Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NU949_RS04995 Genome accession   NZ_CP102679
Coordinates   573902..574882 (+) Length   326 a.a.
NCBI ID   WP_072649429.1    Uniprot ID   -
Organism   Escherichia coli strain XH989     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 568902..579882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NU949_RS04965 (NU949_04965) yggM 569417..570424 (+) 1008 WP_000745217.1 DUF1202 family protein -
  NU949_RS04970 (NU949_04970) hemW 570579..571715 (-) 1137 WP_000239944.1 radical SAM family heme chaperone HemW -
  NU949_RS04975 (NU949_04975) rdgB 571708..572301 (-) 594 WP_042630986.1 XTP/dITP diphosphatase -
  NU949_RS04980 (NU949_04980) yggU 572309..572599 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  NU949_RS04985 (NU949_04985) yggT 572596..573162 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  NU949_RS04990 (NU949_04990) yggS 573180..573884 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  NU949_RS04995 (NU949_04995) pilT 573902..574882 (+) 981 WP_072649429.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NU949_RS05000 (NU949_05000) ruvX 575058..575474 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  NU949_RS05005 (NU949_05005) yqgE 575474..576037 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  NU949_RS05010 (NU949_05010) gshB 576146..577096 (-) 951 WP_000593273.1 glutathione synthase -
  NU949_RS05015 (NU949_05015) rsmE 577109..577840 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NU949_RS05020 (NU949_05020) endA 577920..578627 (-) 708 WP_000286500.1 deoxyribonuclease I -
  NU949_RS05025 (NU949_05025) yggI 578722..579219 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35918.05 Da        Isoelectric Point: 5.5860

>NTDB_id=718365 NU949_RS04995 WP_072649429.1 573902..574882(+) (pilT) [Escherichia coli strain XH989]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAQFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=718365 NU949_RS04995 WP_072649429.1 573902..574882(+) (pilT) [Escherichia coli strain XH989]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCAATTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Acinetobacter baylyi ADP1

37.037

99.387

0.368
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362