Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NYR97_RS00915 Genome accession   NZ_CP103836
Coordinates   225025..226062 (-) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas hydrangeae strain 22-323     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 220025..231062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR97_RS00890 (NYR97_00890) ruvX 220756..221223 (-) 468 WP_210763159.1 Holliday junction resolvase RuvX -
  NYR97_RS00895 (NYR97_00895) - 221216..221782 (-) 567 WP_006448880.1 YqgE/AlgH family protein -
  NYR97_RS00900 (NYR97_00900) - 222116..222661 (+) 546 WP_316696105.1 DNA-3-methyladenine glycosylase I -
  NYR97_RS00905 (NYR97_00905) - 222802..223476 (-) 675 WP_316698474.1 YitT family protein -
  NYR97_RS00910 (NYR97_00910) pilU 223772..224902 (-) 1131 WP_006448884.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NYR97_RS00915 (NYR97_00915) pilT 225025..226062 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  NYR97_RS00920 (NYR97_00920) - 226603..227319 (+) 717 WP_316696133.1 NPP1 family protein -
  NYR97_RS00925 (NYR97_00925) - 227476..228168 (+) 693 WP_316696134.1 YggS family pyridoxal phosphate-dependent enzyme -
  NYR97_RS00930 (NYR97_00930) proC 228216..229070 (+) 855 WP_316696136.1 pyrroline-5-carboxylate reductase -
  NYR97_RS00935 (NYR97_00935) - 229508..229933 (+) 426 WP_115040574.1 HU family DNA-binding protein -
  NYR97_RS00940 (NYR97_00940) - 230069..230686 (+) 618 WP_316696138.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=725840 NYR97_RS00915 WP_003489919.1 225025..226062(-) (pilT) [Xanthomonas hydrangeae strain 22-323]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=725840 NYR97_RS00915 WP_003489919.1 225025..226062(-) (pilT) [Xanthomonas hydrangeae strain 22-323]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAAAACAAGGCGTCGGACCTGCATCTGTCGGCCGGCTTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGTCGCATCAATATTCCGGCCCTGGACCACAAGCAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAAATTCCGTCGCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGTGCCGGTGCGGTGTTCCGTACCATTCCTTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATTCTCACGGTCGAGGAT
CCGATCGAGTTCGTACACACCTCGCAGAAGTGCCTGATCAATCAGCGCGAAGTGCATCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCCTTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGATCTGGAAACCATCCGCCTGG
CGCTGACCGCTGCGGAAACCGGCCATCTGGTGTTCGGCACCTTGCACACCAGCTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTATTTCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGCGCGGTGATTTCGCAGGC
GTTGTTGAAGAAGGTCGGCGGCGGACGTACCGCCGCCTGGGAAATCATGGTCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGATCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACACGCAACCAGGCGCGCGAGTACGCCAAGGACAAGCGGATATTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394