Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M52SOB_RS12350 Genome accession   NZ_AP019373
Coordinates   2446611..2447747 (-) Length   378 a.a.
NCBI ID   WP_131112084.1    Uniprot ID   -
Organism   Sulfuricystis thermophila strain M52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2441611..2452747
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M52SOB_RS12325 - 2442271..2443275 (-) 1005 WP_131112079.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  M52SOB_RS12330 - 2443290..2444174 (+) 885 WP_131112080.1 NAD-dependent epimerase/dehydratase family protein -
  M52SOB_RS12335 - 2444162..2444578 (+) 417 WP_131112081.1 CopD family protein -
  M52SOB_RS12340 - 2444911..2446098 (+) 1188 WP_284155108.1 THUMP domain-containing protein -
  M52SOB_RS12345 - 2446102..2446590 (-) 489 WP_131112083.1 hypothetical protein -
  M52SOB_RS12350 pilU 2446611..2447747 (-) 1137 WP_131112084.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M52SOB_RS12355 pilT 2447753..2448796 (-) 1044 WP_131112085.1 type IV pilus twitching motility protein PilT Machinery gene
  M52SOB_RS12360 - 2448864..2449553 (+) 690 WP_131112086.1 YggS family pyridoxal phosphate-dependent enzyme -
  M52SOB_RS12365 proC 2449550..2450362 (+) 813 WP_131112087.1 pyrroline-5-carboxylate reductase -
  M52SOB_RS12370 - 2450362..2450907 (+) 546 WP_131112088.1 YggT family protein -
  M52SOB_RS12375 - 2450907..2451197 (+) 291 WP_131112089.1 DUF167 family protein -
  M52SOB_RS12380 - 2451198..2452034 (+) 837 WP_131112090.1 symmetrical bis(5'-nucleosyl)-tetraphosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42153.70 Da        Isoelectric Point: 6.5062

>NTDB_id=72508 M52SOB_RS12350 WP_131112084.1 2446611..2447747(-) (pilU) [Sulfuricystis thermophila strain M52]
MERDESLKFLYQLLAMLVQKKGSDLFITAGFPPAIKVDGKVTPVTAQPLMPQHTIELAHAVMNDKQAATFEETKECNFAI
SPAGIGRFRVNAFIQQGRVGLVFRTINTAIPKFEDLGLPPILKDIGMTKRGLVLFVGGTGSGKSTSLAAMIGYRNEHSYG
HIITIEDPVEYVHEHKNCIITQREVGVDTDSWEAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCMGTLHANNA
NQAMDRIINFFPEERRPQLLMDLSLNLKAVVSQRLLPAREGKGRVAAVEILINSPLISDLIGKGEVHEIKEVMKRSRELG
MQTFDQALFDLYEAGKISYEDALRFADSMNEVRLQIKLHGKEMAERDLQSGIKHLDII

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=72508 M52SOB_RS12350 WP_131112084.1 2446611..2447747(-) (pilU) [Sulfuricystis thermophila strain M52]
ATGGAACGCGACGAATCGCTCAAATTCCTCTACCAGCTCTTGGCGATGCTGGTGCAGAAGAAGGGCTCGGACCTGTTCAT
TACCGCCGGCTTTCCACCGGCGATCAAGGTCGACGGCAAGGTGACACCGGTCACCGCGCAGCCGCTGATGCCCCAGCACA
CCATCGAACTGGCGCACGCGGTGATGAACGACAAGCAGGCGGCCACCTTCGAGGAAACCAAGGAGTGCAACTTCGCGATC
TCGCCAGCCGGCATCGGCAGATTCCGTGTCAATGCCTTCATCCAGCAGGGGCGCGTCGGCCTGGTGTTCCGCACCATCAA
CACCGCGATCCCGAAGTTCGAGGATCTCGGCCTGCCGCCCATCCTCAAGGACATCGGCATGACCAAGCGCGGCCTGGTGC
TGTTCGTCGGCGGCACCGGTTCTGGCAAGTCGACTTCGCTGGCGGCGATGATCGGCTACCGCAACGAACACAGCTACGGT
CACATCATCACGATCGAGGATCCGGTCGAATACGTGCATGAGCACAAGAACTGCATCATCACCCAGCGCGAGGTCGGCGT
CGATACCGATTCCTGGGAGGCGGCACTGAAGAATACGTTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCG
ACCGCGAGACGATGGAACATGCGATCGCCTTTGCCGAGACCGGCCACCTGTGCATGGGCACGCTGCACGCCAACAACGCC
AACCAGGCGATGGACCGCATCATCAACTTCTTCCCCGAAGAGCGGCGCCCGCAGCTCTTGATGGATCTGTCGCTGAACCT
CAAGGCCGTCGTTTCGCAGCGGCTGCTGCCGGCGCGTGAAGGCAAGGGGCGTGTCGCGGCGGTCGAGATTCTGATCAACT
CGCCGCTGATCTCCGATCTGATCGGCAAGGGCGAGGTGCACGAGATCAAGGAAGTGATGAAGCGTTCGCGCGAGCTTGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGATCTTTATGAAGCCGGCAAGATCAGCTATGAGGATGCGCTCCGCTTCGCCGA
CTCGATGAACGAAGTGCGCCTGCAGATCAAGCTGCACGGCAAGGAAATGGCCGAGCGCGATCTGCAAAGCGGCATCAAGC
ATCTCGACATCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.435

91.799

0.619
  pilU Acinetobacter baylyi ADP1

61.671

91.799

0.566
  pilU Vibrio cholerae strain A1552

57.759

92.063

0.532
  pilT Acinetobacter baylyi ADP1

46.313

89.683

0.415
  pilT Legionella pneumophila strain Lp02

46.547

88.095

0.41
  pilT Legionella pneumophila strain ERS1305867

46.547

88.095

0.41
  pilT Acinetobacter baumannii strain A118

46.246

88.095

0.407
  pilT Acinetobacter nosocomialis M2

46.246

88.095

0.407
  pilT Acinetobacter baumannii D1279779

46.246

88.095

0.407
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

45.345

88.095

0.399
  pilT Pseudomonas stutzeri DSM 10701

45.045

88.095

0.397
  pilT Neisseria meningitidis 8013

42.353

89.947

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.072

87.831

0.378
  pilT Vibrio cholerae strain A1552

43.072

87.831

0.378
  pilT Neisseria gonorrhoeae MS11

41.888

89.683

0.376

Multiple sequence alignment