Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GYH29_RS23020 Genome accession   NZ_AP019312
Coordinates   4973833..4974870 (-) Length   345 a.a.
NCBI ID   WP_043589321.1    Uniprot ID   -
Organism   Chromobacterium haemolyticum strain CH06-BL     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4968833..4979870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYH29_RS23000 (CH06BL_45450) - 4968937..4969644 (-) 708 WP_019100948.1 pseudouridine synthase -
  GYH29_RS23005 (CH06BL_45460) - 4969686..4971602 (-) 1917 WP_161524452.1 diguanylate cyclase -
  GYH29_RS23010 (CH06BL_45470) - 4971932..4972519 (+) 588 WP_043589326.1 TetR/AcrR family transcriptional regulator -
  GYH29_RS23015 (CH06BL_45480) pilU 4972651..4973787 (-) 1137 WP_043589323.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GYH29_RS23020 (CH06BL_45490) pilT 4973833..4974870 (-) 1038 WP_043589321.1 type IV pilus twitching motility protein PilT Machinery gene
  GYH29_RS23025 (CH06BL_45500) - 4975146..4975838 (+) 693 WP_039755535.1 YggS family pyridoxal phosphate-dependent enzyme -
  GYH29_RS23030 (CH06BL_45510) proC 4975892..4976695 (+) 804 WP_019102523.1 pyrroline-5-carboxylate reductase -
  GYH29_RS23035 (CH06BL_45520) - 4976704..4977285 (+) 582 WP_019102524.1 YggT family protein -
  GYH29_RS23040 (CH06BL_45530) - 4977376..4977681 (+) 306 WP_019102525.1 c-type cytochrome -
  GYH29_RS23045 (CH06BL_45540) dksA 4977966..4978376 (+) 411 WP_019102526.1 RNA polymerase-binding protein DksA -
  GYH29_RS23050 (CH06BL_45550) aroG 4978608..4979669 (+) 1062 WP_019102527.1 3-deoxy-7-phosphoheptulonate synthase AroG -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38336.12 Da        Isoelectric Point: 6.7774

>NTDB_id=72395 GYH29_RS23020 WP_043589321.1 4973833..4974870(-) (pilT) [Chromobacterium haemolyticum strain CH06-BL]
MEISELLAFTVKNKASDLHLSAGLPPMIRVNGDIRRINLPPMDHHDVHDMVYDIMNDYQRKIFEDTFECDFSFDLPGIAR
FRVNSFVQNRGMGAVFRVIPSKVLSLEQLGAPKIFQEIAAFPRGLVLVTGPTGSGKSTTLAAMIDFINDNHFSHILTVED
PIEFVHDSKKCLINQRELGLQTHSFANALKSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAGEKEMVRSMLSESVRAVIAQTLLKTKDGSGRVAAHEIMLGTPAIRNLIRENKIAQINSMIQTGQQHGMQTLDQC
LQELVRRNMVSPADARQKAANKDQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=72395 GYH29_RS23020 WP_043589321.1 4973833..4974870(-) (pilT) [Chromobacterium haemolyticum strain CH06-BL]
ATGGAAATTTCGGAGCTCTTGGCCTTTACGGTCAAGAACAAGGCGTCCGACCTGCACTTGTCCGCCGGGCTGCCGCCGAT
GATCCGGGTAAACGGCGACATCCGCCGCATCAACCTGCCGCCGATGGACCACCATGACGTGCACGACATGGTGTACGACA
TCATGAACGATTACCAGCGCAAAATCTTCGAAGACACCTTCGAGTGCGACTTTTCTTTCGATCTGCCCGGCATCGCCCGT
TTCCGAGTCAACTCCTTCGTGCAGAACCGCGGCATGGGCGCGGTGTTTCGGGTGATTCCGTCCAAGGTGTTGTCGCTGGA
ACAGTTGGGCGCGCCCAAGATATTCCAGGAAATCGCCGCTTTTCCGCGCGGGCTGGTGCTGGTGACCGGCCCCACCGGCT
CCGGCAAGTCCACTACGCTGGCGGCGATGATAGATTTCATCAACGACAACCACTTCTCCCACATCCTGACTGTCGAGGAT
CCGATCGAGTTCGTCCACGACAGCAAGAAGTGCCTGATCAACCAGCGTGAACTGGGTTTGCAGACACACAGCTTCGCCAA
TGCCTTGAAGTCCGCCTTGCGGGAAGACCCGGATGTGATCCTGGTGGGCGAACTGCGCGATCTGGAAACCATACGCTTGG
CGCTGACCGCGGCGGAAACCGGTCACTTGGTGTTCGGCACGCTGCACACCAGTTCCGCGGCCAAGACCATAGACCGCATT
GTCGACGTATTCCCTGCCGGGGAAAAGGAGATGGTCCGTTCCATGCTGTCCGAGTCGGTGCGCGCGGTGATCGCCCAAAC
CCTGCTCAAAACCAAGGACGGCAGCGGTCGGGTGGCGGCGCATGAAATCATGCTGGGCACCCCGGCGATCCGCAACTTGA
TCCGCGAGAACAAGATTGCTCAAATCAACTCCATGATCCAGACCGGCCAGCAGCATGGGATGCAGACGCTGGACCAGTGC
CTGCAGGAGCTGGTGCGGCGCAATATGGTGTCGCCGGCGGACGCCAGGCAAAAGGCCGCCAACAAGGATCAATTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

72.174

100

0.722
  pilT Acinetobacter baumannii strain A118

72.174

100

0.722
  pilT Acinetobacter baumannii D1279779

72.174

100

0.722
  pilT Neisseria meningitidis 8013

71.429

99.42

0.71
  pilT Neisseria gonorrhoeae MS11

71.429

99.42

0.71
  pilT Pseudomonas stutzeri DSM 10701

71.014

100

0.71
  pilT Legionella pneumophila strain Lp02

70.725

100

0.707
  pilT Pseudomonas aeruginosa PAK

70.725

100

0.707
  pilT Legionella pneumophila strain ERS1305867

70.725

100

0.707
  pilT Acinetobacter baylyi ADP1

70.435

100

0.704
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.568

97.971

0.652
  pilT Vibrio cholerae strain A1552

66.568

97.971

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.888

98.261

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.101

0.409
  pilU Acinetobacter baylyi ADP1

41.176

98.551

0.406

Multiple sequence alignment