Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NVV93_RS01540 Genome accession   NZ_CP102830
Coordinates   333230..334264 (+) Length   344 a.a.
NCBI ID   WP_258252708.1    Uniprot ID   -
Organism   Pseudomonas sp. LS44     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 328230..339264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NVV93_RS01515 (NVV93_01515) - 328568..330547 (-) 1980 WP_258252703.1 dynamin-like GTPase family protein -
  NVV93_RS01520 (NVV93_01520) - 330706..330999 (-) 294 WP_258252704.1 DUF167 domain-containing protein -
  NVV93_RS01525 (NVV93_01525) - 331022..331615 (-) 594 WP_258252705.1 YggT family protein -
  NVV93_RS01530 (NVV93_01530) proC 331626..332447 (-) 822 WP_258252706.1 pyrroline-5-carboxylate reductase -
  NVV93_RS01535 (NVV93_01535) - 332459..333166 (-) 708 WP_258252707.1 YggS family pyridoxal phosphate-dependent enzyme -
  NVV93_RS01540 (NVV93_01540) pilT 333230..334264 (+) 1035 WP_258252708.1 type IV pilus twitching motility protein PilT Machinery gene
  NVV93_RS01545 (NVV93_01545) pilU 334327..335472 (+) 1146 WP_258252709.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NVV93_RS01550 (NVV93_01550) - 335519..336118 (-) 600 WP_258254260.1 C40 family peptidase -
  NVV93_RS01555 (NVV93_01555) - 336404..336805 (+) 402 WP_258252710.1 TM2 domain-containing protein -
  NVV93_RS01560 (NVV93_01560) - 337026..338402 (+) 1377 WP_258252711.1 cystathionine beta-synthase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37938.67 Da        Isoelectric Point: 6.8890

>NTDB_id=719132 NVV93_RS01540 WP_258252708.1 333230..334264(+) (pilT) [Pseudomonas sp. LS44]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGEVFKKITDVPRGLVLVTGPTGSGKSTTLAAMLDHLNSTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQSVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KGLVAKGLVSRENAREKSKNPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=719132 NVV93_RS01540 WP_258252708.1 333230..334264(+) (pilT) [Pseudomonas sp. LS44]
ATGGATATCACCGAGCTGCTGGCCTTCAGTGCCAAGCAAGGCGCTTCAGACCTGCACCTCTCGGCAGGCTTGCCGCCGAT
GATTCGCGTCGATGGCGATGTGCGGCGGATCAACCTGCCGGCGATGGATCACAAGCAAGTACACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAATTCCTCGAGACCGACTTCTCCTTCGAAGTGCCTGGCGTGGCGCGT
TTCCGGGTCAACGCGTTCAATCAGAATCGCGGCGCCGGCGCAGTATTCCGGACCATTCCGTCCAAAGTGCTAAGCATGGA
AGACCTCGGCATGGGTGAGGTGTTCAAGAAGATCACCGACGTTCCGCGCGGTCTGGTGCTGGTCACCGGGCCGACCGGCT
CGGGTAAATCGACCACCCTGGCGGCGATGCTCGACCACCTCAACAGCACTAAATACCACCACATTCTGACCATCGAAGAC
CCGATCGAATTCGTTCACGAGTCGAAGAAGTGTCTGGTTAACCAGCGCGAAGTGCACCGCGATACCCTGGGCTTCTCCGA
GGCCTTGCGTTCGGCCCTGCGGGAAGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAGACCGGGCACTTGGTATTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCGGAAGAGAAATCCATGGTTCGCTCGATGCTCTCCGAGTCGCTGCAATCGGTGATCTCGCAGAC
TCTGCTGAAGAAGGTCGGCGGTGGCCGGGTGGCGGCCCACGAAATCATGATCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCGGCTCGCTGGGCATGCAGACCCTCGACATGTGCCTC
AAGGGCCTGGTCGCCAAGGGCCTGGTCAGCCGCGAGAATGCCCGCGAAAAATCCAAAAACCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.349

100

0.953
  pilT Pseudomonas aeruginosa PAK

95.058

100

0.951
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

81.686

100

0.817
  pilT Legionella pneumophila strain Lp02

73.837

100

0.738
  pilT Legionella pneumophila strain ERS1305867

73.837

100

0.738
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392
  pilU Vibrio cholerae strain A1552

40.299

97.384

0.392
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392