Detailed information NTG0723806

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: M5S90_RS04210
• Genome accession: NZ_CP103633
• Coordinates: 886077..887057 (+)
• Length: 326 a.a.
• NCBI ID: WP_072023908.1
• Uniprot ID: -
• Organism: Escherichia coli strain 2900
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 881077..892057

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5S90_RS04180 (M5S90_04180)	yggM	881678..882685 (+)	1008	WP_032152713.1	DUF1202 family protein	-
M5S90_RS04185 (M5S90_04185)	hemW	882754..883890 (-)	1137	WP_032152712.1	radical SAM family heme chaperone HemW	-
M5S90_RS04190 (M5S90_04190)	rdgB	883883..884476 (-)	594	WP_001174738.1	XTP/dITP diphosphatase	-
M5S90_RS04195 (M5S90_04195)	yggU	884484..884774 (-)	291	WP_001277229.1	DUF167 family protein YggU	-
M5S90_RS04200 (M5S90_04200)	yggT	884771..885337 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
M5S90_RS04205 (M5S90_04205)	yggS	885355..886059 (-)	705	WP_032152711.1	pyridoxal phosphate homeostasis protein	-
M5S90_RS04210 (M5S90_04210)	pilT	886077..887057 (+)	981	WP_072023908.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
M5S90_RS04215 (M5S90_04215)	ruvX	887172..887588 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
M5S90_RS04220 (M5S90_04220)	yqgE	887588..888151 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
M5S90_RS04225 (M5S90_04225)	gshB	888260..889210 (-)	951	WP_000593273.1	glutathione synthase	-
M5S90_RS04230 (M5S90_04230)	rsmE	889223..889954 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
M5S90_RS04235 (M5S90_04235)	endA	890034..890741 (-)	708	WP_000286497.1	deoxyribonuclease I	-
M5S90_RS04240 (M5S90_04240)	yggI	890836..891333 (-)	498	WP_032152710.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35958.12 Da
• Isoelectric Point: 5.5860

>NTDB_id=723806 M5S90_RS04210 WP_072023908.1 886077..887057(+) (pilT) [Escherichia coli strain 2900]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQW
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=723806 M5S90_RS04210 WP_072023908.1 886077..887057(+) (pilT) [Escherichia coli strain 2900]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTCGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCGTTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTAACAG
CGGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCAGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGTGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.177	100	0.463
pilT	Acinetobacter baumannii D1279779	46.177	100	0.463
pilT	Acinetobacter nosocomialis M2	46.177	100	0.463
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	45.26	100	0.454
pilT	Legionella pneumophila strain Lp02	45.26	100	0.454
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	38.037	100	0.38
pilU	Acinetobacter baylyi ADP1	36.646	98.773	0.362
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362