Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SHINM1_RS11155 Genome accession   NZ_AP019011
Coordinates   2221402..2222427 (+) Length   341 a.a.
NCBI ID   WP_162050795.1    Uniprot ID   A0A679I0A8
Organism   Fluviibacter phosphoraccumulans strain SHINM1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2216402..2227427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHINM1_RS11125 (SHINM1_021930) pyrR 2216709..2217233 (+) 525 WP_162050801.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  SHINM1_RS11130 (SHINM1_021940) - 2217226..2218185 (+) 960 WP_162050800.1 aspartate carbamoyltransferase catalytic subunit -
  SHINM1_RS11135 (SHINM1_021950) - 2218187..2219452 (+) 1266 WP_162050799.1 dihydroorotase -
  SHINM1_RS11140 (SHINM1_021960) - 2219453..2219773 (-) 321 WP_242451471.1 DUF167 domain-containing protein -
  SHINM1_RS11145 (SHINM1_021970) proC 2219766..2220578 (-) 813 WP_162050797.1 pyrroline-5-carboxylate reductase -
  SHINM1_RS11150 (SHINM1_021980) - 2220575..2221309 (-) 735 WP_162050796.1 YggS family pyridoxal phosphate-dependent enzyme -
  SHINM1_RS11155 (SHINM1_021990) pilT 2221402..2222427 (+) 1026 WP_162050795.1 type IV pilus twitching motility protein PilT Machinery gene
  SHINM1_RS11160 (SHINM1_022000) - 2222430..2222648 (+) 219 WP_162050794.1 type II toxin-antitoxin system MqsA family antitoxin -
  SHINM1_RS11165 (SHINM1_022010) - 2222963..2224309 (+) 1347 WP_162050793.1 hypothetical protein -
  SHINM1_RS11170 - 2224362..2224913 (+) 552 WP_162050792.1 DUF1643 domain-containing protein -
  SHINM1_RS11175 (SHINM1_022020) - 2224925..2226001 (-) 1077 WP_162050791.1 hypothetical protein -
  SHINM1_RS11180 (SHINM1_022030) - 2226001..2226708 (-) 708 WP_162050790.1 uracil-DNA glycosylase family protein -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 36867.56 Da        Isoelectric Point: 5.4096

>NTDB_id=72087 SHINM1_RS11155 WP_162050795.1 2221402..2222427(+) (pilT) [Fluviibacter phosphoraccumulans strain SHINM1]
MLESLLLIARDLGASDIHLSAGMPPMLRINGDLQPLDQPVLGNAQVRGMIDSVIPETLRRLFGEGAACDFAFASPMEGLG
RYRANAYRQQRGVALALRPIPDKVPTLAEIAAPPILQQLAEIDHGLVLVVGPTGSGKSTTLAAMANHINQTAPKHLLTIE
DPVEFVYEARQCLLTQREVGRDTPSFADALKAALREDPDVILLGEMRDLETIRLALTAAETGHLVLATLHTATAPQAIER
IVDVFPGEERDLARTLLADVLRAVVAQQLLARNDGQGRVAAHEILVGTPAVRNLIRENKTAQLVSVMQTGQQQGMQTMAQ
SLERLTQAGLVYICAEGFRQL

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=72087 SHINM1_RS11155 WP_162050795.1 2221402..2222427(+) (pilT) [Fluviibacter phosphoraccumulans strain SHINM1]
ATGCTTGAATCATTACTCTTGATCGCCCGCGATTTGGGGGCTTCCGATATCCACCTGTCGGCCGGCATGCCGCCAATGCT
GCGTATTAACGGTGATTTGCAACCTCTGGATCAACCGGTACTGGGTAATGCGCAAGTGCGCGGCATGATTGACAGCGTCA
TCCCGGAAACCCTGCGCCGTTTGTTCGGTGAAGGCGCTGCTTGCGATTTTGCATTTGCATCGCCTATGGAGGGCTTGGGG
CGTTACCGTGCCAATGCCTACCGTCAGCAACGTGGCGTTGCATTAGCCTTGCGACCTATTCCTGACAAAGTGCCTACCCT
GGCCGAAATTGCAGCACCGCCCATCCTGCAACAATTAGCAGAGATCGATCATGGGCTGGTGTTGGTCGTCGGCCCAACCG
GTTCTGGTAAAAGCACCACACTGGCCGCCATGGCGAACCATATTAATCAGACAGCCCCTAAACACTTGCTGACCATTGAA
GACCCGGTGGAGTTTGTTTATGAAGCCCGGCAGTGTCTCCTTACACAACGTGAAGTCGGTCGCGACACCCCCAGCTTCGC
GGATGCGCTCAAAGCAGCGCTACGAGAAGACCCTGATGTCATCTTGCTGGGCGAAATGCGTGACCTGGAAACCATTCGCC
TGGCGCTGACAGCCGCAGAAACCGGCCACCTCGTTCTGGCAACGCTGCATACAGCGACAGCCCCTCAGGCCATCGAACGT
ATCGTTGATGTGTTCCCTGGGGAAGAACGGGATCTGGCCCGCACCTTATTGGCCGATGTGCTTCGTGCCGTCGTCGCGCA
GCAGTTATTAGCGCGTAATGATGGGCAAGGTCGGGTAGCCGCTCACGAAATTCTGGTCGGTACCCCAGCCGTTCGTAATC
TCATTAGAGAGAACAAAACGGCTCAACTCGTCTCGGTGATGCAGACAGGGCAACAGCAAGGTATGCAGACTATGGCGCAG
AGCCTTGAAAGGCTAACCCAGGCTGGTTTGGTTTATATTTGCGCCGAAGGTTTTAGGCAGTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A679I0A8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

53.823

95.894

0.516
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.823

95.894

0.516
  pilT Vibrio cholerae strain A1552

53.823

95.894

0.516
  pilT Neisseria meningitidis 8013

54.717

93.255

0.51
  pilT Pseudomonas aeruginosa PAK

52.424

96.774

0.507
  pilT Pseudomonas stutzeri DSM 10701

52.121

96.774

0.504
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.818

96.774

0.501
  pilT Acinetobacter baumannii strain A118

51.515

96.774

0.499
  pilT Acinetobacter nosocomialis M2

51.515

96.774

0.499
  pilT Acinetobacter baumannii D1279779

51.515

96.774

0.499
  pilT Acinetobacter baylyi ADP1

53.145

93.255

0.496
  pilT Legionella pneumophila strain Lp02

51.887

93.255

0.484
  pilT Legionella pneumophila strain ERS1305867

51.887

93.255

0.484
  pilU Pseudomonas stutzeri DSM 10701

41.768

96.188

0.402
  pilU Vibrio cholerae strain A1552

41.987

91.496

0.384
  pilU Acinetobacter baylyi ADP1

41.748

90.616

0.378

Multiple sequence alignment