Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NT239_RS13250 Genome accession   NZ_CP102611
Coordinates   2846518..2847561 (+) Length   347 a.a.
NCBI ID   WP_373974586.1    Uniprot ID   -
Organism   Chitinibacter sp. SCUT-21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2841518..2852561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NT239_RS13220 (NT239_13235) dsbD 2841870..2843393 (-) 1524 WP_373974580.1 protein-disulfide reductase DsbD -
  NT239_RS13225 (NT239_13240) cutA 2843390..2843740 (-) 351 WP_373974581.1 divalent-cation tolerance protein CutA -
  NT239_RS13230 (NT239_13245) - 2843756..2844199 (+) 444 WP_373974582.1 FxsA family protein -
  NT239_RS13235 (NT239_13250) - 2844288..2844875 (-) 588 WP_373974583.1 YggT family protein -
  NT239_RS13240 (NT239_13255) proC 2844906..2845724 (-) 819 WP_373974584.1 pyrroline-5-carboxylate reductase -
  NT239_RS13245 (NT239_13260) - 2845721..2846431 (-) 711 WP_373974585.1 YggS family pyridoxal phosphate-dependent enzyme -
  NT239_RS13250 (NT239_13265) pilT 2846518..2847561 (+) 1044 WP_373974586.1 type IV pilus twitching motility protein PilT Machinery gene
  NT239_RS13255 (NT239_13270) pilU 2847582..2848718 (+) 1137 WP_373974587.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NT239_RS13260 (NT239_13275) - 2848745..2850973 (+) 2229 WP_373974588.1 FimV family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38276.02 Da        Isoelectric Point: 6.8841

>NTDB_id=718155 NT239_RS13250 WP_373974586.1 2846518..2847561(+) (pilT) [Chitinibacter sp. SCUT-21]
MEISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPAMDHKDVHDMVYDIMNDGQRKIYEDTLECDFSFEIPNLAR
FRVNAFVQNRGAGAVFRTIPSKVLTLEELNAPRVFQDIAMNPRGLVLVTGPTGSGKSTTLAAMINFINDNEYGHILTVED
PIEFVHQSKKCLVNQREVGPHTLSFSNALRSALREDPDVVLVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRAMLSESLRAVISQTLLKTKDGTGRVAAHEIMIGIPAIRNLIRENKIAQMYSAIQTGSAHGMQTLDQC
LQNLVQRNIVSVAEARNKAAIPDNFKG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=718155 NT239_RS13250 WP_373974586.1 2846518..2847561(+) (pilT) [Chitinibacter sp. SCUT-21]
ATGGAAATCTCGGAACTCTTAGCGTTTTCGGTCAAGAATAAAGCATCCGATTTGCATCTGTCGGCAGGCTTACCACCGAT
GATTCGCGTGCATGGTGATGTACGTCGTATTAATTTACCGGCAATGGATCACAAAGACGTACACGATATGGTGTACGACA
TCATGAATGATGGTCAGCGCAAAATTTATGAAGATACGCTTGAATGCGATTTCTCATTTGAAATTCCCAATTTGGCGCGT
TTCCGGGTAAATGCCTTTGTGCAAAACCGGGGTGCCGGTGCGGTATTTCGGACGATTCCTTCGAAAGTACTCACGCTGGA
AGAGCTCAATGCGCCGCGTGTTTTTCAAGATATTGCAATGAATCCACGCGGGCTGGTGCTGGTTACTGGGCCTACGGGGT
CAGGTAAATCAACCACCTTGGCCGCGATGATTAATTTCATTAATGACAATGAATACGGCCATATCTTGACGGTGGAAGAC
CCGATCGAGTTTGTGCATCAGTCGAAAAAATGTTTGGTCAACCAACGTGAAGTCGGGCCACATACTCTGTCGTTCTCGAA
TGCATTGCGCTCAGCCTTGCGGGAAGACCCCGATGTTGTGCTGGTCGGTGAGATGCGAGACTTGGAAACCATCCGTTTGG
CCTTAACTGCGGCCGAAACGGGGCACTTGGTGTTTGGTACCTTGCATACGAGTTCTGCCGCGAAAACCATCGACCGTGTG
GTCGACGTGTTTCCTGCCGCAGAGAAAGAAATGGTTCGTGCAATGTTGTCTGAGTCATTGCGGGCGGTAATCTCGCAAAC
GCTGCTCAAAACCAAAGATGGCACAGGCCGTGTGGCTGCGCACGAGATCATGATTGGGATTCCTGCGATTCGAAATCTGA
TCCGTGAAAATAAAATTGCGCAAATGTATTCGGCGATTCAAACAGGTTCAGCGCACGGCATGCAAACTTTGGATCAGTGC
TTGCAAAATCTAGTACAACGCAACATTGTTTCTGTGGCTGAAGCGCGTAATAAAGCGGCCATTCCGGATAACTTCAAAGG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.261

99.424

0.778
  pilT Acinetobacter baumannii strain A118

78.261

99.424

0.778
  pilT Acinetobacter baumannii D1279779

78.261

99.424

0.778
  pilT Pseudomonas stutzeri DSM 10701

76.522

99.424

0.761
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Acinetobacter baylyi ADP1

75.942

99.424

0.755
  pilT Neisseria meningitidis 8013

72.861

97.695

0.712
  pilT Legionella pneumophila strain Lp02

72.566

97.695

0.709
  pilT Neisseria gonorrhoeae MS11

72.566

97.695

0.709
  pilT Legionella pneumophila strain ERS1305867

72.566

97.695

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.047

97.406

0.663
  pilT Vibrio cholerae strain A1552

68.047

97.406

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403
  pilU Acinetobacter baylyi ADP1

39.193

100

0.392