Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5T49_RS04585 Genome accession   NZ_CP103557
Coordinates   930796..931776 (+) Length   326 a.a.
NCBI ID   WP_001296360.1    Uniprot ID   A0A1L3YZP2
Organism   Escherichia coli O25b:H4-ST131 strain 4993     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 925796..936776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T49_RS04555 (M5T49_04555) - 926172..927158 (-) 987 WP_000783999.1 TRAP transporter substrate-binding protein -
  M5T49_RS04560 (M5T49_04560) hemW 927473..928609 (-) 1137 WP_000239963.1 radical SAM family heme chaperone HemW -
  M5T49_RS04565 (M5T49_04565) rdgB 928602..929195 (-) 594 WP_001174754.1 XTP/dITP diphosphatase -
  M5T49_RS04570 (M5T49_04570) yggU 929203..929493 (-) 291 WP_001277229.1 DUF167 family protein YggU -
  M5T49_RS04575 (M5T49_04575) yggT 929490..930056 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  M5T49_RS04580 (M5T49_04580) yggS 930074..930778 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  M5T49_RS04585 (M5T49_04585) pilT 930796..931776 (+) 981 WP_001296360.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M5T49_RS04590 (M5T49_04590) ruvX 931891..932307 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  M5T49_RS04595 (M5T49_04595) yqgE 932307..932870 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  M5T49_RS04600 (M5T49_04600) gshB 932979..933929 (-) 951 WP_000593274.1 glutathione synthase -
  M5T49_RS04605 (M5T49_04605) rsmE 933942..934673 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M5T49_RS04610 (M5T49_04610) endA 934753..935460 (-) 708 WP_000286500.1 deoxyribonuclease I -
  M5T49_RS04615 (M5T49_04615) yggI 935555..936052 (-) 498 WP_001296359.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35972.15 Da        Isoelectric Point: 5.7980

>NTDB_id=723348 M5T49_RS04585 WP_001296360.1 930796..931776(+) (pilT) [Escherichia coli O25b:H4-ST131 strain 4993]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=723348 M5T49_RS04585 WP_001296360.1 930796..931776(+) (pilT) [Escherichia coli O25b:H4-ST131 strain 4993]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGTTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
TGCCGCATTGCGGGAAGATCCCGATGTGATATTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGGCTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCGGGGAGTTTACGGGCAGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1L3YZP2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362