Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NYR97_RS00910 Genome accession   NZ_CP103836
Coordinates   223772..224902 (-) Length   376 a.a.
NCBI ID   WP_006448884.1    Uniprot ID   A0A0G8P0N2
Organism   Xanthomonas hydrangeae strain 22-323     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 218772..229902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR97_RS00880 (NYR97_00880) - 219156..219590 (-) 435 WP_316696101.1 OsmC family protein -
  NYR97_RS00885 (NYR97_00885) - 219792..220739 (-) 948 WP_205394209.1 aspartate carbamoyltransferase catalytic subunit -
  NYR97_RS00890 (NYR97_00890) ruvX 220756..221223 (-) 468 WP_210763159.1 Holliday junction resolvase RuvX -
  NYR97_RS00895 (NYR97_00895) - 221216..221782 (-) 567 WP_006448880.1 YqgE/AlgH family protein -
  NYR97_RS00900 (NYR97_00900) - 222116..222661 (+) 546 WP_316696105.1 DNA-3-methyladenine glycosylase I -
  NYR97_RS00905 (NYR97_00905) - 222802..223476 (-) 675 WP_316698474.1 YitT family protein -
  NYR97_RS00910 (NYR97_00910) pilU 223772..224902 (-) 1131 WP_006448884.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NYR97_RS00915 (NYR97_00915) pilT 225025..226062 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  NYR97_RS00920 (NYR97_00920) - 226603..227319 (+) 717 WP_316696133.1 NPP1 family protein -
  NYR97_RS00925 (NYR97_00925) - 227476..228168 (+) 693 WP_316696134.1 YggS family pyridoxal phosphate-dependent enzyme -
  NYR97_RS00930 (NYR97_00930) proC 228216..229070 (+) 855 WP_316696136.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41991.26 Da        Isoelectric Point: 6.7684

>NTDB_id=725839 NYR97_RS00910 WP_006448884.1 223772..224902(-) (pilU) [Xanthomonas hydrangeae strain 22-323]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=725839 NYR97_RS00910 WP_006448884.1 223772..224902(-) (pilU) [Xanthomonas hydrangeae strain 22-323]
ATGAGCACTATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCGGGAAT
GCCGCCGGCGATCAAAGTCAATGGCAAGATCAGCCCGATCACCCAGACACCGCTGACTGCACAGCAAAGTCGCGATCTGG
TGTTGAACGTGATGACGCCATCGCAGCGCGAAGAATTCGAAAAGACGCACGAGTGCAACTTCGCCATCGGCGTGTCAGGG
GTCGGGCGTTTTCGTGTGAGCTGTTTCTATCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGCATTCC
CACAGTGGAAGAGTTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATTTTCGTCGGCG
CCACCGGTACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCGACCGGGCACATCATCACC
ATCGAAGATCCGATCGAATTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAAAACGCTCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGATCACGCCGTGGCCTTTGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGATCGCCGCAACCAACTGCTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAATTGATTCCGACGCCGGACGGGCGCAGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACCCCGCTGGTGC
AGGATTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTGTATCAGGCCGGCGAAATCAGCTACGAAGACGCGCTGCGTTACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAACTCTCGCAGGGCGGCGACGCCAAGACGCTGGCGCAGGGGATGGATGGTGTGGAGATTGCCG
AGGTTCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8P0N2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.667

98.138

0.654
  pilU Acinetobacter baylyi ADP1

64.426

94.947

0.612
  pilU Vibrio cholerae strain A1552

53.371

94.681

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364