Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NWF24_RS01385 Genome accession   NZ_CP102931
Coordinates   290846..291889 (-) Length   347 a.a.
NCBI ID   WP_007833310.1    Uniprot ID   J2KQ44
Organism   Variovorax paradoxus strain JBCE486     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 285846..296889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NWF24_RS01365 (NWF24_01365) - 286638..287546 (-) 909 WP_258352672.1 NAD(P)-dependent oxidoreductase -
  NWF24_RS01370 (NWF24_01370) - 287634..288116 (+) 483 WP_258352673.1 MerR family transcriptional regulator -
  NWF24_RS01375 (NWF24_01375) - 288113..289663 (+) 1551 WP_258352674.1 MFS transporter -
  NWF24_RS01380 (NWF24_01380) pilU 289675..290811 (-) 1137 WP_097197835.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NWF24_RS01385 (NWF24_01385) pilT 290846..291889 (-) 1044 WP_007833310.1 type IV pilus twitching motility protein PilT Machinery gene
  NWF24_RS01390 (NWF24_01390) - 291941..292642 (+) 702 WP_258355407.1 YggS family pyridoxal phosphate-dependent enzyme -
  NWF24_RS01395 (NWF24_01395) - 292631..293098 (-) 468 WP_258352675.1 Lrp/AsnC ligand binding domain-containing protein -
  NWF24_RS01400 (NWF24_01400) - 293249..293707 (-) 459 WP_258352676.1 hypothetical protein -
  NWF24_RS01405 (NWF24_01405) - 293948..295222 (+) 1275 WP_258352677.1 Glu/Leu/Phe/Val dehydrogenase -
  NWF24_RS01410 (NWF24_01410) - 295259..295912 (+) 654 WP_258352678.1 nucleoside/nucleotide kinase family protein -
  NWF24_RS01415 (NWF24_01415) - 295995..296690 (+) 696 WP_258352679.1 DUF2242 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38134.80 Da        Isoelectric Point: 6.8356

>NTDB_id=719818 NWF24_RS01385 WP_007833310.1 290846..291889(-) (pilT) [Variovorax paradoxus strain JBCE486]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKGVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGSGMQTLDQN
LTELVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=719818 NWF24_RS01385 WP_007833310.1 290846..291889(-) (pilT) [Variovorax paradoxus strain JBCE486]
GTGGACATCACCCAATTGCTGGCGTTCAGCGTCAAGAACAAAGCCTCCGACCTGCATCTTTCCGCAGGCCTGCCGCCCAT
GATCCGCGTGAACGGCGACGTGCGCCGCATCAACGTCGACGCACTCGATCACAAGGGCGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCGTTCGAGATCGACGGCCTCGCGCGC
TTCCGCGTGAACGCCTTCAACCAGGCACGTGGCGCGGCCGCGGTGTTCCGGACCATTCCCTCGAAGATCCTGACGCTGGA
GCAGCTGAACGCACCGAAGATTTTCGGCGAACTCGCGCTCAAGCCGCGCGGGCTGGTGCTGGTGACGGGCCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCCGCCATGGTCAACTACCTCAACGAGAACGAATACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCGATGACGCTGTCGTTCTCGAA
CGCGCTGCGCTCCGCCCTGCGCGAAGACCCGGACGCCATCCTGGTGGGCGAGTTGCGCGACCTCGAAACCATCCGCCTCG
CCATGACCGCGGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCTTCCGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCCGGCGAAGAAAAGGAAATGATCCGCGCAATGCTGTCGGAATCACTGCAGGCAGTGATCTCGCAGAC
GCTGTGTAAGACCAAGGACGGCCAGGGCCGCGTGGCAGCGCACGAGATCATGCTGGGCACGCCCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCTCGGGCATGCAGACGCTCGACCAGAAC
CTGACAGAGCTGGTGCGCCGCAATACCATCTCGGCAGCCGAAGCCCGCGGCAAGGCGAAGATCCCCGAAAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J2KQ44

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.915

98.271

0.677
  pilT Vibrio cholerae strain A1552

68.915

98.271

0.677
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398