Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M52SOB_RS12355 Genome accession   NZ_AP019373
Coordinates   2447753..2448796 (-) Length   347 a.a.
NCBI ID   WP_131112085.1    Uniprot ID   -
Organism   Sulfuricystis thermophila strain M52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2442753..2453796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M52SOB_RS12330 - 2443290..2444174 (+) 885 WP_131112080.1 NAD-dependent epimerase/dehydratase family protein -
  M52SOB_RS12335 - 2444162..2444578 (+) 417 WP_131112081.1 CopD family protein -
  M52SOB_RS12340 - 2444911..2446098 (+) 1188 WP_284155108.1 THUMP domain-containing protein -
  M52SOB_RS12345 - 2446102..2446590 (-) 489 WP_131112083.1 hypothetical protein -
  M52SOB_RS12350 pilU 2446611..2447747 (-) 1137 WP_131112084.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M52SOB_RS12355 pilT 2447753..2448796 (-) 1044 WP_131112085.1 type IV pilus twitching motility protein PilT Machinery gene
  M52SOB_RS12360 - 2448864..2449553 (+) 690 WP_131112086.1 YggS family pyridoxal phosphate-dependent enzyme -
  M52SOB_RS12365 proC 2449550..2450362 (+) 813 WP_131112087.1 pyrroline-5-carboxylate reductase -
  M52SOB_RS12370 - 2450362..2450907 (+) 546 WP_131112088.1 YggT family protein -
  M52SOB_RS12375 - 2450907..2451197 (+) 291 WP_131112089.1 DUF167 family protein -
  M52SOB_RS12380 - 2451198..2452034 (+) 837 WP_131112090.1 symmetrical bis(5'-nucleosyl)-tetraphosphatase -
  M52SOB_RS13980 - 2452400..2452975 (+) 576 WP_172601842.1 C-type lectin domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38210.03 Da        Isoelectric Point: 7.1070

>NTDB_id=72509 M52SOB_RS12355 WP_131112085.1 2447753..2448796(-) (pilT) [Sulfuricystis thermophila strain M52]
MDITELLAFAVKNKASDLHLSSGLPPMIRVHGDIRRINLPPMEHKDVHAMVYDIMNDAQRKHYEETLECDFSFAVPNLAR
FRVNAYVQNRGAAAAFRTIPSKVLTLEQLNAPKIFAEIADQPRGIVLVTGPTGSGKSTTLAAMIDHINEKEFAHILTVED
PIEFVHESKKCLINQREVGPHTLSFANALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRSMLSESLRAVIAQTLLKTKDGNGRVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTGQQFGMQTLDQC
LADLVKRNIVSAAEARAKAANKDSFLG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=72509 M52SOB_RS12355 WP_131112085.1 2447753..2448796(-) (pilT) [Sulfuricystis thermophila strain M52]
ATGGACATCACTGAATTGCTGGCGTTTGCCGTCAAGAACAAGGCCTCGGACCTGCACCTGTCTTCCGGTCTGCCGCCGAT
GATCCGCGTGCACGGTGACATCCGGCGCATCAACCTGCCGCCGATGGAGCACAAGGACGTGCATGCGATGGTCTATGACA
TCATGAACGATGCCCAGCGCAAGCACTACGAAGAGACGCTGGAATGCGACTTCTCGTTCGCGGTGCCCAATCTGGCGCGC
TTCCGCGTCAATGCCTACGTGCAGAACCGCGGTGCGGCGGCGGCGTTCCGCACCATTCCCTCGAAGGTGCTCACCCTCGA
ACAGCTCAACGCGCCGAAGATTTTCGCCGAGATCGCCGACCAGCCGCGCGGCATCGTGCTGGTCACCGGGCCGACCGGCT
CCGGCAAATCGACGACACTGGCGGCGATGATCGATCACATCAACGAAAAGGAATTCGCCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCATGAGTCGAAGAAGTGCCTGATCAACCAACGCGAAGTCGGACCGCACACGTTGTCGTTCGCCAA
TGCCCTGCGCTCGGCGCTGCGCGAGGATCCGGACGTGATCCTCGTCGGCGAAATGCGCGACCTGGAAACCATTCGCCTGG
CGCTCACCGCCGCCGAAACCGGCCACCTGGTGTTCGGCACGCTGCATACCTCGTCGGCGGCCAAGACCATCGACCGGATC
GTCGACGTCTTCCCCGCGGCGGAAAAGGAGATGGTGCGTTCGATGTTGTCCGAATCCTTGCGCGCGGTGATCGCGCAGAC
GCTGTTGAAGACCAAGGACGGCAATGGCCGGGTGGCTGCACACGAGATCATGATCGGCACACCGGCGATCCGCAACCTGA
TCCGCGAGAACAAGGTCGCGCAAATGTATTCGGCGATCCAGACCGGCCAGCAATTCGGCATGCAAACCCTCGACCAGTGT
CTGGCGGATCTGGTCAAGCGCAACATCGTCTCGGCGGCCGAGGCGCGCGCCAAGGCGGCCAACAAGGATAGTTTCCTCGG
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.168

99.712

0.769
  pilT Acinetobacter baumannii D1279779

77.168

99.712

0.769
  pilT Acinetobacter baumannii strain A118

77.168

99.712

0.769
  pilT Pseudomonas aeruginosa PAK

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Legionella pneumophila strain Lp02

72.754

99.424

0.723
  pilT Legionella pneumophila strain ERS1305867

72.754

99.424

0.723
  pilT Neisseria meningitidis 8013

72.464

99.424

0.72
  pilT Neisseria gonorrhoeae MS11

72.174

99.424

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.639

97.406

0.669
  pilT Vibrio cholerae strain A1552

68.639

97.406

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

99.424

0.516
  pilU Vibrio cholerae strain A1552

41.108

98.847

0.406
  pilU Acinetobacter baylyi ADP1

41.124

97.406

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.542

0.392

Multiple sequence alignment