Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NWF24_RS01380 Genome accession   NZ_CP102931
Coordinates   289675..290811 (-) Length   378 a.a.
NCBI ID   WP_097197835.1    Uniprot ID   A0A286ANQ1
Organism   Variovorax paradoxus strain JBCE486     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 284675..295811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NWF24_RS01355 (NWF24_01355) - 285127..285771 (+) 645 WP_093057690.1 SIS domain-containing protein -
  NWF24_RS01360 (NWF24_01360) - 285768..286586 (+) 819 WP_258352671.1 BON domain-containing protein -
  NWF24_RS01365 (NWF24_01365) - 286638..287546 (-) 909 WP_258352672.1 NAD(P)-dependent oxidoreductase -
  NWF24_RS01370 (NWF24_01370) - 287634..288116 (+) 483 WP_258352673.1 MerR family transcriptional regulator -
  NWF24_RS01375 (NWF24_01375) - 288113..289663 (+) 1551 WP_258352674.1 MFS transporter -
  NWF24_RS01380 (NWF24_01380) pilU 289675..290811 (-) 1137 WP_097197835.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NWF24_RS01385 (NWF24_01385) pilT 290846..291889 (-) 1044 WP_007833310.1 type IV pilus twitching motility protein PilT Machinery gene
  NWF24_RS01390 (NWF24_01390) - 291941..292642 (+) 702 WP_258355407.1 YggS family pyridoxal phosphate-dependent enzyme -
  NWF24_RS01395 (NWF24_01395) - 292631..293098 (-) 468 WP_258352675.1 Lrp/AsnC ligand binding domain-containing protein -
  NWF24_RS01400 (NWF24_01400) - 293249..293707 (-) 459 WP_258352676.1 hypothetical protein -
  NWF24_RS01405 (NWF24_01405) - 293948..295222 (+) 1275 WP_258352677.1 Glu/Leu/Phe/Val dehydrogenase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41869.95 Da        Isoelectric Point: 6.4189

>NTDB_id=719817 NWF24_RS01380 WP_097197835.1 289675..290811(-) (pilU) [Variovorax paradoxus strain JBCE486]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAPHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQILKDVSMTKRGLTIMVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGQGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMRKSRNLG
MQTFDQALFDLFESHSITFEDAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=719817 NWF24_RS01380 WP_097197835.1 289675..290811(-) (pilU) [Variovorax paradoxus strain JBCE486]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGAAACGGCAGCGACTTGTTCAT
CACCGCCGACTTTCCGCCGGCCATCAAGGTCGACGGCAAGGTCACCAAAGTGTCGCAGCAGGCGCTGGGCGCGCCGCACA
CGCTGGCGCTCACGCGCTCGATCATGAACGACCGCCAGACGGCGGAGTTCGAGCGCACCAAGGAATGCAACTTCGCGATC
TCGCCGACCGGCATCGGCCGCTTCCGCGTGAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCCAAGCTGCCGACCATCGACGGCCTGGGCATGCCGCAGATTCTGAAAGACGTGTCGATGACCAAGCGCGGCCTCACCA
TCATGGTGGGCGCCACGGGTTCGGGCAAGTCGACCACGCTGGCCGCGATGATCGACTGGCGCAACGAAAATTCGTACGGC
CACATCGTGACGGTGGAAGACCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCCGTGGCCTTCGCCGAAACCGGCCACCTTTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGTCGCGCGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGGTCTCGCAGCGGCTGGTGCCAACCGAAGACGGCCAGGGCCGGGTGGCCGCTGTTGAAGTGCTGCTGAACA
CGCCGCTGATTTCCGACCTGATCTTCAAGGGCGAAGTGGGCGAGATCAAGGAGATCATGCGCAAGAGCCGCAACCTGGGC
ATGCAGACTTTCGACCAGGCGCTGTTCGACCTGTTCGAAAGCCACTCGATCACCTTCGAAGATGCCATCCGCAACGCGGA
CTCGGCAAACGACCTGCGGCTGCAGATCAAGCTGAACAGCCAGCGTGCGCGCAGCACAGACCTGGCCGCCGGCACCGAGC
ACTTCGCTATCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A286ANQ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

58.101

94.709

0.55
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

44.807

89.153

0.399
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Legionella pneumophila strain Lp02

44.073

87.037

0.384
  pilT Legionella pneumophila strain ERS1305867

44.073

87.037

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter baumannii strain A118

45.141

84.392

0.381
  pilT Acinetobacter baumannii D1279779

45.141

84.392

0.381
  pilT Acinetobacter baylyi ADP1

43.653

85.45

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362