT Acinetobacter baylyi ADP1

39.296

91.667

0.36