Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   W01_RS08455 Genome accession   NZ_AP019547
Coordinates   1893639..1894682 (-) Length   347 a.a.
NCBI ID   WP_173053794.1    Uniprot ID   -
Organism   Candidatus Nitrotoga sp. AM1P     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1888639..1899682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  W01_RS08430 (W01_16480) - 1889342..1889995 (+) 654 WP_173055865.1 response regulator -
  W01_RS08435 (W01_16490) - 1890066..1890623 (-) 558 WP_173053786.1 class I SAM-dependent methyltransferase -
  W01_RS08440 (W01_16500) rpiA 1890869..1891528 (+) 660 WP_173053788.1 ribose-5-phosphate isomerase RpiA -
  W01_RS08445 (W01_16510) phoU 1891596..1892303 (+) 708 WP_173053790.1 phosphate signaling complex protein PhoU -
  W01_RS08450 (W01_16520) pilU 1892478..1893614 (-) 1137 WP_173053792.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  W01_RS08455 (W01_16530) pilT 1893639..1894682 (-) 1044 WP_173053794.1 type IV pilus twitching motility protein PilT Machinery gene
  W01_RS08460 (W01_16540) - 1894843..1895541 (+) 699 WP_173053796.1 YggS family pyridoxal phosphate-dependent enzyme -
  W01_RS08465 (W01_16550) proC 1895538..1896353 (+) 816 WP_173053797.1 pyrroline-5-carboxylate reductase -
  W01_RS08470 (W01_16560) - 1896347..1896910 (+) 564 WP_173053799.1 YggT family protein -
  W01_RS08475 (W01_16570) - 1896913..1897215 (+) 303 WP_320416777.1 DUF167 family protein -
  W01_RS08480 (W01_16580) - 1897415..1897825 (-) 411 WP_173053803.1 hypothetical protein -
  W01_RS08485 (W01_16590) - 1898676..1898957 (-) 282 WP_173053805.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38230.25 Da        Isoelectric Point: 7.3504

>NTDB_id=72848 W01_RS08455 WP_173053794.1 1893639..1894682(-) (pilT) [Candidatus Nitrotoga sp. AM1P]
MDITELLAFGVKNKASDLHLSAGLPPMIRVHGDIRKINLPVMLHTEVHAMVYDIMNDGQRKIYEENKEIDFSFEIPGLAR
FRVNAFVHQRGAGAVMRTIPSKILTLADLKAPKIFETISDYPRGMVLVTGPTGSGKSTTLAAMVNHRNEHEMGHILTVED
PIEFVHESKKCLVNQREVGAHTLSFQNALRSALREDPDIILVGEMRDLETIRLAMSAAETGHLVFGTLHTSSAAKTIDRI
IDVFPADEKEMVRAMLSESLRAVISQTLLKTKDGAGRVAAHEIMICTPAIRNLIREAKVPQMYSSIQTGGALGMQTLDQC
LQDLVKRNVVSSAEARTKAANKDTFVG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=72848 W01_RS08455 WP_173053794.1 1893639..1894682(-) (pilT) [Candidatus Nitrotoga sp. AM1P]
ATGGACATTACCGAACTGCTTGCTTTTGGTGTCAAAAACAAGGCTTCTGATTTGCACCTGTCTGCTGGATTACCGCCGAT
GATTCGTGTGCATGGCGACATACGCAAAATCAACCTGCCGGTGATGTTACATACCGAAGTGCATGCCATGGTATATGACA
TCATGAATGACGGGCAGCGCAAAATTTATGAAGAAAATAAAGAAATCGATTTTTCTTTTGAGATTCCGGGGCTCGCACGT
TTTCGCGTAAATGCCTTTGTTCATCAGCGCGGTGCGGGTGCGGTGATGCGTACCATTCCATCTAAAATATTGACCTTGGC
AGACCTCAAAGCTCCAAAAATTTTTGAGACTATTTCCGATTACCCGCGTGGCATGGTGTTAGTGACAGGCCCAACCGGAT
CGGGTAAGTCCACCACGTTGGCAGCGATGGTAAATCATCGCAACGAACATGAAATGGGGCACATTTTGACTGTGGAGGAT
CCGATTGAATTTGTGCATGAAAGTAAAAAGTGTTTGGTCAACCAGCGCGAAGTTGGCGCGCATACACTTTCATTTCAAAA
CGCTTTACGTAGCGCATTGCGCGAAGATCCAGACATTATCCTGGTAGGTGAAATGCGCGACTTGGAAACTATACGTCTGG
CCATGTCCGCGGCTGAAACAGGTCATTTGGTATTTGGAACACTGCATACCAGTTCTGCGGCCAAGACCATCGACCGCATC
ATTGACGTTTTCCCCGCGGACGAAAAAGAAATGGTGCGCGCCATGTTGTCAGAATCATTGCGAGCAGTTATTTCCCAAAC
CTTACTTAAAACCAAAGATGGTGCCGGGCGTGTGGCAGCCCATGAAATTATGATTTGTACTCCAGCCATCCGCAATCTGA
TTCGGGAAGCCAAGGTGCCACAGATGTATTCTTCCATCCAGACCGGCGGTGCTCTCGGAATGCAGACGCTGGATCAATGC
CTGCAAGATCTGGTCAAACGCAACGTTGTATCATCTGCAGAAGCGCGCACCAAGGCAGCTAACAAGGATACGTTTGTCGG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

73.988

99.712

0.738
  pilT Acinetobacter baumannii D1279779

73.988

99.712

0.738
  pilT Acinetobacter baumannii strain A118

73.988

99.712

0.738
  pilT Pseudomonas stutzeri DSM 10701

73.913

99.424

0.735
  pilT Pseudomonas aeruginosa PAK

73.333

99.424

0.729
  pilT Acinetobacter baylyi ADP1

73.333

99.424

0.729
  pilT Legionella pneumophila strain Lp02

71.304

99.424

0.709
  pilT Legionella pneumophila strain ERS1305867

71.304

99.424

0.709
  pilT Neisseria meningitidis 8013

69.565

99.424

0.692
  pilT Neisseria gonorrhoeae MS11

69.275

99.424

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.456

97.406

0.657
  pilT Vibrio cholerae strain A1552

67.456

97.406

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

99.424

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.687

96.542

0.412
  pilU Acinetobacter baylyi ADP1

41.964

96.83

0.406
  pilU Vibrio cholerae strain A1552

41.194

96.542

0.398

Multiple sequence alignment