Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5T00_RS04385 Genome accession   NZ_CP103589
Coordinates   902738..903718 (+) Length   326 a.a.
NCBI ID   WP_001305314.1    Uniprot ID   S1P1E1
Organism   Escherichia coli strain 4750     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 897738..908718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T00_RS04355 (M5T00_04355) yggM 898343..899350 (+) 1008 WP_000745234.1 DUF1202 family protein -
  M5T00_RS04360 (M5T00_04360) hemW 899415..900551 (-) 1137 WP_000239983.1 radical SAM family heme chaperone HemW -
  M5T00_RS04365 (M5T00_04365) rdgB 900544..901137 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  M5T00_RS04370 (M5T00_04370) yggU 901145..901435 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  M5T00_RS04375 (M5T00_04375) yggT 901432..901998 (-) 567 WP_001094838.1 osmotic shock tolerance protein YggT -
  M5T00_RS04380 (M5T00_04380) yggS 902016..902720 (-) 705 WP_000997805.1 pyridoxal phosphate homeostasis protein -
  M5T00_RS04385 (M5T00_04385) pilT 902738..903718 (+) 981 WP_001305314.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M5T00_RS04390 (M5T00_04390) ruvX 903892..904308 (-) 417 WP_000017107.1 Holliday junction resolvase RuvX -
  M5T00_RS04395 (M5T00_04395) yqgE 904308..904871 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  M5T00_RS04400 (M5T00_04400) gshB 904980..905930 (-) 951 WP_000593255.1 glutathione synthase -
  M5T00_RS04405 (M5T00_04405) rsmE 905943..906674 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M5T00_RS04410 (M5T00_04410) endA 906754..907461 (-) 708 WP_001305312.1 deoxyribonuclease I -
  M5T00_RS04415 (M5T00_04415) yggI 907556..908053 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36043.27 Da        Isoelectric Point: 5.9975

>NTDB_id=723556 M5T00_RS04385 WP_001305314.1 902738..903718(+) (pilT) [Escherichia coli strain 4750]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=723556 M5T00_RS04385 WP_001305314.1 902738..903718(+) (pilT) [Escherichia coli strain 4750]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S1P1E1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362