Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   W01_RS08450 Genome accession   NZ_AP019547
Coordinates   1892478..1893614 (-) Length   378 a.a.
NCBI ID   WP_173053792.1    Uniprot ID   -
Organism   Candidatus Nitrotoga sp. AM1P     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1887478..1898614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  W01_RS08430 (W01_16480) - 1889342..1889995 (+) 654 WP_173055865.1 response regulator -
  W01_RS08435 (W01_16490) - 1890066..1890623 (-) 558 WP_173053786.1 class I SAM-dependent methyltransferase -
  W01_RS08440 (W01_16500) rpiA 1890869..1891528 (+) 660 WP_173053788.1 ribose-5-phosphate isomerase RpiA -
  W01_RS08445 (W01_16510) phoU 1891596..1892303 (+) 708 WP_173053790.1 phosphate signaling complex protein PhoU -
  W01_RS08450 (W01_16520) pilU 1892478..1893614 (-) 1137 WP_173053792.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  W01_RS08455 (W01_16530) pilT 1893639..1894682 (-) 1044 WP_173053794.1 type IV pilus twitching motility protein PilT Machinery gene
  W01_RS08460 (W01_16540) - 1894843..1895541 (+) 699 WP_173053796.1 YggS family pyridoxal phosphate-dependent enzyme -
  W01_RS08465 (W01_16550) proC 1895538..1896353 (+) 816 WP_173053797.1 pyrroline-5-carboxylate reductase -
  W01_RS08470 (W01_16560) - 1896347..1896910 (+) 564 WP_173053799.1 YggT family protein -
  W01_RS08475 (W01_16570) - 1896913..1897215 (+) 303 WP_320416777.1 DUF167 family protein -
  W01_RS08480 (W01_16580) - 1897415..1897825 (-) 411 WP_173053803.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42294.81 Da        Isoelectric Point: 5.9288

>NTDB_id=72847 W01_RS08450 WP_173053792.1 1892478..1893614(-) (pilU) [Candidatus Nitrotoga sp. AM1P]
MERDQATELMHNLLRGMITQKASDLFITTGFPPAFKVDGKMTPVSQQVLTPQHTMELANSIMNDRQAAEFEASHECNFAI
SPAGIGRFRVNVFMQQQRIGMVLRTITTKIPDLTQMGMPEVLKDVAMTKRGLVILVGGTGSGKSTTLAAMLGYRNQNTYG
HIITIEDPVEFVHEHINCLITHREVGVDTDSWHVALKNTLRQAPDVIFIGEIRDRETMEYAVTFAETGHLCMATLHANSA
NQALDRIINFFPEERREQLLMDLSLNIKALISQRLIPRVDGIGRSVAMEIMLNSPLISDLIFKGEVLAIKGVMSKSRELG
MQTFDQALFDLHEANLISYEEALKNADSVNDLRLRIKLESQSSKDIDIMAGLDHLQMT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=72847 W01_RS08450 WP_173053792.1 1892478..1893614(-) (pilU) [Candidatus Nitrotoga sp. AM1P]
ATGGAAAGAGATCAGGCTACCGAGCTAATGCATAATCTGTTGCGAGGGATGATTACCCAGAAGGCCTCCGACCTGTTTAT
TACAACCGGATTCCCGCCCGCATTCAAGGTGGACGGCAAGATGACCCCCGTTTCACAACAGGTGCTGACACCACAACACA
CTATGGAATTAGCGAACAGCATCATGAACGACCGTCAAGCCGCTGAATTCGAAGCGTCACACGAATGTAACTTTGCGATC
AGTCCGGCTGGCATTGGCCGCTTCCGCGTTAATGTGTTTATGCAACAGCAGCGTATAGGCATGGTGTTGCGCACCATCAC
CACCAAGATTCCTGATCTTACTCAAATGGGGATGCCAGAAGTACTAAAGGATGTGGCAATGACCAAACGCGGGCTGGTCA
TCCTGGTCGGTGGTACTGGCAGCGGCAAATCTACAACATTGGCAGCCATGCTTGGCTACCGTAACCAGAATACTTACGGT
CACATCATCACTATTGAAGATCCGGTAGAGTTTGTGCATGAGCACATCAACTGCCTCATCACTCACCGCGAAGTTGGGGT
GGACACGGATTCTTGGCACGTCGCATTGAAAAACACGTTGCGTCAAGCGCCGGATGTCATTTTCATCGGTGAGATACGCG
ACCGTGAAACCATGGAATATGCTGTGACGTTTGCCGAGACTGGCCATCTGTGTATGGCCACTCTACACGCTAACAGTGCG
AATCAGGCACTGGATCGCATTATCAACTTCTTCCCGGAAGAGCGTCGCGAGCAATTGTTAATGGATTTGTCTCTCAATAT
CAAGGCACTTATTTCACAGCGCCTAATCCCGCGTGTGGATGGGATAGGCCGTTCAGTGGCCATGGAAATCATGCTCAATT
CACCGCTCATCTCTGATTTGATTTTCAAGGGTGAGGTGCTTGCAATCAAGGGCGTGATGTCTAAATCGCGTGAGCTTGGG
ATGCAGACCTTTGATCAAGCGCTGTTCGATCTGCACGAAGCCAATCTGATCAGTTATGAAGAAGCGCTAAAGAATGCTGA
TTCGGTCAATGACCTGCGCCTGCGTATTAAGCTGGAAAGTCAAAGTTCCAAGGATATCGACATAATGGCGGGGCTGGATC
ACTTGCAGATGACTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.429

92.593

0.569
  pilU Acinetobacter baylyi ADP1

57.103

94.974

0.542
  pilU Vibrio cholerae strain A1552

55.747

92.063

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.776

88.624

0.397
  pilT Pseudomonas aeruginosa PAK

44.648

86.508

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.731

86.508

0.378
  pilT Acinetobacter baylyi ADP1

41.964

88.889

0.373
  pilT Vibrio cholerae strain A1552

43.949

83.069

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.949

83.069

0.365
  pilT Legionella pneumophila strain Lp02

41.818

87.302

0.365
  pilT Legionella pneumophila strain ERS1305867

41.818

87.302

0.365
  pilT Acinetobacter baumannii D1279779

41.818

87.302

0.365
  pilT Acinetobacter baumannii strain A118

41.818

87.302

0.365
  pilT Acinetobacter nosocomialis M2

41.818

87.302

0.365

Multiple sequence alignment