Detailed information NTG0719133

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NVV93_RS01545
• Genome accession: NZ_CP102830
• Coordinates: 334327..335472 (+)
• Length: 381 a.a.
• NCBI ID: WP_258252709.1
• Uniprot ID: -
• Organism: Pseudomonas sp. LS44
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 329327..340472

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NVV93_RS01520 (NVV93_01520)	-	330706..330999 (-)	294	WP_258252704.1	DUF167 domain-containing protein	-
NVV93_RS01525 (NVV93_01525)	-	331022..331615 (-)	594	WP_258252705.1	YggT family protein	-
NVV93_RS01530 (NVV93_01530)	proC	331626..332447 (-)	822	WP_258252706.1	pyrroline-5-carboxylate reductase	-
NVV93_RS01535 (NVV93_01535)	-	332459..333166 (-)	708	WP_258252707.1	YggS family pyridoxal phosphate-dependent enzyme	-
NVV93_RS01540 (NVV93_01540)	pilT	333230..334264 (+)	1035	WP_258252708.1	type IV pilus twitching motility protein PilT	Machinery gene
NVV93_RS01545 (NVV93_01545)	pilU	334327..335472 (+)	1146	WP_258252709.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NVV93_RS01550 (NVV93_01550)	-	335519..336118 (-)	600	WP_258254260.1	C40 family peptidase	-
NVV93_RS01555 (NVV93_01555)	-	336404..336805 (+)	402	WP_258252710.1	TM2 domain-containing protein	-
NVV93_RS01560 (NVV93_01560)	-	337026..338402 (+)	1377	WP_258252711.1	cystathionine beta-synthase	-
NVV93_RS01565 (NVV93_01565)	-	338399..339586 (+)	1188	WP_258252712.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42391.66 Da
• Isoelectric Point: 6.4210

>NTDB_id=719133 NVV93_RS01545 WP_258252709.1 334327..335472(+) (pilU) [Pseudomonas sp. LS44]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNAMSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLDELKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIKKGEVHELKALMKRSTELGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSETDGEHLTSVSQGLSLEVSEDDPGRRFS

Nucleotide

• Length: 1146 bp

>NTDB_id=719133 NVV93_RS01545 WP_258252709.1 334327..335472(+) (pilU) [Pseudomonas sp. LS44]
ATGGAATTTGAAAAGCTGCTGCGTCTGATGGTGGAAAAGGGCGGTTCCGACCTGTTCATTACCGCCGGTGTCCCGCCGTC
GATGAAGGTCAATGGCAAGATCATGCCGGTGACCAAGAACGCCATGTCACCGGAGCAGACTCGCGAAACCGTGCTCGGCG
TGATGAACGAACAGCAGCGCCGCGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCGCGCGGGATCGGCCGT
TTCCGGGTCAGCGCGTTTTATCAGCGCAACTTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCGACGCTCGA
CGAACTCAAACTGCCGGAAATTCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGCTGTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAATACATCCACCAGCATCAGAGCTGCATCGTCACCCAGCGCGAAGTGGGTATCGACACCGAGTCCTTCGAAGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGGTGCGTACCCGCGAGACCATGGACCACG
CGGTGGCCTTCGCCGAAACTGGCCACCTGTGCCTGGCCACTTTGCACGCCAACAACGCCAACCAGGCGCTCGACCGGATC
ATCAACTTCTTCCCCGCCGATCGGCAGCAGCAGGTGTGGATGGATTTGTCGTTGAACCTCAAGGCGATCGTCGCCCAGCA
GCTGATTCCGACCCCGGACGGTAAAGGCCGTCGCGCGGTGATCGAGGTGTTGATCAACACCCCGCTGGCCGCCGATCTGA
TCAAGAAAGGCGAGGTGCACGAACTCAAAGCGTTGATGAAGCGCTCCACCGAGCTGGGCATGCAGACCTTCGACCAAGCG
CTGTACAACCTCTACAGCCAGGGCGAGATCACCTACGAAGATGCCTTGCTGTATGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGCTCGGAAACCGATGGCGAGCACCTGACCAGCGTCAGTCAGGGGCTGAGCCTGGAAGTCAGCGAAG
ATGATCCGGGACGGCGGTTTAGTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	92.368	99.738	0.921
pilU	Acinetobacter baylyi ADP1	64.426	93.701	0.604
pilU	Vibrio cholerae strain A1552	58.857	91.864	0.541
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.155	88.189	0.381